The karyotype of Trypanosoma cruzi Dm 28c: Comparison with other T. cruzi strains and trypanosomatids

Aymerich, Stéphane; Goldenberg, Samuel

doi:10.1016/0014-4894(89)90178-1

Cited by 28 publications

(16 citation statements)

References 24 publications

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“…However, the pattern of lectin-binding sites obtained for T. cruzi Dm28c (isolated from opossum) differed from that obtained for the Y strain (isolated from man) (Pereira et al, 1980). This observation is in agreement with the generally described heterogeneity of the parasite (Morel et al, 1980;Dvorak et al, 1980;Engel et al, 1982;Schottelius & Uhlenbruck, 1983;Tibayrenc et al, 1986;Stevens et al, 1988. Aymerich & Goldenberg, 1989).…”

Section: Discussionsupporting

confidence: 92%

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Andrade¹,

Esteves²,

Angluster³

et al. 1991

Journal of General Microbiology

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Highly purified lectins with specificities for receptor molecules containing sialic acid, N-acetylglucosamine (DGlcNAc), N-acetylgalactosamine (D-GalNAc), galactose (D-Gal), mannose-like residues (D-Man) or L-fucose (LFuc), were used to determine changes in cell-surface carbohydrates of the protozoal parasite Trypanosomu cruzi during metacyclogenesis under chemically defined conditions. Of the D-GalNAc-binding lectins, BS-I selectively agglutinated metacyclic trypomastigotes, MPL was selective for replicating epimastigotes, whereas SBA strongly agglutinated all developmental stages of T. cruzi. WGA (sialic acid and/or D-GIcNAc specific) was also reactive with differentiating epimastigotes and metacyclic trypomastigotes but displayed a higher reactivity with replicating epimastigote forms. A progressive decrease in agglutinating activity was observed for jacaline (specific for D-Gal) during the metacyclogenesis process; conversely, a progressive increase in affinity was observed for RCA-I (D-Gabspecific), although the reactivity of other D-Gal-specific lectins (PNA and AxP) was strong at all developmental stages. All developmental stages of T. cruzi were agglutinated by Con A and Lens cufinaris lectins (specific for D-Man-like residues); however, they were unreactive with the L-fucose-binding lectins from Lotus tetrugonolobos and Ufex europueus. These agglutination assays were further confirmed by binding studies using I z51-labelled lectins. Neuraminidase activity was detected in supernatants of cell-free differentiation medium using the PNA hemagglutination test with human A erythrocytes. The most pronounced differences in lectin agglutination activity were observed between replicating and differentiating epimastigotes, suggesting that changes in the composition of accessible cell-surface carbohydrates precede the morphological transformation of epimastigotes into metacyclic trypomastigotes.

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Section: Discussionsupporting

confidence: 92%

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Andrade¹,

Esteves²,

Angluster³

et al. 1991

Journal of General Microbiology

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“…Correlation between the variation at the nucleotide sequence level and the changes in pathophysiological properties were reported in other trypanosome species Webster 1987, Aymerich andGoldenberg 1989). As previously reported, T. evansi exhibits a widespread geographic distribution, a variety of host species infected, and an array of clinical symptoms and pathogenicity, hence suggesting genetic diversity within T. evansi species (Luckins 1988, Uche et al 1992.…”

Section: Introductionsupporting

confidence: 57%

Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines

Villareal

Mingala

Rivera³

2013

Acta Parasitologica

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Trypanosoma evansi infection in the Philippines is frequently reported to affect the country's livestock, particularly, the buffaloes. To assess the prevalence and intraspecific diversity of T. evansi in the country, blood samples from water buffaloes in different geographical regions were collected during an outbreak. T. evansi was detected in all 79 animals tested using PCR targeting the RoTat 1.2 VSG gene. Sequencing of the rDNA complete internal transcribed spacer (ITS) region including the 5.8S subunit showed high similarity (99-100%) between Philippine isolates and known T. evansi isolates in Genbank. Tree construction based on the same region confirmed the close relationship between Philippine and reported Thai isolates as compared to Egyptian isolates separated by relatively small genetic distances, 47 polymorphisms, despite the clustering in four branches. Overall, the results of this study prove genetic diversity within T. evansi species despite previous reports on limited heterogeneity among isolates worldwide.

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“…Previous studies on isoenzymes (Miles et al 1977, Bogliolo et al 1986, Tibayrenc et al 1986, total DNA content (Borst et al 1982), molecular karyotypes and restriction fragment length polymorphisms (Gibson & Miles 1986, Aymerich & Goldenberg 1986, Henriksson et al 1990, Dietrich et al 1990) and more recently microsatellites studies (Oliveira et al 1998) are in agreement with diploidy in T. cruzi and also support that the genome of this parasite is remarkably plastic. Despite the genetic diversity and diploidy of T. cruzi, it has been proposed that this parasite has a clonal population structure and asexual reproduction, this is supported by the great deviation from Hardy-Weinberg equilibrium observed in natural populations (Tibayrenc et al 1990).…”

mentioning

confidence: 65%

Characterization of Trypanosoma cruzi

Murta

Romanha

1999

Mem. Inst. Oswaldo Cruz

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The karyotype of Trypanosoma cruzi Dm 28c: Comparison with other T. cruzi strains and trypanosomatids

Cited by 28 publications

References 24 publications

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines

Characterization of Trypanosoma cruzi

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