The Laboratory Swine

Bollen, Peter; Hansen, Axel Kornerup; Alstrup, Aage Kristian Olsen

doi:10.1201/9780849376283

Cited by 32 publications

(54 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To our knowledge, no studies to date have investigated effects of GM maize on organ and intestinal health indicators in pigs throughout their entire productive life. Pigs are an excellent model for humans when considering similarities in digestive, liver and kidney anatomy and physiology (Bollen et al, 2000;Lewis and Southern, 2001;Swindle, 2007). Therefore, in addition to providing additional insight into possible physiological alterations in response to feeding GM maize to pigs, pig feeding studies may also provide data relevant to human nutrition.…”

Section: Introductionmentioning

confidence: 99%

Effect of feeding genetically modified Bt MON810 maize to ∼40-day-old pigs for 110 days on growth and health indicators

Buzoianu

Walsh

Rea

et al. 2012

animal

View full text Add to dashboard Cite

A total of 72 male weaned pigs were used in a 110-day study to investigate the effect of feeding genetically modified (GM) Bt MON810 maize on selected growth and health indicators. It was hypothesised that in pigs fed Bt maize, growth and health are not impacted compared with pigs fed isogenic maize-based diets. Following a 12-day basal period, pigs (10.7 ± 1.9 kg body weight (BW); ∼40 days old) were blocked by weight and ancestry and randomly assigned to treatments: (1) non-GM maize diet for 110 days (non-GM), (2) GM maize diet for 110 days (GM), (3) non-GM maize diet for 30 days followed by GM maize diet up to day 110 (non-GM/GM) and (4) GM maize diet for 30 days followed by non-GM maize diet up to day 110 (GM/non-GM). BW and daily feed intake were recorded on days 0, 30, 60 and 110 (n= 15). Body composition was determined by dual energy X-ray absorptiometry (n= 10) on day 80. Following slaughter on day 110, organs and intestines were weighed and sampled for histological analysis and urine was collected for biochemical analysis (n= 10). Serum biochemistry analysis was performed on days 0, 30, 60, 100 and 110. Growth performance and serum biochemistry were analysed as repeated measures with time and treatment as main factors. Thesliceoption of SAS was used to determine treatment differences at individual time points. There was no effect of feeding GM maize on overall growth, body composition, organ and intestinal weight and histology or serum biochemistry on days 60 and 100 and on urine biochemistry on day 110. A treatment × time interaction was observed for serum urea (SU;P< 0.05), creatinine (SC;P< 0.05) and aspartate aminotransferase (AST;P< 0.05). On day 30, SU was lower for the non-GM/GM treatment compared with the non-GM, GM and GM/non-GM treatments (P< 0.05). On day 110, SC was higher for the non-GM/GM and GM/non-GM treatments compared with non-GM and GM treatments (P< 0.05). Overall, serum total protein was lower for the GM/non-GM treatment compared with the non-GM/GM treatment (P< 0.05). The magnitude of change observed in some serum biochemical parameters did not indicate organ dysfunction and the changes were not accompanied by histological lesions. Long-term feeding of GM maize to pigs did not adversely affect growth or the selected health indicators investigated.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of feeding genetically modified Bt MON810 maize to ∼40-day-old pigs for 110 days on growth and health indicators

Buzoianu

Walsh

Rea

et al. 2012

animal

View full text Add to dashboard Cite

show abstract

“…The amount of food distributed to the control group was based on the nutritional recommendation for mini-pigs. 27 In this study, the first group was referred to as OF (for overfeeding) and the second was referred to as NF (normal feeding).…”

Section: Experimental Designmentioning

confidence: 99%

“…A group of mini-pigs were overfed (1.5 times the recommended caloric intake for pigs 27 ) a western-type diet, rich in saturated fats (50% of total fats) and in high glycaemic index carbohydrates. In the second group, which served as a control, mini-pigs were fed a recommended human-type diet, rich in monounsaturated fats (50% of total fats) and in low glycaemic index carbohydrates.…”

Section: Experimental Designmentioning

confidence: 99%

Childhood obesity and insulin resistance in a Yucatan mini-piglet model: putative roles of IGF-1 and muscle PPARs in adipose tissue activity and development

et al. 2005

View full text Add to dashboard Cite

OBJECTIVE:To explore metabolic and cellular modifications induced during childhood obesity, in a novel animal model of obese mini-piglets. DESIGN: A total of 10 four-month old Yucatan mini-pigs were followed from prepuberty to adulthood. Animals were divided into two groups. The first one had been overfed (OF) a western-type diet and the second one had been normally fed a control recommended human-type diet (NF). MEASUREMENTS: Plasma insulin-like growth factor 1 (IGF-1), insulin, leptin, nonesterified fatty acids, triglycerides (TGs) and glucose were determined at sexual maturity and at young adulthood. Quantitative gene expressions of peroxysome-proliferatoractivated receptors (PPARs), glucose transporter 4, insulin receptor, IGF-1, leptin and interleukin-6 (IL-6) in skeletal muscle, adipose tissue and liver were also measured at both stages. Adult insulin sensitivity was measured via euglycaemichyperinsulinaemic clamps. RESULTS: Increased body weight in adult OF pigs was associated with increased body size and low insulin sensitivity. Sexually mature OF pigs had higher IGF-1 plasma concentrations than their lean littermates (Po0.05). In the OF group, TGs and glucose were both decreased (Po0.05). Muscle PPARg and a in OF pubescent pigs as compared to NF pigs were 11 times higher and 20 times lower, respectively (Po0.01). CONCLUSION: Obesity and insulin resistance induced by overfeeding mini-pigs during development and puberty were not associated with the cluster of metabolic modifications frequently observed in their adult littermates. Increased IGF-1 concentrations and modifications of skeletal muscle PPAR (a and g) expressions may help the young obese pig to partially regulate its glycaemia and triglyceridaemia through an increase of fat mass, which maintains its high insulin sensitivity.

show abstract

“…A number of cloned pigs have been produced, including those with genes for GFP [1][2][3][4][5][6][7][8], as well as alpha1,3-galactosyltransferase knockout pigs [9][10][11][12][13][14][15][16]. In this manner, genetically modified pigs are being used in a wide variety of biomedical fields, ranging from basic research to organ transplantation.To date, more than ten breeds of miniature pigs have been established as experimental and companion animals [17]. As far as the use of pigs as experimental animals is concerned, miniature pigs are smaller and easier to handle than common domestic pigs, and they have been used in a variety of fields, such as medical and pharmacological research [10,[18][19][20][21][22][23][24][25].…”

mentioning

confidence: 99%

Production of Transgenic and Non-Transgenic Clones in Miniature Pigs by Somatic Cell Nuclear Transfer

et al. 2008

View full text Add to dashboard Cite

Abstract. Miniature pigs have been recognized as valuable experimental animals in various fields such as medical and pharmaceutical research. However, the amount of information on somatic cell cloning in miniature pigs, as well as genetically modified miniature pigs, is much less than that available for common domestic pigs. The objective of the present study was to establish an efficient technique of cloning miniature pigs by somatic cell nuclear transfer. A high pregnancy rate was achieved following transfer of parthenogenetic (3/3) and cloned (5/6) embryos using female miniature pigs in the early pregnancy period as recipients after estrus synchronization with prostaglandin F2 alpha analog and gonadotrophins. The production efficiency of the cloned miniature pigs using male and female fetal fibroblasts as nucleus donors was 0.9% (2/215 and 3/331, respectively). Cloned miniature pigs were also produced efficiently (7.8%, 5/64) by transferring reconstructed embryos into the uteri of common domestic pigs. When donor cells transfected with the green fluorescent protein (GFP) gene were used in nuclear transfer, the production efficiency of the reconstructed embryos and rate of blastocyst development were comparable to those obtained by non-transfected cells. When transfected cell-derived reconstructed embryos were transferred to three common domestic pig recipients, all became pregnant, and a total of ten transgenic cloned miniature pigs were obtained (piglet production efficiency: 2.7%, 10/365). Hence, we were able to establish a practical system for producing cloned and transgenic-cloned miniature pigs with a syngeneic background. Key words: Embryo transfer, In vitro matured oocyte, Miniature pig, Nuclear transfer, Transgenic (J. Reprod. Dev. 54: [156][157][158][159][160][161][162][163] 2008) n recent years, techniques for producing cloned pigs by somatic cell nuclear transfer (SCNT) have been actively utilized to produce genetically modified pigs. A number of cloned pigs have been produced, including those with genes for GFP [1][2][3][4][5][6][7][8], as well as alpha1,3-galactosyltransferase knockout pigs [9][10][11][12][13][14][15][16]. In this manner, genetically modified pigs are being used in a wide variety of biomedical fields, ranging from basic research to organ transplantation.To date, more than ten breeds of miniature pigs have been established as experimental and companion animals [17]. As far as the use of pigs as experimental animals is concerned, miniature pigs are smaller and easier to handle than common domestic pigs, and they have been used in a variety of fields, such as medical and pharmacological research [10,[18][19][20][21][22][23][24][25]. However, compared with common domestic pigs, far less information regarding the production of cloned and genetically modified pigs is available for miniature pigs [10,13,14,[26][27][28][29][30][31].In the present study, we conducted a series of experiments including (i) validation of an estrus synchronization procedure for miniature pig recipients, (i...

show abstract

The Laboratory Swine

Cited by 32 publications

References 0 publications

Effect of feeding genetically modified Bt MON810 maize to ∼40-day-old pigs for 110 days on growth and health indicators

Effect of feeding genetically modified Bt MON810 maize to ∼40-day-old pigs for 110 days on growth and health indicators

Childhood obesity and insulin resistance in a Yucatan mini-piglet model: putative roles of IGF-1 and muscle PPARs in adipose tissue activity and development

Production of Transgenic and Non-Transgenic Clones in Miniature Pigs by Somatic Cell Nuclear Transfer

Contact Info

Product

Resources

About