The long non-coding RNA AK023948 enhances tumor progression in hepatocellular carcinoma

Ye, Bailiang; Hu, Bingren; Zheng, Zhenlin; Zheng, Rujie; Shi, Yixiong

doi:10.3892/etm.2017.5019

Cited by 13 publications

(7 citation statements)

References 30 publications

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“…The reaction was performed with incubation at 42°C for 1 h, and the enzyme was subsequently inactivated by incubation at 85°C for 5 min. The total reaction volume was 20 µl and the mixture was prepared as previously described (16). For qPCR, the PCR solution contained 1 ml cDNA, 1 ml primers and 10 ml SYBR Green and 5 ml RT-qPCR Master mix (all from Invitrogen; Thermo Fisher Scientific, Inc.).…”

Section: Methodsmentioning

confidence: 99%

Long noncoding RNA MAFG‑AS1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells through downregulation of miR‑6852

et al. 2019

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Long noncoding RNAs (lncRNAs) have been shown to participate in the development and progression of a number of different types of cancer, including hepatocellular carcinoma (HCC). A recent report has indicated that lncRNA MAFG-antisense 1 (AS1) promotes colorectal cancer. However, the role of MAFG-AS1 in other types of cancer remains unclear. The aim of the present study was to examine the effect of lncRNA MAFG-AS1 in HCC. Based on The Cancer Genome Atlas database and reverse transcription-quantitative PCR results, it was determined that lncRNA MAFG-AS1 expression was increased in HCC tissues and cell lines. Following knockdown of lncRNA MAFG-AS1, a Cell Counting Kit-8 assay and Transwell assay demonstrated that the proliferation, migration and invasion of HCC cell lines were significantly inhibited. It was additionally demonstrated that there was a negative regulatory association between lncRNA MAFG-AS1 and miR-6852. Inhibition of miR-6852 increased proliferation, migration and invasion of HCC cell lines. LncRNA MAFG-AS1 promoted HCC development by dampening miR-6852 function and may thus be a novel target for treating patients with HCC.

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Section: Methodsmentioning

confidence: 99%

Long noncoding RNA MAFG‑AS1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells through downregulation of miR‑6852

et al. 2019

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“…The lncRNAs are involved in a variety of biological processes, including proliferation, differentiation, autophagy, and apoptosis 8–10. In the field of cancer research, lncRNAs have been found to regulate the progression of various types of cancers such as glioma,11 breast cancer,12 colorectal cancer,13 liver cancer,14 and leukemia15 and are associated with recurrence and poor prognosis. A large number of dysregulated lncRNAs have been identified in gastric cancer till now 16.…”

Section: Introductionmentioning

confidence: 99%

<p>The lncRNA ZEB2-AS1 is upregulated in gastric cancer and affects cell proliferation and invasion via miR-143-5p/HIF-1α axis</p>

Wu¹,

Gao²,

Liu³

et al. 2019

OTT

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BackgroundGrowing evidence has implicated the important role of the long non-coding RNAs (lncRNAs) in gastric cancer progression. In this study, we examined the expression of lncRNA zinc finger E-box-binding homeobox 2 antisense RNA 1 (ZEB2-AS1) in gastric cancer tissues and elucidated the molecular mechanisms underlying ZEB2-AS1-mediated gastric cancer progression.MethodsQuantitative real-time PCR measured the gene expression level; CCK-8, colony formation and cell invasion assays determined gastric cancer cell proliferation, growth and invasion, respectively; the xenograft nude mice model was used to determine in vivo tumor growth; Bioinformatics analysis and luciferase reporter assay determined the downstream targets of ZEB2-AS1 and miR-143-5p. The expression of ZEB2-AS1 was upregulated in gastric cancer cell lines.ResultsKnockdown of ZEB2-AS1 suppressed gastric cancer cell proliferation, growth and invasion, and also suppressed in vivo tumor growth in the nude mice. Overexpression of ZEB2-AS1 potentiated gastric cancer cell proliferation, growth and invasion. Bioinformatics analysis and luciferase reporter assay showed that miR-143-5p was a direct target of ZEB2-AS1 and was negatively regulated by ZEB2-AS1. Furthermore, hypoxia-inducible factor-1α (HIF-1α) was found to be a target of miR-143-5p and was negatively regulated by miR-143-5p. The rescue in vitro assays showed that the effects of ZEB2-AS1 overexpression on gastric cancer cell proliferation, growth and invasion was mediated via miR-143-5p/HIF-1α. ZEB2-AS1 and HIF-1α was upregulated in gastric cancer tissues, while miR-143-5p was down-regulated; and ZEB2-AS1 expression level was inversely correlated with miR-143-5p expression level, and positively correlated with HIF-1α mRNA expression level; while miR-143-5p expression level was inversely correlated with HIF-1α expression level. High ZEB2-AS1 expression level was correlated with poor differentiation, lymph node metastasis and distant metastasis.ConclusionCollectively, our results indicated that ZEB2-AS1 was up-regulated in gastric cancer tissues and cells and promoted cell proliferation and metastasis through miR-143-5p/HIF-1α pathway, which may provide a promising target for treatment of gastric cancer.

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“… 8 – 10 Therefore, it is urgent to explore new regulating mechanisms for HCC progression to identify more therapeutic targets for HCC treatment. 11 – 13 …”

Section: Introductionmentioning

confidence: 99%

Downregulation of lncRNA OGFRP1 inhibits hepatocellular carcinoma progression by AKT/mTOR and Wnt/β-catenin signaling pathways

Chen¹,

You²,

Zheng³

et al. 2018

CMAR

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IntroductionIncreasing evidence demonstrates that long noncoding RNAs (lncRNAs) play important roles in the progression of hepatocellular carcinoma (HCC) by regulating gene expression. However, the identification of functional lncRNAs in HCC remains insufficient. Our study aimed to investigate the function of lncRNA OGFRP1, which has not been functionally researched before, in Hep3B and HepG2 cells.MethodslncRNA OGFRP1 in HCC cells was down-regulated by using RNAi technology. Quantitative real-time polymerase chain reaction was used to determine the mRNA expression of lncRNA OGFRP1. Cell proliferation was examined by CCK8 and clone formation assays. Cell cycle and apoptosis were analyzed by flow cytometry. Cell migration and invasion were assessed by using Scratch assay and transwell assay, respectively. Protein expression of signaling pathways was determined by using Western blot.ResultsCell proliferation of Hep3B was significantly inhibited by down-regulation of lncRNA OGFRP1 (P<0.05). Moreover, siOGFRP1 transfection induced Hep3B cell cycle arrest and apoptosis by regulating the expression of related proteins. Cell migration and invasion of Hep3B were also significantly inhibited by down-regulation of lncRNA OGFRP1. Wnt/β-catenin signaling pathway, involved in epithelial–mesenchymal transition (EMT), was inactivated by lncRNA OGFRP1 downregulation, including decreased expression of Wnt3a, β-catenin, N-cadherin and vimentin and increased expression of E-cadherin. We also found that the inhibitory effect of lncRNA OGFRP1 knockdown on Hep3B was mediated by the AKT/mTOR signaling pathway and IGF-1, an AKT signaling activator, could rescue the cellular phenotype. However, knockdown of lncRNA OGFRP1 did not influence cell proliferation, migration and invasion in HepG2 cells.ConclusionWe found that downregulation of lncRNA OGFRP1 suppressed the proliferation and EMT of HCC Hep3B cells through AKT and Wnt/β-catenin signaling pathways. However, lncRNA OGFRP1 exhibited a differentiated function in different HCC cell lines, which required further study in the future.

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The long non-coding RNA AK023948 enhances tumor progression in hepatocellular carcinoma

Cited by 13 publications

References 30 publications

Long noncoding RNA MAFG‑AS1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells through downregulation of miR‑6852

Long noncoding RNA MAFG‑AS1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells through downregulation of miR‑6852

<p>The lncRNA ZEB2-AS1 is upregulated in gastric cancer and affects cell proliferation and invasion via miR-143-5p/HIF-1α axis</p>

Downregulation of lncRNA OGFRP1 inhibits hepatocellular carcinoma progression by AKT/mTOR and Wnt/β-catenin signaling pathways

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The long non-coding RNA AK023948 enhances tumor progression in hepatocellular carcinoma

Cited by 13 publications

References 30 publications

Long noncoding RNA MAFG‑AS1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells through downregulation of miR‑6852

Long noncoding RNA MAFG‑AS1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells through downregulation of miR‑6852

<p>The lncRNA ZEB2-AS1 is upregulated in gastric cancer and affects cell proliferation and invasion via miR-143-5p/HIF-1&alpha; axis</p>

Downregulation of lncRNA OGFRP1 inhibits hepatocellular carcinoma progression by AKT/mTOR and Wnt/&beta;-catenin signaling pathways

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<p>The lncRNA ZEB2-AS1 is upregulated in gastric cancer and affects cell proliferation and invasion via miR-143-5p/HIF-1α axis</p>

Downregulation of lncRNA OGFRP1 inhibits hepatocellular carcinoma progression by AKT/mTOR and Wnt/β-catenin signaling pathways