The mutagenic effect of tobacco smoke on male fertility

Omolaoye, Temidayo S.; El-Shahawy, Omar; Skosana, Bongekile T.; Boillat, Thomas; Loney, Tom; Plessis, Stefan S. du

doi:10.1007/s11356-021-16331-x

Cited by 31 publications

(37 citation statements)

References 112 publications

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“…Besides the above-mentioned endogenous sources of ROS, a growing list of potential lifestyles and environmental factors have been suggested to contribute to the extreme generation of ROS in semen. These include cigarette smoking ( 71 ), alcohol consumption ( 72 ) and electromagnetic radiations ( 73 ).…”

Section: Origins Of Ros In Semenmentioning

confidence: 99%

Oxidative Stress and Male Infertility: Evidence From a Research Perspective

Ayad

Omolaoye

Louw

et al. 2022

Front. Reprod. Health

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Male fertility potential can be influenced by a variety of conditions that frequently coincide. Spermatozoa are particularly susceptible to oxidative damage due to their limited antioxidant capacity and cell membrane rich in polyunsaturated fatty acids (PUFAs). The role of oxidative stress (OS) in the etiology of male infertility has been the primary focus of our Stellenbosch University Reproductive Research Group (SURRG) over the last 10 years. This review aims to provide a novel insight into the impact of OS on spermatozoa and male reproductive function by reviewing the OS-related findings from a wide variety of studies conducted in our laboratory, along with those emerging from other investigators. We will provide a concise overview of the production of reactive oxygen species (ROS) and the development of OS in the male reproductive tract along with the physiological and pathological effects thereof on male reproductive functions. Recent advances in methods and techniques used for the assessment of OS will also be highlighted. We will furthermore consider the current evidence regarding the association between OS and ejaculatory abstinence period, as well as the potential mechanisms involved in the pathophysiology of various systemic diseases such as obesity, insulin resistance, hypertension, and certain mental health disorders which have been shown to cause OS induced male infertility. Finally, special emphasis will be placed on the potential for transferring and incorporating research findings emanating from different experimental studies into clinical practice.

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Section: Origins Of Ros In Semenmentioning

confidence: 99%

Oxidative Stress and Male Infertility: Evidence From a Research Perspective

Ayad

Omolaoye

Louw

et al. 2022

Front. Reprod. Health

Self Cite

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“…Cumulative studies have shown that some adverse factors, such as new coronavirus [36], Marburg virus [37], chemotherapeutic drug busulfan [38], and heavy metal ion cadmium [39], can damage the BTB, resulting in spermatogenesis disorder and even male infertility. In addition, some studies have shown that some substances, such as manganese [40], nicotine and its metabolites in tobacco smoke [41], and metal oxide nanoparticles [42], can directly pass through the BTB and cause spermatogenesis disorder. Therefore, maintaining BTB integrity is an important basis for normal spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

ALKBH5 in mouse testicular Sertoli cells regulates Cdh2 mRNA translation to maintain blood–testis barrier integrity

et al. 2022

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Background RNA N6-methyladenosine (m6A) is involved in mammalian spermatogenesis. In both germ cells and Leydig cells, ALKBH5 regulates spermatogenesis and androgen synthesis in an m6A-dependent manner. However, it is unclear whether ALKBH5 plays a role in testicular Sertoli cells, which constitute the blood–testis barrier (BTB) through cell junctions between adjacent Sertoli cells. Methods ALKBH5 expression in the testes of humans and mice was detected by immunohistochemical staining and immunofluorescence staining. BTB integrity was evaluated by BTB assay. m6A-seq was performed to screen for BTB-related molecules regulated by ALKBH5. m6A immunoprecipitation–quantitative real-time polymerase chain reaction (qPCR), RNA immunoprecipitation–qPCR, western blot, coimmunoprecipitation, and polysome fractionation–qPCR analyses were performed to explore the mechanisms of ALKBH5 in BTB. Transmission electron microscopy was applied to observe the BTB ultrastructure. Results ALKBH5 in Sertoli cells is related to the integrity of the BTB. Subsequently, the m6A level on Cdh2 mRNA, encoding a structural protein N-cadherin in the BTB, was found to be regulated by ALKBH5. IGF2BP1/2/3 complexes and YTHDF1 promoted Cdh2 mRNA translation. In addition, we found that basal endoplasmic specialization, in which N-cadherin is a main structural protein, was severely disordered in the testes of Alkbh5-knockout mice. Conclusions Our study revealed that ALKBH5 regulates BTB integrity via basal endoplasmic specialization by affecting Cdh2 mRNA translation.

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“…The inhibited formation of FSH and LH results in the process of spermatogenesis being abnormal. 25 Data obtained from the one-way ANOVA test showed a significant difference in the thickness of seminiferous tubules epithelial between the positive control group and the treatment group with a significance level of 0.000. This significant difference in the thickness of seminiferous tubules was due to the administration of Javanese ginseng root extract in the treatment group.…”

Section: Discussionmentioning

confidence: 96%

Effects of Javanese Ginseng Root Extracts (Talinum triangulare W.) on Thickness of Seminiferous Tubules of Male Rats (Rattus norvegicus) Exposed by Cigarette Smoke

Nur

Illahika

Andari

2023

JUXTA

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Highlights:1. Infertility in men can be caused by smoking, the content of free radicals in cigarettes can affect male reproductive organs.2. Javanese ginseng root (Talinum triangulare w.) extract contains ginsenoside which is a natural antioxidant to reduce free radical levels inside the body.3. Javanese ginseng root (Talinum triangulare w.) extract was able to reduce free radical levels as seen from the appearance of the seminiferous tubules of male white rats exposed to cigarette smoke. AbstractIntroduction: Cigarette smoke contains various radical chemical compounds that can increase oxidative stress, which may cause lipid peroxidation in the body and affect various different organs. This study aimed to examine the effects of Javanese ginseng root extracts (Talinum triangulare W.) on the thickness of seminiferous tubules of white male rats (Rattus norvegicus) exposed to cigarette smoke.Methods: This was an experimental study with a randomized controlled method and a post-test control group design. Post-test control group design with 15 white male rats was divided into five groups: a normal group, a control group (exposed to cigarette smoke), and three treatment groups (exposed to cigarette smoke and Javanese ginseng root extract with doses of 12.5mg, 25mg, and 50mg/200grBW, respectively) for 30 days. The seminiferous tubule thickness was observed using H&E staining under a light microscope with 200x magnification in five fields view.Results: One-way ANOVA showed that Javanese ginseng root extracts significantly affected seminiferous tubule thickness (p = 0.000). With a dose of 12.5mg/200mgBW, Javanese ginseng root extract started to show a significant effect (p = 0.000) on the thickness of the epithelium of the seminiferous tubules. The group exposed to cigarette smoke had an average thickness of seminiferous tubules of 7.92 mm, the normal group had an average thickness of 9.13 mm, and treatment groups with doses 12.5mg, 25mg, and 50mg/200gBW had an average thickness of 11.85 mm, 12.7mm, and 14.69 mm, respectively.Conclusion: Javanese ginseng root extract increased seminiferous tubule thickness in white male rats exposed to cigarette smoke.

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The mutagenic effect of tobacco smoke on male fertility

Cited by 31 publications

References 112 publications

Oxidative Stress and Male Infertility: Evidence From a Research Perspective

Oxidative Stress and Male Infertility: Evidence From a Research Perspective

ALKBH5 in mouse testicular Sertoli cells regulates Cdh2 mRNA translation to maintain blood–testis barrier integrity

Effects of Javanese Ginseng Root Extracts (Talinum triangulare W.) on Thickness of Seminiferous Tubules of Male Rats (Rattus norvegicus) Exposed by Cigarette Smoke

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