The minor house dust mite allergen Der p 13 is a fatty acid‐binding protein and an activator of a TLR2‐mediated innate immune response

Abstract

Satitsuksanoa, P. et al. (2016) The minor house dust mite allergen Der p 13 is a fatty acid binding protein and an activator of a TLR2-mediated innate immune response. Allergy, 71(10), pp. 1425-1434. (doi:10.1111/all.12899) This is the author's final accepted version.There may be differences between this version and the published version. You are advised to consult the publisher's version if you wish to cite from it.http://eprints.gla.ac.uk/118175/

The house dust mite allergen Der p 5 binds lipid ligands and stimulates airway epithelial cells through a TLR2‐dependent pathway

“…For the selection of clones transformed with Der p 5 cDNA, transformants were first selected for histidinol deshydrogenase‐auxotrophy (His+) followed by a screening for geneticin (G418) resistance through plating His+ clones on agar containing increasing concentrations of the antibiotic (250 to 2000 μg/mL). For a typical rDer p 5 expression assay, we followed the same protocols as previously described for rProDer p 1 and rDer p 13 . Methanol concentrations of 0.5% were added to the BMMY medium for the induction of rDer p 5 expression.…”

“…Ligand binding by rDer p 5 was investigated spectrofluorometrically at pH 7.3 and 4.2 as previously described using a set a fluorescent lipid probes, the most useful of which proved to be the naturally fluorescent fatty acid cis ‐parinaric acid (cPnA, Invitrogen), the fluorophore‐tagged fatty acids 11‐([5‐dimethylaminonaphthalene‐1‐sulfonylamino]) undecanoic acid (DAUDA, Invitrogen) and dansyl‐DL‐α‐aminocaprylic acid (DACA, Merck, Darmstadt, Germany), as well as the fluorescent non‐specific probe for exposed hydrophobic surfaces in proteins, 1‐anilinonapthalene‐8‐sulfonate (ANS, Merck). The bis form of ANS was also used but the results using it were essentially the same as for ANS.…”

“…We recently showed that Der p 13 and Blo t 7 are lipid binding proteins that are able to stimulate TLR2 of airway epithelial cells and that its lipid cargo appears to be influential in this activity . These data, together with those demonstrating the ability of Der p 21 to induce TLR2‐dependent activation, suggest that lipid carriage could represent a key determinant shared by several HDM allergens that contributes to initiation of allergic sensitization.…”

Activation through toll‐like receptor 2 on group 2 innate lymphoid cells can induce asthmatic characteristics

“…Among HDM allergens, Der p 13 can bind fatty acid and induce TLR2-associated immune response. 28 Considering PAM is a synthetic triacylated lipopeptide, part of HDM allergens like Der p 13 may be capable of combining with PAM and cooperate in activating TLR2. To exclude the possibility that other innate immune receptors except TLR2, TLR4 and dectin-1 are involved in these effects, further studies will be necessary.…”

Mite allergens

“…Information about a detailed allergenicity evaluation is currently available for only 10% of all IgE binding proteins in the databases. Completing this task will allow a better assessment of the pathogenic role of each mite allergen as has been recently suggested for Der p 23 [17,18] and could be great independently of its IgE-binding frequency [19][20][21]. Recognizing that regional combinations of mite allergens are more appropriate and less expensive than large panels of just IgE-binding components will also help.…”