Biochemical Pharmacology volume 77, issue 5, P920-931 2009 DOI: 10.1016/j.bcp.2008.11.006 View full text
Shizuo Narimatsu, Daichi Kazamori, Kazufumi Masuda, Takashi Katsu, Yoshihiko Funae, Shinsaku Naito, Hironori Nakura, Shigeru Yamano, Nobumitsu Hanioka

Abstract: The capacity to oxidize bufuralol (BF) and dextromethorphan (DEX) was compared kinetically between human CYP2D6 and four rat CYP2D (CYP2D1, -2D2, -2D3 and -2D4) isoenzymes in a yeast cell expression system. In BF 1''-hydroxylation and DEX O-demethylation, only CYP2D4 showed hook-shaped Eadie-Hofstee plots, the other four CYP2D enzymes exhibiting linear plots. In DEX N-demethylation, rat CYP2D2 did not show any detectable activity under the conditions used, whereas the other four enzymes yielded linear Eadie-Ho…

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