The Level of Secreted Laccase Activity in the Edible Fungi and their Growing Cycles are Closely Related

Abstract

This article focuses on the relation between laccase-secreting ability and growing cycle in the edible fungi. First, laccase activities of fifteen different edible fungi were detected and determined by plate assay and spectrophotometric method using 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) as the substrate. The results showed the laccase-secreting ability in the edible fungi and their growing cycles are closely related. The edible fungi strains with short growing cycles originate from their high levels of secreted laccase activity. However, those strains require long growing cycles due to the low levels of secreted laccase, even no detectable laccase activity. The research provides the first evidence on the corresponding relation between the level of secreted laccase activity and growth cycles of edible fungi. Our study has significantly increased the understanding of the role of laccase in the growth and development of edible fungi.

The multigene family of fungal laccases and their expression in the white rot basidiomycete Flammulina velutipes

“…Laccases are directly involved in lignin bioconversion in A. bisporus and L. edodes (Ohga et al, 1999;Ohga and Roys, 2001); The levels of laccases secreted by edible fungi are also closely related to production cycles, with heightened laccase secretion levels in fungi with short growth cycles (Sun et al, 2011). Laccase activities and production can be induced by various factors during fungal growth (Chen et al, 2003).…”

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

“…Indeed, laccase has been developed as a novel screening marker in mushroom breeding (Sun et al, 2014). The levels of secreted laccase activity in edible mushrooms and their growing cycles are closely related, and short growing cycles are accompanied by high laccase activity (Sun et al, 2011). …”

The relationship between lignin peroxidase and manganese peroxidase production capacities and cultivation periods of mushrooms

“…Group II had middle level of cultivation periods, and group III had the longest cultivation periods among all groups. The strains in group I, group II or group III also showed different cultivation periods (Pang et al ., 2003; Zhang, 2003; Sun et al ., 2011). The results showed that LiP-secreting and MnP-secreting capacity and variety of mushroom are closely related to cultivation period.…”

“…a−: colour of mixture has no change; +, ++, +++: colour of mixture is changed more and more quickly.bReferences [1], [2] and [3] are Sun et al ., 2011, Zhang, 2003 and Pang et al ., 2003 respectively, and the cultivation periods refer to the industrial production.ACCC, Agricultural Culture Collection of China; LDFs, litter-degrading fungi; WRFs, white-rot fungi.…”

“…Therefore, lignin can be used as the components of mushrooms, and the level of secreted laccase, LiP and MnP activities in the mushrooms would affect the growing cycles of mushrooms. Previous reports show that the mushroom strains with high level of secreted laccase activity have short growing cycles (Sun et al ., 2011; Xu et al ., 2012). And some reports indicate that fungal laccase partake detoxification of phenolic compounds and sporophore development (Bollag et al ., 1988; Zhao and Kwan, 1999; Ohga and Royse, 2001).…”