The New Strain of Acetic Acid Bacteria Komagataeibacter xylinus B-12068 – Producer of Bacterial Cellulose for Biomedical Applications

Shidlovsky, Ivan P.; Prudnikova, Svetlana V.; Шидловский, И.П.

doi:10.17516/1997-1389-0017

Cited by 8 publications

(6 citation statements)

References 8 publications

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“…The pre-culture was performed on the HS agar. Then, the colonies were transferred into the flask containing liquid HS medium and cultivated for 7 days at a temperature of 30°C under static conditions as described elsewhere [33]. To remove remnants of bacterial cells and components of culture medium, BC films were treated with 1.0 M NaOH at 70 °C, followed by washing in deionized water.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics

et al. 2018

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The present study describes production of bacterial cellulose composites with silver nanoparticles and antibiotics and compares their properties. Bacterial cellulose (BC) composites synthesized in the culture of the strain of acetic acid bacterium Komagataeibacter xylinus VKPM B-12068 with silver nanoparticles, BC/AgNps, were produced hydrothermally, under different AgNO 3 concentrations (0.0001, 0.001, and 0.01 M) in the reaction medium. The presence of silver in the BC/AgNp composites was confirmed by elemental analysis conducted using scanning electron microscopy with a system of X-ray spectral analysis. Analysis showed that the average atomic number of silver particles in composite samples depended on the concentration of AgNO 3 : as AgNO 3 concentration in the reaction solution was increased, silver content in the composites increased from 0.044 to 0.37 mg/cm 2. BC composites with amikacin and ceftriaxone were prepared by immersing dry BC films in solutions containing different concentrations of the antibiotics. The surface structure and properties and physicochemical and mechanical characteristics of composites were investigated using SEM, DSC, X-ray analysis, the system for measuring water contact angles, and electromechanical tensile testing machine. The disk-diffusion method and the shake-flask culture method used in this study showed that all experimental composites had pronounced antibacterial activity against E. coli, Ps. eruginosa, K. pneumoniae, and St. aureus, and the BC/antibiotic composites were more active than BC/AgNp ones; S. aureus was the most susceptible to the effect of BC composites. No potential cytotoxicity was detected in any of the BC/AgNp composites in the NIH 3T3 mouse fibroblast cell culture, in contrast to the BC/antibiotic composites. These results suggest that BC composites constructed in the present study hold promise as dressings for managing wounds, including contaminated ones.

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Section: Methodsmentioning

confidence: 99%

Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics

et al. 2018

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“…In this area, studies aimed at the development of composite materials using bacterial cellulose (BC) as a scaffold are becoming increasingly popular [ 4 , 5 ]. Many microorganisms are able to synthesize BC, but the main producer is the bacterium Komagataeibacter xylinus [ 6 ]. From a chemical point of view, BC is a linear polymer consisting of elementary units of anhydro-d-glucopyranose connected by 1,4-β-glycosidic bonds, and, since it is an exopolysaccharide synthesized by microorganisms, it is a chemically pure extracellular product that does not contain lignin, resins, fats, and waxes [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Advanced “Green” Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy

et al. 2022

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Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used. The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms. The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity. In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan. BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium. The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy. Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties. The developed approach for “grafting” of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.

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“…As shown in figure 2, K. intermedius was found to be unable to grow in the both concentrations of NaCl even after 6 days of incubation. Komagataeibacter are known for being unable to tolerate more than 0.5% NaCl (Prudnikova & Shidlovsky, 2017). It is likely that the sodium ion affected the bacteria's plasma membrane properties and therefore the bacteria's viability (Gandhi et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Development of Selective Media for Komagataeibacter intermedius and Dekkera bruxellensis from a Mixed Culture

Pratama

Devanthi

Kho

2021

Nusantara Science and Technology Proceedings

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Komagataeibacter intermedius and Dekkera bruxellensis are some of the most commonly found bacteria and yeast, respectively, during Kombucha fermentation. The development of selective media for separating both species is essential to make an accurate cell enumeration. This study aimed to develop a selective media for the enumeration of K. intermedius and D. bruxellensis from a mixed culture sample. Hestrin and Schramm (HS) agar and Potato Dextrose Agar (PDA) were chosen as the enumeration media for K. intermedius and D. bruxellensis, respectively. To select for K. intermedius, HS agar was added with various concentrations of cycloheximide (0, 1, and 10 mg/mL), NaCl (2% and 5%), and acetic acid (1%). To select for D. bruxellensis, PDA was added with various concentrations of chloramphenicol (0, 0.3, and 3 μg/mL) and NaCl (2% and 5%). Each culture (~10 6 log CFU/mL) was serially diluted and plated on the respective agar media, followed by incubation at 30°C for 1-6 days. The results showed that PDA containing 2% NaCl could completely suppress K. intermedius growth while permitting complete recovery of D. bruxellensis. However, it took 6 days until visible growth was observed by D. bruxellensis. Moreover, HS with the addition of 1% acetic acid successfully inhibited D.bruxellensis while allowing the complete recovery of K. intermedius. The findings suggested that the two media were suitable for separating K. intermedius and D. bruxellensis on agar media, thus allowing a more accurate cell counting in studies involving the mixed cultures of both species.

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The New Strain of Acetic Acid Bacteria Komagataeibacter xylinus B-12068 – Producer of Bacterial Cellulose for Biomedical Applications

Abstract: The strain of acetic acid bacteria Komagataeibacter xylinus B-12068, producing of bacterial cellulose (BC), was isolated and described. The effects of cultivation conditions (carbon sources

Cited by 8 publications

References 8 publications

Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics

Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics

Advanced “Green” Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy

Development of Selective Media for Komagataeibacter intermedius and Dekkera bruxellensis from a Mixed Culture

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