The mechanisms of reovirus uncoating and gene activation in vivo

Silverstein, Samuel C.; Astell, Caroline R.; Levin, Daniel H.; Schonberg, Michael; Acs, George

doi:10.1016/0042-6822(72)90571-5

Cited by 163 publications

(150 citation statements)

References 14 publications

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“…Densities of bands corresponding to 3 were divided by those corresponding to 2 as a control for loading. Core protein 2 is not degraded during protease treatment of virions to generate ISVPs (11,14,16,(43)(44)(45). To statistically compare 3/2 ratios between two different conditions, one-tailed, two-sample t tests assuming unequal variance were calculated using Excel 97 (Microsoft, Redmond, WA).…”

Section: Sds-page Of Reovirus Structuralmentioning

confidence: 99%

Cathepsin L and Cathepsin B Mediate Reovirus Disassembly in Murine Fibroblast Cells

Ebert

Deussing

Peters

et al. 2002

Journal of Biological Chemistry

263

315

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After attachment to receptors, reovirus virions are internalized by endocytosis and exposed to aciddependent proteases that catalyze viral disassembly. Previous studies using the cysteine protease inhibitor E64 and a mutant cell line that does not support reovirus disassembly suggest a requirement for specific endocytic proteases in reovirus entry. This study identifies the endocytic proteases that mediate reovirus disassembly in murine fibroblast cells. Infection of both L929 cells treated with the cathepsin L inhibitor Z-Phe-Tyr(tBu)-diazomethyl ketone and cathepsin L-deficient mouse embryo fibroblasts resulted in inefficient proteolytic disassembly of viral outer-capsid proteins and decreased viral yields. In contrast, both L929 cells treated with the cathepsin B inhibitor CA-074Me and cathepsin B-deficient mouse embryo fibroblasts support reovirus disassembly and growth. However, removal of both cathepsin B and cathepsin L activity completely abrogates disassembly and growth of reovirus. Concordantly, cathepsin L mediates reovirus disassembly more efficiently than cathepsin B in vitro. These results demonstrate that either cathepsin L or cathepsin B is required for reovirus entry into murine fibroblasts and indicate that cathepsin L is the primary mediator of reovirus disassembly. Moreover, these findings suggest that specific endocytic proteases can determine host cell susceptibility to infection by intracellular pathogens.

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Section: Sds-page Of Reovirus Structuralmentioning

confidence: 99%

Cathepsin L and Cathepsin B Mediate Reovirus Disassembly in Murine Fibroblast Cells

Ebert

Deussing

Peters

et al. 2002

Journal of Biological Chemistry

263

315

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show abstract

“…Following attachment to cell surface receptors sialic acid (9,10) and junctional adhesion molecule 1 (11), reovirus virions are internalized into cells by receptormediated endocytosis (12)(13)(14)(15). Within the endocytic pathway, host proteases catalyze the conversion of virions to infectious subvirion particles (ISVPs) by cleavage of viral outer-capsid proteins (13,(15)(16)(17). In murine fibroblasts, either of the endocytic proteases cathepsin B or cathepsin L are required for viral disassembly (3).…”

Section: Cathepsin B-green Fluorescent Protein (Gfp) Fusion Protein Ementioning

confidence: 99%

Cathepsin B Is Inhibited in Mutant Cells Selected during Persistent Reovirus Infection

Ebert

Kopecky-Bromberg

Dermody

2004

Journal of Biological Chemistry

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“…When virions are used to infect cells in culture, the a3 and ,ulC proteins of infecting virions are cleaved in similar ways as during generation of ISVPs in vitro (33, [38][39][40]. These cleavages may be initiated by proteases inside endosomes or lysosomes, which virions enter after attachment and endocytosis (33).…”

mentioning

confidence: 99%

Mechanisms of viral pathogenesis. Distinct forms of reoviruses and their roles during replication in cells and host.

Nibert¹,

Furlong²,

Fields³

1991

J. Clin. Invest.

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The mechanisms of reovirus uncoating and gene activation in vivo

Cited by 163 publications

References 14 publications

Cathepsin L and Cathepsin B Mediate Reovirus Disassembly in Murine Fibroblast Cells

Cathepsin L and Cathepsin B Mediate Reovirus Disassembly in Murine Fibroblast Cells

Cathepsin B Is Inhibited in Mutant Cells Selected during Persistent Reovirus Infection

Mechanisms of viral pathogenesis. Distinct forms of reoviruses and their roles during replication in cells and host.

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