The mitA gene of Aspergillus fumigatus is required for mannosylation of inositol-phosphorylceramide, but is dispensable for pathogenicity

Kotz, Andrea; Wagener, Johannes; Engel, Jannis; Routier, Françoise H.; Echtenacher, Bernd; Pich, Andreas; Rohde, Manfred; Hoffmann, Petra; Heesemann, Jürgen; Ebel, Frank

doi:10.1016/j.fgb.2009.10.001

Cited by 25 publications

(31 citation statements)

References 27 publications

(50 reference statements)

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“…The addition of mannose to N-glycans may occur in the Golgi apparatus of filamentous fungi, but this maturation step seems limited and dispensable for growth (49,50). Likewise, mannosylation of IPC is not required for growth (51). In contrast, the initiation of O-glycans biosynthesis by dolichol-phosphomannose-dependent mannosyltransferases is essential for yeast and fungal growth, but, at least in yeast, their elongation in the Golgi apparatus is dispensable (52,53).…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of the Fungal Cell Wall Polysaccharide Galactomannan Requires Intraluminal GDP-mannose

Engel

Schmalhorst

Routier

2012

Journal of Biological Chemistry

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Background: Understanding the mechanisms of cell wall biogenesis is important for development of antifungal strategies. Results: Biosynthesis of the fungal polysaccharide galactomannan requires import of GDP-mannose into the Golgi apparatus. Conclusion: It differs from the biosynthesis of other fungal cell wall polysaccharides.Significance: This provides a new paradigm for cell wall polysaccharide biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Biosynthesis of the Fungal Cell Wall Polysaccharide Galactomannan Requires Intraluminal GDP-mannose

Engel

Schmalhorst

Routier

2012

Journal of Biological Chemistry

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“…Loss of expression of all Man-containing molecules in A. fumigatus by a targeted deletion of the genes involved in GDP-Man biosynthesis resulted in complete loss of virulence in macrophages and susceptible mice. [14,15] In addition, a more recent study has shown that the GDP-Man transporter (encoded by the gmtA gene) is essential in Aspergillus niger. [16] Therefore, the Aspergillus GDP-Man transporter (AspGMT), which is not found in mammalian cells, [12,13] represents a promising drug target for the development of novel anti-fungal drugs that inhibit GDP-Man translocation into the Golgi lumen required for the biosynthesis of the fungal virulence factor galactomannan.…”

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confidence: 99%

Direct Investigation of the Aspergillus GDP‐Mannose Transporter by STD NMR Spectroscopy

et al. 2011

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“…Jugular vein (Sivak et al, 2004a,b; Risovic et al, 2007), femoral vein (Wong et al, 1989), and penis vein (Overdijk et al, 1996; Reichard et al, 1997; Odds et al, 1998) were generally chosen for such a purpose in large rodent species, while lateral tail vein was privileged in mice (Odds et al, 1998). Retro-orbital vein was an alternative, but has become less frequent in recent years for ethical considerations (te Dorsthorst et al, 2005; Verweij et al, 2008; Wagener et al, 2008; Mouton et al, 2009; Dirr et al, 2010; Kotz et al, 2010; Heesemann et al, 2011). To provide free movement for the animal and to make easier the IV administration, Odds et al and Meerpoel et al connected a catheter to the ligated jugular vein via a proprietary swivel device (Odds et al, 2000; Meerpoel et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Rodent Models of Invasive Aspergillosis due to Aspergillus fumigatus: Still a Long Path toward Standardization

Désoubeaux

Cray

2017

Front. Microbiol.

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Invasive aspergillosis has been studied in laboratory by the means of plethora of distinct animal models. They were developed to address pathophysiology, therapy, diagnosis, or miscellaneous other concerns associated. However, there are great discrepancies regarding all the experimental variables of animal models, and a thorough focus on them is needed. This systematic review completed a comprehensive bibliographic analysis specifically-based on the technical features of rodent models infected with Aspergillus fumigatus. Out the 800 articles reviewed, it was shown that mice remained the preferred model (85.8% of the referenced reports), above rats (10.8%), and guinea pigs (3.8%). Three quarters of the models involved immunocompromised status, mainly by steroids (44.4%) and/or alkylating drugs (42.9%), but only 27.7% were reported to receive antibiotic prophylaxis to prevent from bacterial infection. Injection of spores (30.0%) and inhalation/deposition into respiratory airways (66.9%) were the most used routes for experimental inoculation. Overall, more than 230 distinct A. fumigatus strains were used in models. Of all the published studies, 18.4% did not mention usage of any diagnostic tool, like histopathology or mycological culture, to control correct implementation of the disease and to measure outcome. In light of these findings, a consensus discussion should be engaged to establish a minimum standardization, although this may not be consistently suitable for addressing all the specific aspects of invasive aspergillosis.

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The mitA gene of Aspergillus fumigatus is required for mannosylation of inositol-phosphorylceramide, but is dispensable for pathogenicity

Cited by 25 publications

References 27 publications

Biosynthesis of the Fungal Cell Wall Polysaccharide Galactomannan Requires Intraluminal GDP-mannose

Biosynthesis of the Fungal Cell Wall Polysaccharide Galactomannan Requires Intraluminal GDP-mannose

Direct Investigation of the Aspergillus GDP‐Mannose Transporter by STD NMR Spectroscopy

Rodent Models of Invasive Aspergillosis due to Aspergillus fumigatus: Still a Long Path toward Standardization

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