The mechanism of indole acetic acid cytotoxicity

Melo, Mariza Pires de; Lima, Thaís Martins de; Pithon-Curi, Tânia Cristina; Curi, Rui

doi:10.1016/j.toxlet.2003.12.067

Cited by 102 publications

(78 citation statements)

References 22 publications

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“…The results presented here also showed that in the presence of IAA, both naphthoquinones at 1000 µM induced a 30% lower increase in HCF III reduction by the maize coleoptile segments compared to variant without auxin. In trying to explain this finding, we assumed that auxin, which is able to change the fluidity of the lipid systems and weaken the interactions between their components (De Melo et al 2004;Celik et al 2006) may disturb the integration of naphthoquinones into the plasma membrane (hypothesis proposed by Lüthje et al 1992) and subsequently decrease HCF III reduction. It should also be added that IAA alone stimulated HCF III reduction as was previously shown by Lekacz and Karcz (2006).…”

Section: Discussionmentioning

confidence: 99%

A comparison of the effects of 1,4-naphthoquinone and 2-hydroxy-1,4-naphthoquinone (lawsone) on indole-3-acetic acid (IAA)-induced growth of maize coleoptile cells

2017

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Section: Discussionmentioning

confidence: 99%

A comparison of the effects of 1,4-naphthoquinone and 2-hydroxy-1,4-naphthoquinone (lawsone) on indole-3-acetic acid (IAA)-induced growth of maize coleoptile cells

2017

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“…36,21 Studies have shown a down-regulation of gene expression for antioxidant enzymes from animals supplemented with antioxidant, 37 accompanied by no alteration in morphologic and biochemistry parameters. 38 On the other hand, additional studies have pointed out that a decreased enzyme activity in response to oxidative stress may accompany an initial exposure to xenobiotic, which can be followed by an induction of the antioxidant system.…”

Section: Discussionmentioning

confidence: 99%

“…21,22 Experimental protocol Thirty-two animals were divided equally into four groups with eight animals each. The control group received only PBS and the other three groups received IAA at: 50 (T 50 ), 250 T 250 ), and 500 (T 500 ) mg Kg À1 of body mass per day.…”

Section: Indole-3-acetic Acidmentioning

confidence: 99%

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Protective action of indole‐3‐acetic acid on induced hepatocarcinoma in mice

Mourão

Cruz

Paulo

et al. 2008

Cell Biochemistry & Function

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In this study, we report the protective effects of IAA on diethylnitrosamine (DEN)-induced hepatocarcinogenesis. BALB/c mice received daily IAA at 50 (T 50 ), 250 (T 250 ), and 500 (T 500 ) mg Kg À1 per body mass by gavage for 15 days. At day 15, animals were administered DEN and sacrificed 4 h later. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed in sera. In addition, hepatomorphologic alterations, activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), gene expression of antioxidant enzymes and DNA integrity were evaluated in the liver. IAA administration did not show any alterations in any of the parameters available, except for a reduction of the gene expression for antioxidant enzymes by 55, 56, 27, and 28% for SOD, CAT, GPx, and GR upon T 500 , respectively compared with the control. Several hepatic alterations were observed by DEN exposure. Moreover, IAA administration at 3 doses was shown to provide a total prevention of the active reduction of CAT and GR induced by DEN exposure compared with the control. IAA at T 500 was shown to give partial protection (87, 71, 57, and 90% for respectively SOD, CAT, GPx, and GR) on the down-regulation of the enzymes induced by DEN and this auxin showed a partial protection (50%) on DEN-induced DNA fragmentation for both parameters when compared to DEN alone. This work showed IAA hepatocarcinogenesis protection for the first time by means of a DEN-protective effect on CAT and GR activity, and by affecting antioxidant gene expression and DNA fragmentation.

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“…The effect of IAA on antioxidant enzymes is mostly concerned with animals. De Melo et al (2004) reported that rat neutrophils and lymphocytes in the presence of IAA (1 mM) increased the activities of SOD, CAT and glutathione peroxidase, which could be an attempt at self-protection.…”

Section: Introductionmentioning

confidence: 99%

Effect of indole-3-acetic acid on lead accumulation in maize (Zea mays L.) seedlings and the relevant antioxidant response

Wang

Shan

Wen

et al. 2007

Environmental and Experimental Botany

106

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The effect of indole-3-acetic acid (IAA) on lead (Pb) accumulation in maize (Zea mays L. cv. TY2) seedlings, and the responses of maize to Pb, IAA or Pb + IAA stress were investigated under hydroponic conditions. Exogenous IAA significantly increased Pb accumulation in roots, but significantly decreased Pb accumulation in shoots over the culture period of 5 days. Increased Pb uptake in maize roots partly took place through calcium and potassium channels, and also depended upon membrane potential. Lipid peroxidation in terms of malondialdehyde (MDA) content was enhanced, indicating that Pb and/or IAA caused oxidative stress in maize seedlings. Under stress conditions the antioxidant defense mechanism was activated. Some antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were up-regulated compared to the control. Pb, IAA or Pb + IAA treatments resulted in a general reduction of macro-and micronutrient contents, especially of K, Ca and Mn. Compared to a single Pb or IAA treatment, treatment with a combination of Pb + IAA induced more severe oxidative stress on maize roots and shoots as inferred from the higher observed MDA and lower nutrient contents. A Fourier transform infrared (FTIR) spectroscopic study indicated that Pb reacted with the carboxylic acid group of the IAA pyrrole cycle.

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The mechanism of indole acetic acid cytotoxicity

Cited by 102 publications

References 22 publications

A comparison of the effects of 1,4-naphthoquinone and 2-hydroxy-1,4-naphthoquinone (lawsone) on indole-3-acetic acid (IAA)-induced growth of maize coleoptile cells

A comparison of the effects of 1,4-naphthoquinone and 2-hydroxy-1,4-naphthoquinone (lawsone) on indole-3-acetic acid (IAA)-induced growth of maize coleoptile cells

Protective action of indole‐3‐acetic acid on induced hepatocarcinoma in mice

Effect of indole-3-acetic acid on lead accumulation in maize (Zea mays L.) seedlings and the relevant antioxidant response

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