The metabolic programming of stem cells

Shyh‐Chang, Ng; Ng, Huck‐Hui

doi:10.1101/gad.293167.116

Cited by 259 publications

(264 citation statements)

References 102 publications

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“…51 In this study ( Figure 7D-F), the spectral changes associated with the content and composition of carbohydrate derivatives (glycogen and other sugar molecules) are detected upon Matrigel coating (significantly with 4 mg/mL), indicating progression of mESC differentiation. The energy status of the stem cells is involved in the regulation of the differentiated/undifferentiated status.…”

Section: Discussionsupporting

confidence: 51%

The effect of extracellular matrix on the differentiation of mouse embryonic stem cells

Özdil

Güler

Açıkgöz

et al. 2019

J of Cellular Biochemistry

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Embryonic stem cells (ESCs) are promising research materials to investigate cell fate determination since they have the capability to differentiate. Stem cell differentiation has been extensively studied with various microenvironment mimicking structures to modify cellular dynamics associated with the cell‐extracellular matrix (ECM) interactions and cell‐cell communications. In the current study, our aim was to determine the effect of microenvironmental proteins with different concentrations on the capacity and differentiation capability of mouse ESCs (mESCs), combining the biochemical assays, imaging techniques, Fourier transform infrared (FTIR) spectroscopy, and unsupervised multivariate analysis. Based on our data, coating the surface of mESCs with Matrigel, used as an acellular matrix substrate, resulted in morphological and biochemical changes. mESCs exhibited alterations in their phenotype after growing on the Matrigel‐coated surfaces, including their differentiation capacity, cell cycle phase pattern, membrane fluidity, and metabolic activities. In conclusion, mESCs can be stimulated physiologically, chemically, or mechanically to convert them a new phenotype. Thus, identification of ESCs’ behavior in the acellular microenvironment could be vital to elucidate the mechanism of diseases. It might also be promising to control the cell fate in the field of tissue engineering.

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Section: Discussionsupporting

confidence: 51%

The effect of extracellular matrix on the differentiation of mouse embryonic stem cells

Özdil

Güler

Açıkgöz

et al. 2019

J of Cellular Biochemistry

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“…Metabolism has been recently recognized as an important driver of pluripotency versus differentiation of embryonic stem cells. Pluripotent stem cells in the mammalian preimplantation epiblast of embryos are in a naïve state . These stem cells become primed during development after implantation .…”

Section: Metabolism In Embryonic Stem Cellsmentioning

confidence: 99%

“…Naïve rodent preimplantation epiblasts can be cultured using media containing LIF, GSK3β, and MEK inhibitors . Mouse epiblast stem cells and human embryonic stem cells are highly glycolytic, while mouse embryonic stem cells switch from glycolysis to mitochondrial respiration as needed . Primed mouse pluripotent stem cells express high levels of glucose transporters .…”

Section: Metabolism In Embryonic Stem Cellsmentioning

confidence: 99%

“…Primed mouse pluripotent stem cells express high levels of glucose transporters . These findings in culture may reflect the situation in vivo ; prior to implantation, mouse embryos preferentially use mitochondrial oxidative phosphorylation and they shift to anaerobic glycolysis once implanted into the uterine wall . It is also possible that the conditions used to culture naïve cells contribute to their use of oxidative phosphorylation.…”

Section: Metabolism In Embryonic Stem Cellsmentioning

confidence: 99%

“…LIF can induce Stat3, which promotes transcription of mitochondrial genes . The high rates of glycolysis in the primed pluripotent stem cells may help to support their rapid expansion . Again, for these studies, the precise conditions for culturing the different types of stem cells, including levels of glucose, glutamine, could affect the measurements of glucose uptake and glycolysis rates .…”

Section: Metabolism In Embryonic Stem Cellsmentioning

confidence: 99%

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The paradox of metabolism in quiescent stem cells

Coller

2019

FEBS Letters

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The shift between a proliferating and a nonproliferating state is associated with significant changes in metabolic needs. Proliferating cells tend to have higher metabolic rates, and their metabolic profiles facilitate biosynthesis, as compared to those of nondividing cells of the same sort. Recent studies have elucidated specific molecules that control metabolic changes while cells shift between proliferation and quiescence. Embryonic stem cells, which are rapidly proliferating, tend to have metabolic patterns that are similar to those of nonstem cells in a proliferative state. Moreover, although adult stem cells tend to be quiescent, their metabolic profiles have been reported in multiple organs to more closely resemble those of proliferating than those of nondividing cells in some respects. The findings raise questions about whether there are metabolic profiles that are required for stemness, and whether these profiles relate to the metabolic properties that may be required for quiescence. Here, we review the literature on how metabolism changes upon commitment to proliferation and compare the proliferating and nonproliferating metabolic states of differentiated cells and embryonic and adult stem cells.

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Self‐Oxygenation of Tissues Orchestrates Full‐Thickness Vascularization of Living Implants

Farzin

Hassan

Teixeira

et al. 2021

Adv Funct Materials

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Bioengineering of tissues and organs has the potential to generate functional replacement organs. However, achieving the full-thickness vascularization that is required for long-term survival of living implants has remained a grand challenge, especially for clinically sized implants. During the pre-vascular phase, implanted engineered tissues are forced to metabolically rely on the diffusion of nutrients from adjacent host-tissue, which for larger living implants results in anoxia, cell death, and ultimately implant failure. Here it is reported that this challenge can be addressed by engineering self-oxygenating tissues, which is achieved via the incorporation of hydrophobic oxygen-generating micromaterials into engineered tissues. Self-oxygenation of tissues transforms anoxic stresses into hypoxic stimulation in a homogenous and tissue sizeindependent manner. The in situ elevation of oxygen tension enables the sustained production of high quantities of angiogenic factors by implanted cells, which are offered a metabolically protected pro-angiogenic microenvironment. Numerical simulations predict that self-oxygenation of living tissues will effectively orchestrate rapid full-thickness vascularization of implanted tissues, which is empirically confirmed via in vivo experimentation. Self-oxygenation of tissues thus represents a novel, effective, and widely applicable strategy to enable the vascularization living implants, which is expected to advance organ transplantation and regenerative medicine applications.

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The metabolic programming of stem cells

Cited by 259 publications

References 102 publications

The effect of extracellular matrix on the differentiation of mouse embryonic stem cells

The effect of extracellular matrix on the differentiation of mouse embryonic stem cells

The paradox of metabolism in quiescent stem cells

Self‐Oxygenation of Tissues Orchestrates Full‐Thickness Vascularization of Living Implants

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