The neuroprotective effect of ONO-1714 on NMDA-mediated cytotoxicity in vitro

Characterization of the transportation of berberine in Coptidis rhizoma extract through rat primary cultured cortical neurons

“…Primary cultures of rat cerebral neurons were prepared from cerebral cortex of 1-day-old newborn rats as described previously (Shibuta et al, 2003;Varathan et al, 2001). Neurons were plated at a density of 5 × 10 5 cells/mL on poly-L-lysineprecoated 24-well plates.…”

Small temperature variations alter edaravone-induced neuroprotection of cortical cultures exposed to prolonged hypoxic episodes

“…This suspension was placed on a poly-L-lysine-coated 35 mm diameter tissue culture dish (1.5 ml per well) with a 2 mm grid (Nunc, Naperville, IL, USA) used to observe the same neurones in a given area over time. 16 After 4 days in culture, cells were treated with 5 mg ml 21 of 5-FU for 3 days to prevent proliferation of non-neuronal cells. The neurones were maintained in DMEM containing 8% FCS and 4% HS in an atmosphere of 5% CO 2 and 95% air, and under 100% humidity at a temperature of 378C.…”

Protective effects of the free radical scavenger edaravone against glutamate neurotoxicity in nearly pure neuronal culture

“…Red, neurons; green, glial cells. The proportion of red cells (neurons) was more than 90% [20,21]. Photomicrographs of three or four areas within the dish were taken shortly before the exposure to glutamate.…”