The Maize <i>lpa241</i> Mutation Causes a Remarkable Variability of Expression and Some Pleiotropic Effects

Pilu, Roberto; Landoni, M.; Cassani, E.; Doria, Enrico; Nielsen, Erik

doi:10.2135/cropsci2004.0651

Cited by 68 publications

(80 citation statements)

References 37 publications

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“…As independently found for both lpa1-1 and lpa1-241 alleles, mips1S gene expression was reduced in developing seeds (Pilu et al, 2003;Shukla et al, 2004), but in both alleles no molecular lesions in the gene coding region were found (Shukla et al, 2004;Pilu et al, 2005).…”

Section: Introductionsupporting

confidence: 70%

“…Although biochemical, mapping and gene expression data suggest the mips1s gene as a candidate for lpa1 mutation in maize (Raboy et al, 2000;Pilu et al, 2003;Shukla et al, 2004;Pilu et al, 2005), recent transposon mutagenesis experiments found that a novel gene designated ZmMRP4 (accession number EF86878), coding a multidrug resistance-associated-protein (MRP) mapping near the mips1s sequence, is the actual responsible gene for lpa1 mutation (Shi et al, 2007). MRP proteins represent a subfamily of ATP-binding cassette transmembrane transporters widespread in all eukaryotes, which in plants are involved in several functions, such as xenobiotic detoxification, organic ions transport, oxidative stress tolerance (Swarbreck et al, 2003) and even transpiration control (Klein et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, it has been shown that negative pleiotropic effects lead to lethality in those individuals showing less than 20% of the wild-type phytate amount (Pilu et al, 2005). Genetic data concerning the heredity of the lpa1-241 trait suggest that an epigenetic phenomenon called paramutation might be involved in this trait (Pilu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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A paramutation phenomenon is involved in the genetics of maize low phytic acid1-241 (lpa1-241) trait

et al. 2008

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So far, in maize, three classes of mutants involved in phytic acid biosynthesis have been isolated: lpa1, lpa2 and lpa3. In 2007, a gene tagging experiment performed by Shi et al. found that mutations in ZmMRP4 (multidrug resistance-associated proteins 4) gene cause lpa1 phenotype. In previous studies, we isolated and described a single recessive lpa mutation (originally named lpa241), which was allelic to the lpa1-1 mutant, and was consequently renamed lpa1-241. It showed a decrease in the expression of the myo-inositol (Ins)-3-phosphate synthase gene (mips1S). In this study, we present genetic and molecular analyses of the lpa1-241 mutation that indicate an epigenetic origin of this trait, that is, a paramutagenic interaction that results in meiotically heritable changes in ZmMRP4 gene expression, causing a strong pleiotropic effect on the whole plant. The use of a 5-Azacytidine treatment provided data suggesting an association between gene methylation and the lpa1-241 phenotype. To our knowledge, this is the first report of a paramutagenic activity not involving flavonoid biosynthesis in maize, but regarding a key enzyme of an important metabolic pathway in plants.

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Section: Introductionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A paramutation phenomenon is involved in the genetics of maize low phytic acid1-241 (lpa1-241) trait

et al. 2008

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“…8 Low-phytate maize mutants 9 can improve iron bioavailability, 10,11 but have low yields and other negative effects. 12,13 Although natural variability in maize iron bioavailability 14 may provide a path to breeding improved varieties, transgenic biofortification could provide another route. Grains have been engineered for improved iron in a variety of ways.…”

Section: ■ Introductionmentioning

confidence: 99%

Iron Bioavailability of Maize Hemoglobin in a Caco-2 Cell Culture Model

Bodnar

Proulx

Scott

et al. 2013

J. Agric. Food Chem.

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Maize (Zea mays) is an important staple crop in many parts of the world but has low iron bioavailability, in part due to its high phytate content. Hemoglobin is a form of iron that is highly bioavailable, and its bioavailability is not inhibited by phytate. It was hypothesized that maize hemoglobin is a highly bioavailable iron source and that biofortification of maize with iron can be accomplished by overexpression of maize globin in the endosperm. Maize was transformed with a gene construct encoding a translational fusion of maize globin and green fluorescent protein under transcriptional control of the maize 27 kDa γ-zein promoter. Iron bioavailability of maize hemoglobin produced in Escherichia coli and of stably transformed seeds expressing the maize globin−GFP fusion was determined using an in vitro Caco-2 cell culture model. Maize flour fortified with maize hemoglobin was found to have iron bioavailability that is not significantly different from that of flour fortified with ferrous sulfate or bovine hemoglobin but is significantly higher than unfortified flour. Transformed maize grain expressing maize globin was found to have iron bioavailability similar to that of untransformed seeds. These results suggest that maize globin produced in E. coli may be an effective iron fortificant, but overexpressing maize globin in maize endosperm may require a different strategy to increase bioavailable iron content in maize KeywordsInterdepartmental Genetics Graduate Program, biofortification, hemoglobin, iron bioavailability, transgenic maize ABSTRACT: Maize (Zea mays) is an important staple crop in many parts of the world but has low iron bioavailability, in part due to its high phytate content. Hemoglobin is a form of iron that is highly bioavailable, and its bioavailability is not inhibited by phytate. It was hypothesized that maize hemoglobin is a highly bioavailable iron source and that biofortification of maize with iron can be accomplished by overexpression of maize globin in the endosperm. Maize was transformed with a gene construct encoding a translational fusion of maize globin and green fluorescent protein under transcriptional control of the maize 27 kDa γ-zein promoter. Iron bioavailability of maize hemoglobin produced in Escherichia coli and of stably transformed seeds expressing the maize globin−GFP fusion was determined using an in vitro Caco-2 cell culture model. Maize flour fortified with maize hemoglobin was found to have iron bioavailability that is not significantly different from that of flour fortified with ferrous sulfate or bovine hemoglobin but is significantly higher than unfortified flour. Transformed maize grain expressing maize globin was found to have iron bioavailability similar to that of untransformed seeds. These results suggest that maize globin produced in E. coli may be an effective iron fortificant, but overexpressing maize globin in maize endosperm may require a different strategy to increase bioavailable iron content in maize.

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“…Similarly, antisense transgenic lines of MIPS genes in Solanum tuberosum plants showed altered plant morphology (Keller et al 1998). All low phytic acid (lpa) mutants have been shown to be associated with negative effects in terms of seed physiology and plant performance, such as compromised germination and emergence, stress tolerance, and seed filling (Meis et al 2003;Pilu et al 2005;Bregitzer and Raboy 2006;Guttieri et al 2006). More recently, Campion et al (2009) isolated a P. vulgaris lpa mutant line (lpa-280-10) obtained by the chemical mutagenesis (ethyl methanesulfonate) of a lectine-free bean line showing reduced phytic acid, raffinosaccharides and iron cations in the seed compared with the wild type.…”

Section: Introductionmentioning

confidence: 99%

Comparative Expression and Cellular Localization of Myo-inositol Phosphate Synthase (MIPS) in the Wild Type and in an EMS Mutant During Common Bean (Phaseolus vulgaris L.) Seed Development

et al. 2011

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In plants, the myo-inositol-1-phosphate synthase (EC 5.5.1.4; MIPS) is an evolutionarily conserved enzyme protein and catalyzes the synthesis of myo-inositol, which is a central molecule required for cell metabolism and plant growth. A full-length cDNA encoding common bean (Phaseolus vulgaris L.) MIPS (designated Pv_BAT93 MIPS) was isolated from seed of P. vulgaris (cv, BAT93) by rapid amplification of cDNA ends. The cDNA of Pv_BAT93 MIPS comprised 1,873 bp, which encodes 510 amino acids. The Pv_BAT93 MIPS gene was highly homologous with those of other plant species, such as P. vulgaris (cv, Taylor's Horticultural), Arabidopsis thaliana, Medicago sativa and Glycine max. DNA blot analysis indicated that at least three copies of MIPS are present in the common bean genome. RT-PCR analysis indicated that the Pv_BAT93 MIPS transcripts existed in developing seeds and other common bean tissues, including leaves, flowers, and cotyledons but showed lower levels expression in roots and stems. Real-time quantitative PCR showed that Pv_BAT93 MIPS gene is differentially expressed during common bean seed development. In situ hybridization of developing seeds in the wild type and in the ethyl methanesulfonate mutant showed that expression of Pv_BAT93 MIPS was identified in the embryo tissues, from the globular to late cotyledon stages, confirming a possible involvement of Pv_BAT93 MIPS in common bean seed development.

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The Maize lpa241 Mutation Causes a Remarkable Variability of Expression and Some Pleiotropic Effects

Cited by 68 publications

References 37 publications

A paramutation phenomenon is involved in the genetics of maize low phytic acid1-241 (lpa1-241) trait

A paramutation phenomenon is involved in the genetics of maize low phytic acid1-241 (lpa1-241) trait

Iron Bioavailability of Maize Hemoglobin in a Caco-2 Cell Culture Model

Comparative Expression and Cellular Localization of Myo-inositol Phosphate Synthase (MIPS) in the Wild Type and in an EMS Mutant During Common Bean (Phaseolus vulgaris L.) Seed Development

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