The metabolism and morphology mutation response of probiotic Bacillus coagulans for lead stress

Xing, Si-Cheng; Mi, Jiandui; Chen, Jingyuan; Xiao, Lei; Wu, Yinbao; Liang, Juan Boo; Zhang, Lian-Hui; Liao, Xindi

doi:10.1016/j.scitotenv.2019.07.296

Cited by 15 publications

(10 citation statements)

References 80 publications

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“…In general, the value of FPKM is 0.1 or 1 as the threshold for determining whether genes are expressed. The software DESeq was used for normalization of the read counts from analysis of genes expression levels [47].…”

Section: Discussionmentioning

confidence: 99%

Breed Differences In The Expression Levels Of Gga-Mir-222a In Laying Hens Influenced H2S Production By Regulating Methionine Synthase Genes In Gut Bacteria

Xing

Huang

et al. 2020

Preprint

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Background: The microbiota in the cecum of laying hens was critical for host digestion metabolism and odor gas production. Recent studies have suggested that host miRNAs could regulate gene expression in the gut microbiota. The expression profiles of host-derived miRNAs in the cecal content of two laying hen breeds, Hy-line Gray and Lohmann Pink, which have dissimilar H2S production were characterized, and their possible effects on H2S production by regulating the expression of related genes in the microbiota were demonstrated. Results: The differential expression of microbial serine O-acetyltransferase, methionine synthase, aspartate aminotransferase, methionine-gamma-lyase and adenylylsulfate kinase between the two breeds resulted in lower H2S production in the Hy-line hens. The results also demonstrated miRNA exosomes in the cecal content of laying hens and the potential miRNA-target relationships between 9 differentially expressed miRNAs and 9 differentially expressed microbial genes related to H2S production were investigated, among which gga-miR-222a targeted two methionine synthase genes, Odosp_3416 and BF9343_2953. An in vitro fermentation experiment showed that gga-miR-222a upregulated the expression of these genes, which increased methionine concentrations but decreased H2S production and soluble sulfide concentrations, indicating the potential of host-derived gga-miR-222a to reduce H2S emission in laying hens. Conclusion: These findings identify both a physiologic role by which miRNA shapes the cecal microbiota of laying hens and a strategy to use host miRNAs to manipulate the microbiome and actively expressed key microbial genes to reduce H2S emission and breed environmentally friendly laying hens.

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Section: Discussionmentioning

confidence: 99%

Breed Differences In The Expression Levels Of Gga-Mir-222a In Laying Hens Influenced H2S Production By Regulating Methionine Synthase Genes In Gut Bacteria

Xing

Huang

et al. 2020

Preprint

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“…A number of research studies have used acclimated organism in obtaining adapted strains with improved biodegradable characteristic to enhance productivity [31][32][33][34][35][36]. A study conducted by Mate and Pathade [37] found that isolated microbes which earlier subjected to acclimatization with azo dye were usefully treated wastewater containing reactive dyes without giving toxicity of the biodegradation product.…”

Section: Isolation and Selection Of Ferulic Acid-producing Strainsmentioning

confidence: 99%

Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation using banana stem waste

et al. 2020

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Exploitation of soil bacteria for production of ferulic acid (FA) is extensively performed since bacteria are the largest soil community that have the potential in producing degrading enzymes. This study aims to isolate, identify and characterize the most efficient soil bacteria for high FA yield via co-culture fermentation of banana stem waste (BSW). Bacteria were isolated and screened from acclimatized mixture of soil culture and BSW. ARB programme package and biolog system were employed for identification and characterization. The results reveal that four isolates closely related to Bacillus spp. and one Lysinibacillus sp. had greater potential to produce FA in very large amounts. Specifically, the maximum FA yield of 394.76 mg/kg was achieved using co-culture of Bacillus sp. MB2, Bacillus sp. WB8A and B. pumilus strain WB1A, which was 2.5-fold higher than FA produced by single culture. The profiles of substrate utilization exposed strong hydrolyzation of pectin in those three potential cultures, while one showed strong hydrolyzation of glucuronic acid. The ability to efficiently hydrolyze the components proves that the chosen co-cultures are good sources of hydrolytic enzymes. The results suggest that the co-culture has contributed cooperative actions among the cultures to synergistically breakdown the FA linkage in BSW to produce high FA yield.

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“…Probiotics absorb lead ions and decrease their number in the gut, and several studies have reported the effectiveness of different probiotics in heavy metal ion adsorption in animals (Tian et al, 2012;Zhai et al, 2013Zhai et al, , 2017. Bacillus coagulans R11 (B. coagulans R11) is a lead-resistant bacterium isolated from lead mines in our previous studies (Xing et al, 2018(Xing et al, , 2019b. Thus, there are potential possibilities to employ B. coagulans R11 as a lead sorbent in the animal gut.…”

Section: Introductionmentioning

confidence: 99%

“…In previous studies, B. coagulans R11 was used to feed mice and laying hens that were exposed to lead. Based on the lead adsorption capacity of B. coagulans R11, lead could be removed from manure to reduce the toxicity of lead on experimental animals (Xing et al, 2019b(Xing et al, , 2020. In addition, previous studies also found that B. coagulans R11 feeding, especially with regard to lead exposure, could decrease the abundance of potential pathogens (Xing et al, 2019b(Xing et al, , 2020.…”

Section: Introductionmentioning

confidence: 99%

“…Based on the lead adsorption capacity of B. coagulans R11, lead could be removed from manure to reduce the toxicity of lead on experimental animals (Xing et al, 2019b(Xing et al, , 2020. In addition, previous studies also found that B. coagulans R11 feeding, especially with regard to lead exposure, could decrease the abundance of potential pathogens (Xing et al, 2019b(Xing et al, , 2020. Although the metabonomics of the culture medium of B. coagulans R11 showed that some compounds were the major metabolites that were also antibiotic compounds, this result was not sufficient to assess the potential pathogen inhibition ability of B. coagulans R11 in the animal gut environment due to the more-complex substrates and the influences of the gut microbiome (Xing et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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The Combination of Lead and Bacillus coagulans R11 Increased the Concentration of Alpha-Solanine in the Cecum of Laying Hens and the Pathogens Abundance Decreased

Xing¹,

Chen²,

Chen³

et al. 2020

Front. Microbiol.

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Alpha-solanine is an alkaloid that can inhibit the growth of pathogens and cancer cells, the present study proved that feeding with Bacillus coagulans R11 increases the concentration of alpha-solanine in the cecum of laying hens, which also decreases the abundance of potential pathogens. In addition, the bacteria genera, metabolism pathways and its proteins involved in the biosynthesis of alpha-solanine in the cecum were also characterized. The results showed that B. coagulans R11 feeding could increase the concentration of alpha-solanine, even with lead exposure. Mevalonic acid and MEP/DOXP pathways were both participated in the biosynthesis of alpha-solanine; at the same time, the gut metabolites (S)-2-amino-6-oxohexanoate, N2-succinyl-Lornithine and the bacteria proteins atoB, ispH were shown to be crucial role in the biosynthesis of alpha-solanine in the gut. The genera Faecalibacterium sp. An77 and Faecalibacterium sp. An58 2 were important in the biosynthesis of alpha-solanine, which provided the key proteins atoB and ispH. In addition, alpha-solanine could decrease the abundance of Prevotella sp. 109 and Prevotella marshii. In conclusion, alpha-solanine could be biosynthesized by cecal microorganisms with the stimulation of B. coagulans R11 in the intestine of laying hens, in addition, alpha-solanine was the main compound which also decreased the abundance of gut potential.

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The metabolism and morphology mutation response of probiotic Bacillus coagulans for lead stress

Cited by 15 publications

References 80 publications

Breed Differences In The Expression Levels Of Gga-Mir-222a In Laying Hens Influenced H2S Production By Regulating Methionine Synthase Genes In Gut Bacteria

Breed Differences In The Expression Levels Of Gga-Mir-222a In Laying Hens Influenced H2S Production By Regulating Methionine Synthase Genes In Gut Bacteria

Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation using banana stem waste

The Combination of Lead and Bacillus coagulans R11 Increased the Concentration of Alpha-Solanine in the Cecum of Laying Hens and the Pathogens Abundance Decreased

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