The measurement of ultrasound scattering from individual micron-sized objects and its application in single cell scattering

Falou, Omar; Min, Rui; Kaffas, Ahmed El; Kumaradas, J. Carl; Kolios, Michael C.

doi:10.1121/1.3455795

Cited by 30 publications

(29 citation statements)

References 25 publications

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“…20 It was found that the BSC from an isolated cell was best modeled as a fluid sphere having the whole cell size. 23 Taggart et al 24 conducted high frequency ultrasound measurements on cell pellets (i.e., highly packed cells) made up of mono-and multi-nucleated cells or isolated nuclei. This study suggests that the integrated BSCs were correlated with the size of the nuclei.…”

Section: Introductionmentioning

confidence: 99%

“…Identifying the scattering sites will lead to the improvement of the theoretical scattering models. Baddour et al 21,22 and Falou et al 23 performed measurements of high frequency MHz) ultrasound BSC responses from individual cells and modeled a cell as a single spherical scatterer with uniform acoustic properties. The BSC measurements were compared to theoretical BSC predictions from a fluid sphere model 9 or from an elastic sphere model.…”

Section: Introductionmentioning

confidence: 99%

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Structure factor model for understanding the measured backscatter coefficients from concentrated cell pellet biophantoms

Franceschini

Guillermin

Tourniaire

et al. 2014

The Journal of the Acoustical Society of America

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Ultrasonic backscatter coefficient (BSC) measurements were performed on K562 cell pellet biophantoms with cell concentrations ranging from 0.006 to 0.30 in the 10-42 MHz frequency bandwidth. Three scattering models, namely, the fluid-filled sphere model (FFSM), the particle model (PM), and the structure factor model (SFM), were compared for modeling the scattering from an ensemble of concentrated cells. A parameter estimation procedure was developed in order to estimate the scatterer size and relative impedance contrast that could explain the measured BSCs from all the studied cell concentrations. This procedure was applied to the BSC data from K562 cell pellet biophantoms in the 10-42 MHz frequency bandwidth and to the BSC data from Chinese hamster ovary cell pellet biophantoms in the 26-105 MHz frequency bandwidth given in Han, Abuhabsah, Blue, Sarwate, and O'Brien [J. Acoust. Soc. Am. 130, 4139-4147 (2011)]. The data fitting quality and the scatterer size estimates show that the SFM was more suitable than the PM and the FFSM for modeling the responses from concentrated cell pellet biophantoms.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structure factor model for understanding the measured backscatter coefficients from concentrated cell pellet biophantoms

Franceschini

Guillermin

Tourniaire

et al. 2014

The Journal of the Acoustical Society of America

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“…Falou et al . reported theoretical and experimental backscattering results on elastic and fluid spheres from a micrometer-sized polystyrene object and single OCI-AML-5 cell at 25 and 55 MHz, and these results showed that backscattering from a single OCI-AML-5 cell can be modeled as an elastic and a fluid sphere [13]. The results showed a good agreement between theoretical and experimental results.…”

Section: Introductionmentioning

confidence: 83%

Ultrasonic scattering measurements of a live single cell at 86 MHz

Lee

Jung

Lam

et al. 2015

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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Cell separation and sorting techniques have been employed biomedical applications such as cancer diagnosis and cell gene expression analysis. The capability to accurately measure ultrasonic scattering properties from cells is crucial in making an ultrasonic cell sorter a reality if ultrasound scattering is to be used as the sensing mechanism as well. To assess the performance of sensing and identifying live single cells with high-frequency ultrasound, an 86-MHz lithium niobate press-focused single-element acoustic transducer was used in a high-frequency ultrasound scattering measurement system that was custom designed and developed for minimizing noise and allowing better mobility. Peak-to-peak echo amplitude, integrated backscatter (IB) coefficient, spectral parameters including spectral slope and intercept, and midband fit from spectral analysis of the backscattered echoes were measured and calculated from a live single cell of two different types on an agar surface: leukemia cells (K562 cells) and red blood cells (RBCs). The amplitudes of echo signals from K562 cells and RBCs were 48.25 ± 11.98 mVpp and 56.97 ± 7.53 mVpp, respectively. The IB coefficient was −89.39 ± 2.44 dB for K562 cells and −89.00 ± 1.19 dB for RBCs. The spectral slope and intercept were 0.30 ± 0.19 dB/MHz and −56.07 ± 17.17 dB, respectively, for K562 cells and 0.78 ± 0.092 dB/MHz and −98.18 ± 8.80 dB, respectively, for RBCs. Midband fits of K562 cells and RBCs were −31.02 ± 3.04 dB and −33.51 ± 1.55 dB, respectively. Acoustic cellular discrimination via these parameters was tested by Student’s t-test. Their values, except for the IB value, showed statistically significant difference (p < 0.001). This paper reports for the first time that ultrasonic scattering measurements can be made on a live single cell with a highly focused high-frequency ultrasound microbeam at 86 MHz. These results also suggest the feasibility of ultrasonic scattering as a sensing mechanism in the development of ultrasonic cell sorters.

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“…Currently there is no consensus on the dominant ultrasonic scattering source of biological structures. Some studies support the hypothesis that the whole cell acts as a dominant scatterer source (Falou et al 2010;Oelze and Zachary 2006). Other studies suggest that the nucleus is the main scatterer source when cells are embedded in tumors or surrounded by other cells (Czarnota et al 1999;Kolios et al 2003;Taggart et al 2007).…”

Section: Introductionmentioning

confidence: 92%