The mechanisms and molecules involved in cytoadherence and pathogenesis of Trichomonas vaginalis

Alderete, John F.; Lehker, Michael W.; Arroyo, Rossana

doi:10.1016/0169-4758(95)80122-7

Cited by 52 publications

(61 citation statements)

References 20 publications

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“…D, depressions; P, protrusions; IP, intramembranous particles, x 48,000. two cell surfaces (1, ll, 23, 35). Much evidence suggests that cell surface molecules play key roles in mediating host-parasite interactions (1,3,16,23,32,35) and in protecting parasites in a hostile environment (27,36).…”

Section: Discussionmentioning

confidence: 99%

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Structural Changes at the Site of Tritrichomonas foetus-Erythrocyte Interaction.

Silva¹,

Filho

Souza

1996

Cell Struct. Funct.

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ABSTRACT. Tritrichomonas foetus strongly agglutinates human erythrocytes, thus suggesting the occurrence of an adhesin associated with its surface. Adherencewas observed immediately after mixing of the parasites with erythrocytes and the intensity increased for up to 30 min. Scanning electron microscopy examination of T. foetus-erythrocytes attachment showed that trichomonad cytoadherence took place mainly through their anterior and recurrent flagella. Ultrastructural observations showed that T. foetus contacts humanred blood cells through punctual binding, inducing the separation between the two lipid monolayers of the parasite plasma membrane.This structural modification was also seen in freeze-fracture replicas where protrusions on the P and depressions on the E fracture faces were observed. No intramembranous particles, which mainly correspond to membraneintegral proteins, were observed at the adhesion areas, indicating lateral mobility of integral membrane componentsinvolved in the appearance of the intramembranous particles. However,no changes was observed on the surface coat.

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Section: Discussionmentioning

confidence: 99%

“…Attachment of parasite to cells from the host is the initial event in the infection process and is considered an important virulence factor (1,3,12,14,20,21). Studies centered on the trichomonad-cell interaction have pointed out that the protozoan can exert its cytopathic effects through physical contact established between the Figs.…”

Section: Discussionmentioning

confidence: 99%

Structural Changes at the Site of Tritrichomonas foetus-Erythrocyte Interaction.

Silva¹,

Filho

Souza

1996

Cell Struct. Funct.

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“…A relationship between adhesion and cytopathogenicity was demonstrated for 77. vaginalis in cell cultures (Brasseur & Savel, 1982), and Fiori et al in 1993, showed that T. vaginalis lysed human erythrocytes by pore-forming in their membrane. Proteins involved in cytoadherence and pathogenesis of T. vaginalis have been identified (Fiori et al, 1993;Alderete et al, 1995). Although adherence of parasite on the target cell surface has been considered for long as a prerequisite to cell damage, and particularly hemolysis of erythrocytes, Fiori et al in 1996, showed that a contact independent hemolysis was mediated by a protein of more than 30 kDa released by T. vaginalis under triggering conditions.…”

Section: Discussionmentioning

confidence: 99%

Hemolytic activity ofMonocercomonasspp.

et al. 1998

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“…vaginalis infects the urogenital system of humans and recruits inflammatory cells to the infection site [3]. Besides live T. vaginalis, various statuses of trichomonads, such as weakened trophozoites fighting inflammatory cells, and ESP from trichomonads, could also influence the immune responses of inflammatory cells, including neutrophils at the site of trichomoniasis.…”

Section: Influence Of Parasite Conditions On Neutrophil Apoptosismentioning

confidence: 99%

“…The pathogenesis of trichomoniasis in humans is not yet clearly understood, although T. vaginalis is known to be a non-invasive microorganism which recruits inflammatory cells to the infection site following genital tract surface attachment [3]. Meanwhile, many neutrophils were observed in the vaginal discharge of women with trichomoniasis and a known chemotactic cytokine, IL-8, secretion was observed after T. vaginalis stimulation [4,5].…”

Section: Introductionmentioning

confidence: 99%

Delayed Human Neutrophil Apoptosis by Trichomonas vaginalis Lysate

et al. 2010

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Neutrophils play an important role in the human immune system for protection against such microorganisms as a protozoan parasite, Trichomonas vaginalis; however, the precise role of neutrophils in the pathogenesis of trichomoniasis is still unknown. Moreover, it is thought that trichomonal lysates and excretory-secretory products (ESP), as well as live T. vaginalis, could possibly interact with neutrophils in local tissues, including areas of inflammation induced by T. vaginalis in humans. The aim of this study was to investigate the influence of T. vaginalis lysate on the fate of neutrophils. We found that T. vaginalis lysate inhibits apoptosis of human neutrophils as revealed by Giemsa stain. Less altered mitochondrial membrane potential (MMP) and surface CD16 receptor expression also supported the idea that neutrophil apoptosis is delayed after T. vaginalis lysate stimulation. In contrast, ESP stimulated-neutrophils were similar in apoptotic features of untreated neutrophils. Maintained caspase-3 and myeloid cell leukemia-1 (Mcl-1) in neutrophils co-cultured with trichomonad lysate suggest that an intrinsic mitochondrial pathway of apoptosis was involved in T. vaginalis lysate-induced delayed neutrophil apoptosis; this phenomenon may contribute to local inflammation in trichomoniasis.

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The mechanisms and molecules involved in cytoadherence and pathogenesis of Trichomonas vaginalis

Cited by 52 publications

References 20 publications

Structural Changes at the Site of Tritrichomonas foetus-Erythrocyte Interaction.

Structural Changes at the Site of Tritrichomonas foetus-Erythrocyte Interaction.

Hemolytic activity ofMonocercomonasspp.

Delayed Human Neutrophil Apoptosis by Trichomonas vaginalis Lysate

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