THE MECHANISM OF ACTION OF MERCURIAL DIURETICS IN RATS; THE METABOLISM OF ²⁰³Hg‐LABELLED CHLORMERODRIN

Organic mercurial diuresis: Inhibition of glutamine utilization in the acidotic rat

“…Others have notcd that certain organic mer-curial~ cause a reduction in the diuretic response due to decreased glomerular filtration (Kessler et a%. 1964;Clarkson et al 1965) and this appears the case with chlsrmerodrin i~a the rat (Table 1 ) . A high sensitivity of the rat kidney to rncrcupials may in fact be the reason the rat does not respond well to the mercurials, especially in regard to PCMB (Bowman and Landoil 1967;Dzurik and Krajci-Lozary 1962).…”

“…[Traduit par Ie journal] Organic mercurials, including PCMB, are diuretic agents in the rat (Cafruny et al 1966;Clarkson et al 1965 ;R4ussini 1958) and this effect is potentiated by IVH4C1-induced metabolic acidosis (Light 1959;Lipschitz et al 1943). More recently, Pitts and asssciates (Pitts et al 1972) have shown that metabolic acidosis results in a shift in renal fuels utilized to predominantly glutamine oxidation.…”

THE MECHANISM OF ACTION OF MERCURIAL DIURETICS IN RATS; THE RENAL METABOLISM OF p‐CHLOROMERCURIBENZOATE AND ITS EFFECTS ON URINARY EXCRETION

“…TCA recovery, " column 3, is the total 203Hg in the trichloroacetic acid (TCA) extract expressed as the percentage of 203Hg in the homogenate. The last two columns are from homogenates suspended in 0.1 N-cysteine-sodium hydroxide as described by Clarkson et al (1965) (004) Chloride 0~~~~~~~~~~0 1.9 3.75 7.5 p-Chloromercuribenzoate (mg/kg mercury) Fig. 2.…”

“…Secondly the mercuric ion levels which are associated with diuresis following injection of p-chloromercuribenzoate are very close to those eliciting diuresis after a dose of chlormerodrin. Diuresis was initiated when the mercuric ion in kidney attained a threshold level of approximately 7 % of the 2.5 mg/kg mercury injected dose of chlormerodrin (Clarkson et al, 1965). This corresponds to approximately 35 pug of mercuric ion in the kidneys of 200 g rats.…”

“…99% of the p-chloromercuribenzoate and 90% of the mercuric ion were extractable from the homogenate (see column headed " TCA recovery " in Table 1). The Conway microdiffusion unit, containing metallic mercury in the central well, was used to determine the exchangeability of the 203Hg in the trichloroacetic acid extract as described by Clarkson et al (1965).…”