The molecular basis of salt adaptation in Methanosarcina mazei Gö1

Spanheimer, Regina; Müller, Volker

doi:10.1007/s00203-008-0363-9

Cited by 29 publications

(12 citation statements)

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“…This review chronicles studies on the metabolism of CO by methanogens from 1931 to the present. For a more comprehensive general understanding of methanogens, the reader is referred to several recent review articles describing the ecology, physiology, and biochemistry of methanogenesis (Ferry 2008;Ferry and Lessner 2008;Liu and Whitman 2008;Oelgeschlager and Rother 2008;Spanheimer and Muller 2008;Thauer et al 2008).…”

Section: Introductionmentioning

confidence: 99%

CO in methanogenesis

Ferry

2010

Ann Microbiol

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Although CO is present in methanogenic environments, an understanding of CO metabolism by methanogens has lagged behind other methanogenic substrates and investigations of CO metabolism in non-methanogenic species. This review features studies on the metabolism of CO by methanogens from 1931 to the present. The pathways for CO metabolism of freshwater versus marine species are contrasted and the ecological implications discussed. The biochemistry and role of CO dehydrogenase/acetyl-CoA synthase in the pathway for conversion of acetate to methane and biosynthesis of cell carbon is presented. Finally, a proposal for the role of CO and primitive forms of the CO dehydrogenase/acetyl-CoA synthase in the origin and early evolution of life is discussed.

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Section: Introductionmentioning

confidence: 99%

CO in methanogenesis

Ferry

2010

Ann Microbiol

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“…Genome-wide transcriptional analyses of salt and/or osmotic stress responses have been performed for many different bacteria and archaea, including Escherichia coli (60), Bacillus subtilis (52), Shewanella oneidensis MR-1 (34), Yersinia pestis (19), Pseudomonas aeruginosa (1), Sinorhizobium meliloti (16), and Methanosarcina mazei (45,51). A broad set of differentially expressed genes was observed in these studies.…”

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confidence: 99%

Global Transcriptional, Physiological, and Metabolite Analyses of the Responses of Desulfovibrio vulgaris Hildenborough to Salt Adaptation

Zhou

Baidoo

et al. 2010

Appl Environ Microbiol

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The response of Desulfovibrio vulgaris Hildenborough to salt adaptation (long-term NaCl exposure) was examined by performing physiological, global transcriptional, and metabolite analyses. Salt adaptation was reflected by increased expression of genes involved in amino acid biosynthesis and transport, electron transfer, hydrogen oxidation, and general stress responses (e.g., heat shock proteins, phage shock proteins, and oxidative stress response proteins). The expression of genes involved in carbon metabolism, cell growth, and phage structures was decreased. Transcriptome profiles of D. vulgaris responses to salt adaptation were compared with transcriptome profiles of D. vulgaris responses to salt shock (short-term NaCl exposure). Metabolite assays showed that glutamate and alanine accumulated under salt adaptation conditions, suggesting that these amino acids may be used as osmoprotectants in D. vulgaris. Addition of amino acids (glutamate, alanine, and tryptophan) or yeast extract to the growth medium relieved salt-related growth inhibition. A conceptual model that links the observed results to currently available knowledge is proposed to increase our understanding of the mechanisms of D. vulgaris adaptation to elevated NaCl levels.Desulfovibrio vulgaris Hildenborough is a member of the anaerobic sulfate-reducing bacteria (SRB) (46) that are ubiquitous in anaerobic environments containing sulfate (43), such as gas pipelines, subsurface metal tanks, sediments, and offshore oil production facilities (6, 63). These niches can also contain high concentrations of salt (e.g., NaCl). Because of its capacity to immobilize soluble forms of toxic metals, the potential of D. vulgaris for bioremediation has been widely recognized (35, 57). In addition, Desulfovibrio species have been found to reduce metals in sediments and soils with high concentrations of NaCl and a mixture of toxic metals (3) and to cope with salt stresses that result from environmental hydration-dehydration cycles. Therefore, further understanding of mechanisms that D. vulgaris uses to survive and adapt to environments with high concentrations of salt (e.g., NaCl) may contribute to the development of successful bioremediation strategies. Such knowledge might also be useful for prediction and control of metal biocorrosion given the apparent role of this microbe in corrosion.The response of a microorganism to high salinity can be divided into two phases. The initial reaction to a sudden increase in the salt concentration is termed "salt shock," while the subsequent survival and growth in a high-salinity environment can be termed "salt adaptation." In both cases, exposure of D. vulgaris to high salinity may present two different, but related, environmental stimuli; one of these stimuli is osmotic stress, and the other is ionic stress (19). Hyperosmotic stress triggers water efflux from the cell that results in a reduction in the turgor pressure and dehydration of the cytoplasm, which increases the ion concentration in the cytosol, whereas ionic stress ca...

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“…Enzyme Q8PYE2_METMA is from Methanosarcina mazei , a methane‐producing archaeon. M. mazei is a freshwater organism that can adapt to grow at elevated salinities …”

Section: Resultsmentioning

confidence: 99%

Substrate specificity characterization for eight putative nudix hydrolases. Evaluation of criteria for substrate identification within the Nudix family

Nguyen

Park

et al. 2016

Proteins

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The nearly 50,000 known Nudix proteins have a diverse array of functions, of which the most extensively studied is the catalyzed hydrolysis of aberrant nucleotide triphosphates. The functions of 171 Nudix proteins have been characterized to some degree, although physiological relevance of the assayed activities has not always been conclusively demonstrated. We investigated substrate specificity for eight structurally characterized Nudix proteins, whose functions were unknown. These proteins were screened for hydrolase activity against a 74‐compound library of known Nudix enzyme substrates. We found substrates for four enzymes with k cat/K m values >10,000 M−1 s−1: Q92EH0_LISIN of Listeria innocua serovar 6a against ADP‐ribose, Q5LBB1_BACFN of Bacillus fragilis against 5‐Me‐CTP, and Q0TTC5_CLOP1 and Q0TS82_CLOP1 of Clostridium perfringens against 8‐oxo‐dATP and 3'‐dGTP, respectively. To ascertain whether these identified substrates were physiologically relevant, we surveyed all reported Nudix hydrolytic activities against NTPs. Twenty‐two Nudix enzymes are reported to have activity against canonical NTPs. With a single exception, we find that the reported k cat/K m values exhibited against these canonical substrates are well under 105 M−1 s−1. By contrast, several Nudix enzymes show much larger k cat/K m values (in the range of 105 to >107 M−1 s−1) against noncanonical NTPs. We therefore conclude that hydrolytic activities exhibited by these enzymes against canonical NTPs are not likely their physiological function, but rather the result of unavoidable collateral damage occasioned by the enzymes' inability to distinguish completely between similar substrate structures. Proteins 2016; 84:1810–1822. © 2016 The Authors Proteins: Structure, Function, and Bioinformatics Published by Wiley Periodicals, Inc.

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The molecular basis of salt adaptation in Methanosarcina mazei Gö1

Cited by 29 publications

References 71 publications

CO in methanogenesis

CO in methanogenesis

Global Transcriptional, Physiological, and Metabolite Analyses of the Responses of Desulfovibrio vulgaris Hildenborough to Salt Adaptation

Substrate specificity characterization for eight putative nudix hydrolases. Evaluation of criteria for substrate identification within the Nudix family

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