The Mouse Limb Anatomy Atlas: An interactive 3D tool for studying embryonic limb patterning

DeLaurier, April; Burton, Nick; Bennett, Michael; Baldock, Richard; Davidson, Duncan; Mohun, Timothy J.; Logan, Malcolm

doi:10.1186/1471-213x-8-83

Cited by 58 publications

(74 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scapula/pelvis (grey), humerus/femur (blue), radius and ulna/tibia and fibula (red), carpals and phalanges/tarsals and phalanges (orange); arrow indicates the patella. Images adapted from the interactive 3D mouse limb anatomy atlas http://www.nimr.mrc.ac.uk/3dlimb (Delaurier et al, 2008). c: Colour coding used to describe the serially homologous bones of the limb in b and d. e, f: Immunostaining, using an anti-muscle Myosin antibody, illustrating the forelimb (e) and hindlimb (f) -type muscle patterns in (E) 15.5 Limbs.…”

Section: A Broad Region Of the Lpm Has Limb-forming Potentialmentioning

confidence: 99%

Regulation of limb bud initiation and limb‐type morphology

Duboc¹,

Logan²

2011

Developmental Dynamics

View full text Add to dashboard Cite

While the paired forelimb and hindlimb buds of vertebrates are initially morphologically homogeneous, as the limb progenitors differentiate, each individual tissue element attains a characteristic limb-type morphology that ultimately defines the constitution of the forelimb or hindlimb. This review focuses on contemporary understanding of the regulation of limb bud initiation and formation of limb-type specific morphologies and how these regulatory mechanisms evolved in vertebrates. We also attempt to clarify the definition of the terms limb-type identity and limb-type morphology that have frequently been used interchangeably. Over the last decade, three genes, Tbx4, Tbx5, and Pitx1, have been extensively studied for their roles in limb initiation and determining limb-type morphologies. The role of Tbx4 and Tbx5 in limb initiation is clearly established. However, their putative role in the generation of limb-type morphologies remains controversial. In contrast, all evidence supports a function for Pitx1 in determination of hindlimb morphologies.

show abstract

Section: A Broad Region Of the Lpm Has Limb-forming Potentialmentioning

confidence: 99%

Regulation of limb bud initiation and limb‐type morphology

Duboc¹,

Logan²

2011

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…The emergence of new tools and reagents has lead to significant progress in recent years. For example, advancement in three dimensional imaging (such as the Optical Projection Tomography or High Resolution Episcopic Microscopy; Sharpe et al, 2002;Weninger and Mohun, 2002) has facilitated such studies and allowed investigation of dynamic tissue patterning in the space of an organ such as the limb (e.g., DeLaurier et al, 2006DeLaurier et al, , 2008Boot et al, 2008;Hasson et al, 2010). Furthermore, identification of specific markers such as Tcf4 (MCT), scx (tendons), or the newly identified fibin2 (tendons), (Schweitzer et al, 2001;Kardon et al, 2003;Pearse et al, 2009) promotes the dissection of the specific pathways that regulate muscle and tendon patterning and the relations between them.…”

Section: Perspectivesmentioning

confidence: 99%

“Soft” tissue patterning: Muscles and tendons of the limb take their form

Hasson

2011

Developmental Dynamics

View full text Add to dashboard Cite

The musculoskeletal system grants our bodies a vast range of movements. Because it is mainly composed of easily identifiable components, it serves as an ideal model to study patterning of the specific tissues that make up the organ. Surprisingly, although critical for the function of the musculoskeletal system, understanding of the embryonic processes that regulate muscle and tendon patterning is very limited. The recent identification of specific markers and the reagents stemming from them has revealed some of the molecular events regulating patterning of these soft tissues. This review will focus on some of the current work, with an emphasis on the roles of the muscle connective tissue, and discuss several key points that addressing them will advance our understanding of these patterning events.

show abstract

“…OPT was introduced 2002 by James Sharp [68] (see also http://genex.hgu.mrc.ac.uk/OPT_Microscopy/optwebsite/fro ntpage/index.htm) and became an immediate success [69][70][71][72][73][74][75]. Within a few years it was commercialised and relatively easy to handle OPT scanners can be purchased from several companies.…”

Section: Optical Projection Tomography (Opt)mentioning

confidence: 99%

“…This sets an upper specimen size limit. Mouse embryos, especially early embryos are small enough to become 3D visualised with OPT [73,[78][79][80]. Late mouse embryos and mouse fetus must be parted or dissected prior to staining and scanning (e.g.…”

Section: Optical Projection Tomography (Opt)mentioning

confidence: 99%

Three-Dimensional (3D) Visualisation of the Cardiovascular System of Mouse Embryos and Fetus

Weninger¹,

Geyer²

2009

TOCARIJ

View full text Add to dashboard Cite

Abstract:The mouse is the most appropriate biomedical model organism for researching the mechanistic function of genetic factors in normal embryogenesis and in the genesis of cardiovascular malformations. Three-dimensional (3D) visualisation of the developing organs of wild type and genetically modified mouse embryos is essential for such research. This paper aims at discussing imaging methods that permit the generation of digital volume data sets, which fit for the virtual 3D visualisation and 3D analysis of the cardiovascular system of mouse embryos and mouse fetus. To cover the spectrum of imaging methods comprehensively, we will start with a short overview about early 3D-reconstruction techniques. This will be followed by a brief discussion why in vivo imaging techniques, such as ultrasound (US) or optical coherence tomography (OCT) do not fit for constructing volume data sets that permit virtual 3D visualisation of the cardiovascular system of mouse embryos/fetus. We will then briefly introduce techniques, which permit 3D analysis of the cardiovascular system but do not fit for creating digital volume data (corrosion casts, scanning electron microscopy). Finally, we will focus on describing the advantages and disadvantages, the spectrum of application and the limitations of important modern digital volume data generation methods, such as micro-computed tomography ( CT), micro-magnetic resonance imaging ( MRI), optical projection tomography (OPT), confocal microscopy, histological section based volume data generation methods, and 3D episcopic imaging methods.

show abstract

The Mouse Limb Anatomy Atlas: An interactive 3D tool for studying embryonic limb patterning

Cited by 58 publications

References 14 publications

Regulation of limb bud initiation and limb‐type morphology

Regulation of limb bud initiation and limb‐type morphology

“Soft” tissue patterning: Muscles and tendons of the limb take their form

Three-Dimensional (3D) Visualisation of the Cardiovascular System of Mouse Embryos and Fetus

Contact Info

Product

Resources

About