The National BioResource Project Medaka (NBRP Medaka): An Integrated Bioresource for Biological and Biomedical Sciences

Sasado, Takao; Tanaka, Minoru; Kobayashi, Keiichiro; Satô, Tadashi; Sakaizumi, Mitsuru; Naruse, Kiyoshi

doi:10.1538/expanim.59.13

Cited by 45 publications

(35 citation statements)

References 49 publications

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“…Here, we used 76 individuals belonging to the CAB strain from Carolina Biological Supply Company (Burlington, NC, USA; from AMAGEN, Gif-sur-Yvette, France) and WatchFrog (Evry, France). Fish of this standard strain present differences in the colour of eyes and other tissues (Loosli et al, 2000;Furutani-Seiki and Wittbrodt, 2004;Sasado et al, 2010;Kinoshita et al, 2009), but can be used in ecophysiological studies because their development characteristics are similar to those of the wildtype (Sasado et al, 2010;Lawrence et al, 2012). They were studied from 4 months old (mature) to 8 months old [almost the end of life at 27°C (Shima and Mitani, 2004;Leaf et al, 2011)].…”

Section: Biological Materials and Rearing Conditionsmentioning

confidence: 99%

Non-stressful temperature effect on oxidative balance and life history traits in adults fish (Oryzias latipes)

Hemmer-Brepson¹,

Replumaz

Romestaing

et al. 2013

Journal of Experimental Biology

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Temperature is well known to affect many biological and ecological traits, especially in ectotherms. From a physiological point of view, temperature is also positively correlated to metabolism and is often associated with an increase in reactive oxygen species (ROS) production. It has recently been suggested that ROS play a role in lifespan and resource allocation. However, only a few authors have attempted to explore the relationships between temperature, resource allocation and oxidative balance in ectotherms. Here, we measured the effect of temperature on growth, reproductive effort, offspring quantity and quality, hatching and survival rates, and the associated proximal costs, which were evaluated through the quantification of oxidative balance elements. We reared adult fish (Oryzias latipes) at two non-stressful temperatures (20 and 30°C) during a relatively long period (4 months, approximately the entire adult life). The results show a trade-off between reproduction and maintenance because investment toward growth could be neglected at the adult stage (confirmed by our results). Intriguingly, ROS-dependent damages did not differ between the two groups, probably because of the higher rate of activation of the antioxidant enzyme superoxide dismutase for warm-acclimated fish. The allocation toward antioxidant defences is associated with an earlier reproduction and a lower quality of offspring. These interesting results bring new perspectives in terms of the prediction of the impact of global warming on biota through the use of ecological theories based on oxidative balance and metabolism.

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Section: Biological Materials and Rearing Conditionsmentioning

confidence: 99%

Non-stressful temperature effect on oxidative balance and life history traits in adults fish (Oryzias latipes)

Hemmer-Brepson¹,

Replumaz

Romestaing

et al. 2013

Journal of Experimental Biology

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“…V L and J L segments of medaka were identified by several criteria including the presence of canonical (allowing one or two nucleotide mismatches) RSS, by the presence of AG/GT splice sides flanking open reading frames, and pattern searches for RSS with 23 or 12 bp spacers flanking the 3′ or 5′ends of gene segments. Exon boundaries were further deduced by using alignments of O. latipes EST sequences (retrieved from NCBI and http://www.shigen.nig.ac.jp/medaka) (Sasado et al 2010) to the resultant annotated scaffolds.…”

Section: Methodsmentioning

confidence: 99%

Immunoglobulin light chains in medaka (Oryzias latipes)

et al. 2013

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The gene segments encoding antibodies have been studied in many capacities and represent some of the best-characterized gene families in traditional animal disease models (mice and humans). To date, multiple immunoglobulin light chain (IgL) isotypes have been found in vertebrates and it is unclear as to which isotypes might be more primordial in nature. Sequence data emerging from an array of fish genome projects is a valuable resource for discerning complex multigene assemblages in this critical branch point of vertebrate phylogeny. Herein, we have analyzed the genomic organization of medaka (Oryzias latipes) IgL gene segments based on recently released genome data. The medaka IgL locus located on chromosome 11 contains at least three clusters of IgL gene segments comprised of multiple gene assemblages of the kappa light chain isotype. These data suggest that medaka IgL gene segments may undergo both intra- and inter-cluster rearrangements as a means to generate additional diversity. Alignments of expressed sequence tags to concordant gene segments which revealed each of the three IgL clusters are expressed. Collectively, these data provide a genomic framework for IgL genes in medaka and indicate that Ig diversity in this species is achieved from at least three distinct chromosomal regions.

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“…Hd-rR medaka strain was obtained from National Institute for Basic Biology, Okazaki, Japan, through the National BioResource Project (NBRP Medaka), Japan (Sasado et al 2010). Husbandry of medaka fish was based on Masato et al (2009).…”

Section: Transgenic Line and Fish Husbandrymentioning

confidence: 99%

Differential GFP Expression Patterns Induced by Different Heavy Metals in Tg(hsp70:gfp) Transgenic Medaka (Oryzias latipes)

et al. 2015

Mar Biotechnol

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Heat shock protein 70 (Hsp70) is one of the most widely used biomarker for monitoring environment perturbations in biological systems. To facilitate the analysis of hsp70 expression as a biomarker, we generated a Tg(hsp70:gfp) transgenic medaka line in which green fluorescence protein (GFP) reporter gene was driven by the medaka hsp70 promoter. Here, we characterized Tg(hsp70:gfp) medaka for inducible GFP expression by seven environment-relevant heavy metals, including mercury, arsenic, lead, cadmium, copper, chromium, and zinc. We found that four of them (mercury, arsenic, lead, and cadmium) induced GFP expression in multiple and different organs. In general, the liver, kidney, gut, and skin are among the most frequent organs to show induced GFP expression. In contrast, no detectable GFP induction was observed to copper, chromium, or zinc, indicating that the transgenic line was not responsive to all heavy metals. RT-qPCR determination of hsp70 mRNA showed similar induction and non-induction by these metals, which also correlated with the levels of metal uptake in medaka exposed to these metals. Our observations suggested that these heavy metals have different mechanisms of toxicity and/ or differential bioaccumulation in various organs; different patterns of GFP expression induced by different metals may be used to determine or exclude metals in water samples tested. Furthermore, we also tested several non-metal toxicants such as bisphenol A, 2,3,7,8-tetrachlorodibenzo-pdioxin, 4-introphenol, and lindane; none of them induced significant GFP expression in Tg(hsp70:gfp) medaka, further suggesting that the inducibility of Tg(hsp70:gfp) for GFP expression is specific to a subset of heavy metals.

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The National BioResource Project Medaka (NBRP Medaka): An Integrated Bioresource for Biological and Biomedical Sciences

Cited by 45 publications

References 49 publications

Non-stressful temperature effect on oxidative balance and life history traits in adults fish (Oryzias latipes)

Non-stressful temperature effect on oxidative balance and life history traits in adults fish (Oryzias latipes)

Immunoglobulin light chains in medaka (Oryzias latipes)

Differential GFP Expression Patterns Induced by Different Heavy Metals in Tg(hsp70:gfp) Transgenic Medaka (Oryzias latipes)

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