The Microrna-143/145 Cluster in Tumors: A Matter of Where and When

Poli, Valeria; Seclì, Laura; Avalle, Lidia

doi:10.3390/cancers12030708

Cited by 21 publications

(15 citation statements)

References 117 publications

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“…MiR-143/miR-145 are well-known tumour suppressor miRNAs dysregulated in several cancer types, including CRC. 53 Consistently, low levels of miR-143-3p have been observed in stool of patients with CRC 11 12 and their upregulation in stool of VT/VN supports the lower cancer risk observed in relation to these dietary habits. 54 55 These two miRNAs target some fundamental tight junction proteins.…”

Section: Discussionmentioning

confidence: 56%

Stool microRNA profiles reflect different dietary and gut microbiome patterns in healthy individuals

Tarallo

Ferrero

Filippis

et al. 2021

Gut

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ObjectivesMicroRNA (miRNA) profiles have been evaluated in several biospecimens in relation to common diseases for which diet may have a considerable impact. We aimed at characterising how specific diets are associated with the miRNome in stool of vegans, vegetarians and omnivores and how this is reflected in the gut microbial composition, as this is still poorly explored.DesignWe performed small RNA and shotgun metagenomic sequencing in faecal samples and dietary recording from 120 healthy volunteers, equally distributed for the different diets and matched for sex and age.ResultsWe found 49 miRNAs differentially expressed among vegans, vegetarians and omnivores (adj. p <0.05) and confirmed trends of expression levels of such miRNAs in vegans and vegetarians compared with an independent cohort of 45 omnivores. Two miRNAs related to lipid metabolism, miR-636 and miR-4739, were inversely correlated to the non-omnivorous diet duration, independently of subject age. Seventeen miRNAs correlated (|rho|>0.22, adj. p <0.05) with the estimated intake of nutrients, particularly animal proteins, phosphorus and, interestingly, lipids. In omnivores, higher Prevotella and Roseburia and lower Bacteroides abundances than in vegans and vegetarians were observed. Lipid metabolism-related miR-425-3p and miR-638 expression levels were associated with increased abundances of microbial species, such as Roseburia sp. CAG 182 and Akkermansia muciniphila, specific of different diets. An integrated analysis identified 25 miRNAs, 25 taxa and 7 dietary nutrients that clearly discriminated (area under the receiver operating characteristic curve=0.89) the three diets.ConclusionStool miRNA profiles are associated with specific diets and support the role of lipids as a driver of epigenetic changes and host-microbial molecular interactions in the gut.

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Section: Discussionmentioning

confidence: 56%

Stool microRNA profiles reflect different dietary and gut microbiome patterns in healthy individuals

Tarallo

Ferrero

Filippis

et al. 2021

Gut

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“…The available data point out the capacity of HPV for triggering metastasis and inducing more aggressive phenotypes in breast carcinoma (31). Furthermore, the evidence demonstrated that miR-143/145 expression was negatively related to lymph node metastasis, and patients with lower levels of miR-143/145 expression may be subject to poor prognosis malignancies (52,53). Collectively, our findings may present a novel mechanism by which HPV provokes breast cancer metastasis.…”

Section: Discussionmentioning

confidence: 60%

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Nakhaie

Makvandi

Charostad

et al. 2021

Jundishapur J Microbiol

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Background: The DNA oncoviruses, including human papillomavirus (HPV) and Epstein-Barr virus (EBV) are among the most important infectious agents involved in breast carcinogenesis. These oncoviruses have a broad disrupting effect on cellular miRNAs and their functions, by which they contribute to carcinogenesis. Objectives: In this investigation, we evaluated the correlation between HPV and EBV and the expression level of tumor suppressor miRNAs (miR-143 and 145), clinical outcomes, and their association with stimulating inflammatory cytokines in patients with breast carcinoma. Methods: In our case-control study, 35 cancerous tissues and 35 adjacent non-cancerous tissues were collected from 35 patients. Nested-PCR was set up for the detection of HPV and EBV genomes, and RT-qPCR was used for miRNA expression in the case and control groups. In addition, serum specimens were obtained from all patients (n = 35) and healthy controls (n = 35) to determine the IL-8 serum concentration. Results: We found HPV and EBV in 14.2% (10/70) and 7% (5/70) of all samples, respectively. The distributions of positive samples in the case and control groups were 25.7% (9/35) and 2.9% (1/35) (P = 0.006) for HPV and 11.4% (4/35) and 2.9% (1/35) (P = 0.164) for EBV, respectively. Besides, RT-qPCR showed that miR-143 and miR-145 were significantly downregulated in HPV and EBV-infected cases compared to non-infected ones (P < 0.05). Data also indicated that the promotion of metastasis status was related to miR-143/145 downregulation and HPV infection (P = 0.003). No significant difference was found in serum IL-8 concentration concerning viral infections. Conclusions: Our results suggested the possible involvement of viral infections in breast carcinogenesis and adverse clinical outcomes by downregulating miR-143/145.

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“…In addition, miR-143 is a comprehensively reported tumor suppressor miRNA and has been reported to directly bind and inhibit the translation of several oncogenes (reviewed in [ 29 , 30 , 31 ]). Therefore, downregulation of miR-143 has been described in a considerable number of cancer cell lines and tumors [ 31 ], including bladder cancer [ 32 , 33 , 34 ], and miR-143 usually exhibits an expression pattern similar to that of its evolutionarily conserved microRNA cluster partner miR-145 [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA LINC02470 Sponges MicroRNA-143-3p and Enhances SMAD3-Mediated Epithelial-to-Mesenchymal Transition to Promote the Aggressive Properties of Bladder Cancer

Huang

Tsai

et al. 2022

Cancers

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Bladder cancer progression and metastasis have become major threats in clinical practice, increasing mortality and therapeutic refractoriness; recently, epigenetic dysregulation of epithelial-to-mesenchymal transition (EMT)-related signaling pathways has been explored. However, research in the fields of long noncoding RNA (lncRNA) and competing endogenous RNA (ceRNA) regulation in bladder cancer progression is just beginning. This study was designed to determine potential EMT-related ceRNA regulation in bladder cancer progression and elucidate the underlying mechanisms that provoke aggressiveness. After screening the intersection of bioinformatic pipelines, LINC02470 was identified as the most upregulated lncRNA during bladder cancer initiation and progression. Both in vitro and in vivo biological effects indicated that LINC02470 promotes bladder cancer cell viability, migration, invasion, and tumorigenicity. On a molecular level, miR-143-3p directly targets and reduces both LINC02470 and SMAD3 RNA expression. Therefore, the LINC02470–miR-143-3p–SMAD3 ceRNA axis rescues SMAD3 translation upon LINC02470 sponging miR-143-3p, and SMAD3 consequently activates the TGF-β-induced EMT process. In conclusion, this is the first study to demonstrate that LINC02470 plays a pivotally regulatory role in the promotion of TGF-β-induced EMT through the miR-143-3p/SMAD3 axis, thereby aggravating bladder cancer progression. Our study warrants further investigation of LINC02470 as an indicatively prognostic marker of bladder cancer.

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The Microrna-143/145 Cluster in Tumors: A Matter of Where and When

Cited by 21 publications

References 117 publications

Stool microRNA profiles reflect different dietary and gut microbiome patterns in healthy individuals

Stool microRNA profiles reflect different dietary and gut microbiome patterns in healthy individuals

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Long Noncoding RNA LINC02470 Sponges MicroRNA-143-3p and Enhances SMAD3-Mediated Epithelial-to-Mesenchymal Transition to Promote the Aggressive Properties of Bladder Cancer

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