The metabolic profile of acteoside produced by human or rat intestinal bacteria or intestinal enzyme in vitro employed UPLC-Q-TOF–MS

Cui, Qingling; Pan, Yingni; Xu, Xiaotong; Zhang, Wenjie; Xiao, Wei; Qu, Shouhe; Liu, Xiaoqiu

doi:10.1016/j.fitote.2015.12.011

Cited by 46 publications

(38 citation statements)

References 24 publications

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“…Previous studies on the metabolism of ECH and ACT in vivo converged on urine and feces (Qi et al, 2013;Wang et al, 2009), which was in accordance with reports on the metabolism of ECH and ACT incubated with intestinal bacteria (Cui et al, 2016;Li et al, 2015). However, the constituents being absorbed into blood may be responsible for certain bioactivities for most drugs.…”

Section: Introductionsupporting

confidence: 86%

Systematic characterization of the metabolites of echinacoside and acteoside from Cistanche tubulosa in rat plasma, bile, urine and feces based on UPLC‐ESI‐Q‐TOF‐MS

Cui

Pan

Bai

et al. 2016

Biomedical Chromatography

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Echinacoside (ECH) and acteoside (ACT), as the most and major active components of Cistanche tubulosa, were reported to possess cardioactive, neuroprotective and hepatocyte protective effects, as well as antibacterial, antioxidative effects. Recently, more studies have focused on their pharmacological activities. However, their metabolic profiles in vivo have not been sufficiently investigated. This study proposes an approach for rapidly identifying the complicated and unpredictable metabolites of ECH and ACT in rat plasma, bile, urine and feces, and systematically and comprehensively revealing their major metabolic pathways, based on powerful ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Plasma, bile, urine and feces were collected from rats after a single 200 mg/kg oral dose. A total of 49 metabolites were detected in rat biological samples. Through analyzing metabolites in bile samples, it was found that ECH and ACT were subjected to a marked hepatic first-pass effect in liver. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Introductionsupporting

confidence: 86%

Systematic characterization of the metabolites of echinacoside and acteoside from Cistanche tubulosa in rat plasma, bile, urine and feces based on UPLC‐ESI‐Q‐TOF‐MS

Cui

Pan

Bai

et al. 2016

Biomedical Chromatography

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“…The metabolites analysis was intended to perform in both positive and negative ion modes to get more complete information about its chemical constitutions, but the responses obtained in negative mode was very low for these analytes [30]. Apart from liver enzyme system, the intestinal enzymes and gastrointestinal bacteria also play significant roles in metabolisms and pharmacological actions of active ingredients of traditional Chinese medicines [31]. To further investigate the metabolites of WZ in vivo, metabolic analysis should be carried out in intestinal microsomes and gut microbiota system to clarify the role of intestinal enzymes and gastrointestinal bacteria in the metabolism of these active lignans.…”

Section: Discussionmentioning

confidence: 99%

Metabolic mapping of Schisandra sphenanthera extract and its active lignans using a metabolomic approach based on ultra high performance liquid chromatography with high‐resolution mass spectrometry

Zhang

Jiang

et al. 2016

J of Separation Science

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Schisandra sphenanthera, the dried ripe fruit of Schisandra sphenanthera Rehd. et Wils, is widely used as a restorative, tonic and nutrition in many countries. Wuzhi tablet, an ethanol extract preparation of Schisandra sphenanthera, is a well-known herbal medicine widely used in China. Our previous studies show that Wuzhi tablet and its active lignans significantly protect liver injury. However, its metabolic profile remains unknown in vivo and in vitro. In this study, ultra high performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry based metabolomics was employed to decipher the metabolic map of Wuzhi tablet and its active lignans. Serum (2 h) and urine (24 h) samples after a 700 mg/kg single oral dose of Wuzhi tablet, and mice liver microsome samples after incubation with its active lignans were collected and analyzed. The data were further analyzed using metabolomics and metabolite identification software. In total, 33 metabolites in vivo and 34 metabolites in vitro were identified, and six among them were new metabolites. The major metabolic reactions encompassed demethylation, hydroxylation, dehydrogenation, and epoxidation. Taken together, in vitro and in vivo studies revealed the metabolic profile of Wuzhi tablet and its active lignans and demethylation and hydroxylation were their major metabolic pathways.

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“…3-HPP (56.13%), HT (24.77%) and reduction 121 or its isomers (18.07%) were the main products of 121 produced by the action of human bacteria, while 3-HPP (55.75%) and 134 (36.31%) were the main products of 121 produced by rat bacteria. The content of metabolite produced by intestinal enzyme was lower than that produced by intestinal bacteria, which indicated that intestinal bacteria had more impact on the absorption and metabolism of 121 than that of intestinal enzyme [128,129].…”

Section: Pharmacokinetics Of Echinacoside (117) and Acteoside (121)mentioning

confidence: 95%

“…In another study, Harput et al reported calceorioside A (127) as well as 121 showed strong radical scavenging effects against DPPH, NO and superoxide anion radical comparable to that of known antioxidants [74]. Recently, DPPH¨scavenging, anti-LP assays, ABTS +¨s cavenging, OH scavenging, superoxide anion radical scavenging, Cu 2+ -chelating and FRAP assays were used to evaluate the antioxidant activities of poliumoside (128), alyssonoside (129), brandioside (130), 121, 125 and their derivatives, and the tested compounds were all screened out as antioxidants [75]. The structure-activity relationship between PhGs and their antioxidant activities indicated that the ortho-dihydroxyphenyl group was the important group, and the steric hindrance, the number as well as the position of phenolic hydroxyl were also thought to play an important effect [76].…”

Section: Antioxidant Activitymentioning

confidence: 99%

Phenylethanoid Glycosides: Research Advances in Their Phytochemistry, Pharmacological Activity and Pharmacokinetics

2016

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Phenylethanoid glycosides (PhGs) are widely distributed in traditional Chinese medicines as well as in other medicinal plants, and they were characterized by a phenethyl alcohol (C 6 -C 2 ) moiety attached to a β-glucopyranose/β-allopyranose via a glycosidic bond. The outstanding activity of PhGs in diverse diseases proves their importance in medicinal chemistry research. This review summarizes new findings on PhGs over the past 10 years, concerning the new structures, their bioactivities, including neuroprotective, anti-inflammatory, antioxidant, antibacterial and antivirus, cytotoxic, immunomodulatory, and enzyme inhibitory effects, and pharmacokinetic properties.

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The metabolic profile of acteoside produced by human or rat intestinal bacteria or intestinal enzyme in vitro employed UPLC-Q-TOF–MS

Cited by 46 publications

References 24 publications

Systematic characterization of the metabolites of echinacoside and acteoside from Cistanche tubulosa in rat plasma, bile, urine and feces based on UPLC‐ESI‐Q‐TOF‐MS

Systematic characterization of the metabolites of echinacoside and acteoside from Cistanche tubulosa in rat plasma, bile, urine and feces based on UPLC‐ESI‐Q‐TOF‐MS

Metabolic mapping of Schisandra sphenanthera extract and its active lignans using a metabolomic approach based on ultra high performance liquid chromatography with high‐resolution mass spectrometry

Phenylethanoid Glycosides: Research Advances in Their Phytochemistry, Pharmacological Activity and Pharmacokinetics

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