The long noncoding RNA TP73‐AS1 promotes tumorigenicity of medulloblastoma cells

Abstract

Medulloblastoma is the most common malignant brain cancer in children. Since previous studies have mainly focused on alterations in the coding genome, our understanding of the contribution of long noncoding RNAs (lncRNAs) to medulloblastoma biology is just emerging. Using patient‐derived data, we show that the promoter of lncRNA TP73‐AS1 is hypomethylated and that the transcript is highly expressed in the SHH subgroup. Furthermore, high expression of TP73‐AS1 is correlated with poor outcome in patients with TP53 wild‐type SHH tumors. Silencing TP73‐AS1 in medulloblastoma tumor cells induced apoptosis, while proliferation and migration were inhibited in culture. In vivo, silencing TP73‐AS1 in medulloblastoma tumor cells resulted in reduced tumor growth, reduced proliferation of tumor cells, increased apoptosis and led to prolonged survival of tumor‐bearing mice. Together, our study suggests that the lncRNA TP73‐AS1 is a prognostic marker and therapeutic target in medulloblastoma tumors and serves as a proof of concept that lncRNAs are important factors in the disease.

LINC00294 negatively modulates cell proliferation in glioma through a neurofilament medium‐mediated pathway via interacting with miR‐1278

“…lncRNA TP73-AS1 possesses tumor promotive potential in medulloblastoma. 11 Besides, Linc00889 exerts oncogenic effect in spinal ependymomas. 12 Recently, the involvement of lncRNAs in glioma has also increasingly been reported.…”

Long non‑coding RNA TP73‑AS1 accelerates the progression and cisplatin resistance of non‑small cell lung cancer by upregulating the expression of TRIM29 via competitively targeting microRNA‑34a‑5p

“…altogether, these previous studies suggested that lncrnas played essential roles in the progression of nSclc. Tumor protein 73 aS1 (TP73-aS1) has been found to be involved in several types of cancer (11,12). However, the role of TP73-aS1 in the development of nSclc remains unclear.…”

Non-Coding RNAs in Brain Tumors, the Contribution of lncRNAs, circRNAs, and snoRNAs to Cancer Development—Their Diagnostic and Therapeutic Potential

“…There are also ncRNAs affecting cell cycle distribution in medulloblastoma. For instance, upregulated in MB, lncRNA TP73-AS1 affects apoptosis evasion and stimulates proliferation; however, the molecular mechanism behind its action still remains unclear [ 113 ]. The individual ncRNAs involved in regulation of cell cycle and apoptosis are presented in Table 1 .…”