The molecular mechanism of SPOROCYTELESS/NOZZLE in controlling Arabidopsis ovule development

Wei, Baoye; Zhang, Jinzhe; Pang, Changxu; Yu, Hao; Guo, Dongshu; Jiang, Hao; Ding, Mingxin; Chen, Zhuoyao; Qing, Tao; Gu, Hongya; Liu, Qu; Qin, Genji

doi:10.1038/cr.2014.145

Cited by 93 publications

(76 citation statements)

References 55 publications

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“…In addition, TCP-interacting proteins seem to participate in the modification of protein levels (Li et al, 2012;Efroni et al, 2013;Tao et al, 2013;Li and Zachgo 2013;Ho and Weigel 2014;Wei et al, 2015). However, a view of the whole network intertwined by TCP genes remains to be clarified.…”

Section: Discussionmentioning

confidence: 99%

Roles of miR319 and TCP Transcription Factors in Leaf Development

2017

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Sophisticated regulation of gene expression, including microRNAs (miRNAs) and their target genes, is required for leaf differentiation, growth, and senescence. The impact of miR319 and its target TEOSINTE BRANCHED1, CYCLOIDEA, and PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTOR (TCP) genes on leaf development has been extensively investigated, but the redundancies of these gene families often interfere with the evaluation of their function and regulation in the developmental context. Here, we present the genetic evidence of the involvement of the MIR319 and TCP gene families in Arabidopsis (Arabidopsis thaliana) leaf development. Single mutations in MIR319A and MIR319B genes moderately inhibited the formation of leaf serrations, whereas double mutations increased the extent of this inhibition and resulted in the formation of smooth leaves. Mutations in MIR319 and gain-of-function mutations in the TCP4 gene conferred resistance against miR319 and impaired the cotyledon boundary and leaf serration formation. These mutations functionally associated with CUP-SHAPED COTYLEDON genes, which regulate the cotyledon boundary and leaf serration formation. In contrast, loss-of-function mutations in miR319-targeted and nontargeted TCP genes cooperatively induced the formation of serrated leaves in addition to changes in the levels of their downstream gene transcript. Taken together, these findings demonstrate that the MIR319 and TCP gene families underlie robust and multilayer control of leaf development. This study also provides a framework toward future researches on redundant miRNAs and transcription factors in Arabidopsis and crop plants.

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Section: Discussionmentioning

confidence: 99%

Roles of miR319 and TCP Transcription Factors in Leaf Development

2017

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“…Bars = 25 µm. (E) Coexpression of 35-EXB1-RFP and 35S-GFP-SPL in tobacco leaves indicated that EXB1 were colocalized with SPL, a known nuclear transcriptional repressor (Yang et al, 1999;Wei et al, 2015). Bar = 100 µm.…”

Section: Exb1-regulated Genes Controlling Shoot Branchingmentioning

confidence: 99%

“…In the 35S-EXB1-GFP transgenic plants, clear green fluorescence was observed in the nuclei of trichome cells, demonstrating that EXB1 may be a nuclear protein ( Figures 3A to 3D). To further confirm that EXB1 is located in the nucleus, we generated 35-EXB1-RFP and 35S-SPL-GFP in which the known nuclear protein SPOROCYTELESS (SPL) was fused to GFP (Yang et al, 1999;Wei et al, 2015). Coexpression of the two fusing proteins in tobacco (Nicotiana benthamiana) leaves showed that they were colocalized in the nuclei ( Figure 3E).…”

Section: Exb1 Encodes a Wrky Transcription Factor With Transactivatiomentioning

confidence: 99%

The WRKY Transcription Factor WRKY71/EXB1 Controls Shoot Branching by Transcriptionally Regulating RAX Genes in Arabidopsis

et al. 2015

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Plant shoot branching is pivotal for developmental plasticity and crop yield. The formation of branch meristems is regulated by several key transcription factors including REGULATOR OF AXILLARY MERISTEMS1 (RAX1), RAX2, and RAX3. However, the regulatory network of shoot branching is still largely unknown. Here, we report the identification of EXCESSIVE BRANCHES1 (EXB1), which affects axillary meristem (AM) initiation and bud activity. Overexpression of EXB1 in the gain-offunction mutant exb1-D leads to severe bushy and dwarf phenotypes, which result from excessive AM initiation and elevated bud activities. EXB1 encodes the WRKY transcription factor WRKY71, which has demonstrated transactivation activities. Disruption of WRKY71/EXB1 by chimeric repressor silencing technology leads to fewer branches, indicating that EXB1 plays important roles in the control of shoot branching. We demonstrate that EXB1 controls AM initiation by positively regulating the transcription of RAX1, RAX2, and RAX3. Disruption of the RAX genes partially rescues the branching phenotype caused by EXB1 overexpression. We further show that EXB1 also regulates auxin homeostasis in control of shoot branching. Our data demonstrate that EXB1 plays pivotal roles in shoot branching by regulating both transcription of RAX genes and auxin pathways.

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“…Transcriptional repressors containing ethylene-responsive element binding factor (ERF)-associated amphiphilic repression (EAR) motifs play essential roles in control of phytohormone signaling, stress responses, and other biological processes (Ohta et al, 2001;Hiratsu et al, 2002;Szemenyei et al, 2008;Pauwels et al, 2010;Causier et al, 2012;Wei et al, 2015). A central mechanism in signal transduction of several phytohormones is the E3 ligase-mediated degradation of transcriptional repressors.…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis RING-Type E3 Ligase TEAR1 Controls Leaf Development by Targeting the TIE1 Transcriptional Repressor for Degradation

et al. 2017

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The developmental plasticity of leaf size and shape is important for leaf function and plant survival. However, the mechanisms by which plants form diverse leaves in response to environmental conditions are not well understood. Here, we identified TIE1-ASSOCIATED RING-TYPE E3 LIGASE1 (TEAR1) and found that it regulates leaf development by promoting the degradation of TCP INTERACTOR-CONTAINING EAR MOTIF PROTEIN1 (TIE1), an important repressor of CINCINNATA (CIN)-like TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors, which are key for leaf development. TEAR1 contains a typical C3H2C3-type RING domain and has E3 ligase activity. We show that TEAR1 interacts with the TCP repressor TIE1, which is ubiquitinated in vivo and degraded by the 26S proteasome system. We demonstrate that TEAR1 is colocalized with TIE1 in nuclei and negatively regulates TIE1 protein levels. Overexpression of TEAR1 rescued leaf defects caused by TIE1 overexpression, whereas disruption of TEAR1 resulted in leaf phenotypes resembling those caused by TIE1 overexpression or TCP dysfunction. Deficiency in TEAR partially rescued the leaf defects of TCP4 overexpression line and enhanced the wavy leaf phenotypes of jaw-5D. We propose that TEAR1 positively regulates CIN-like TCP activity to promote leaf development by mediating the degradation of the TCP repressor TIE1.

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The molecular mechanism of SPOROCYTELESS/NOZZLE in controlling Arabidopsis ovule development

Cited by 93 publications

References 55 publications

Roles of miR319 and TCP Transcription Factors in Leaf Development

Roles of miR319 and TCP Transcription Factors in Leaf Development

The WRKY Transcription Factor WRKY71/EXB1 Controls Shoot Branching by Transcriptionally Regulating RAX Genes in Arabidopsis

The Arabidopsis RING-Type E3 Ligase TEAR1 Controls Leaf Development by Targeting the TIE1 Transcriptional Repressor for Degradation

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