The isolation of peste des petits ruminants virus from Northern India

Nanda, Y.P.; Chatterjee, Amaresh; Purohit, A. N.; Diallo, Adama; Innui, K.; Sharma, Rakesh; Libeau, Geneviève; Thevasagayam, J.A.; Brüning, A; Kitching, R.; Anderson, J.; Barrett, Thomas; Taylor, W. P.

doi:10.1016/0378-1135(96)00025-9

Cited by 130 publications

(89 citation statements)

References 8 publications

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“…It is well established that PPR disease is more severe in goats than in sheep [26,31,33,35,38]. In controlled studies [15,26], some North Indian goat isolates of PPRV were shown to cause severe disease in goats as compared to sheep. In addition, at least one goat isolate required serial passage to even induce clinical signs in sheep (Dr. Muthuchelvan, Indian Veterinary Research Institute, personal communication).…”

Section: Discussionmentioning

confidence: 99%

Comparative immunogenicity of two peste des petitis ruminants (PPR) vaccines in South Indian sheep and goats under field conditions

et al. 2013

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Peste des petitis ruminants (PPR) is an economically important endemic viral disease of sheep and goats in India, where several different homologous PPR vaccine candidates have been developed. We evaluated the serological response to two vaccine strains, Arasur/87 and Sungri/96, in South Indian cross-bred and native sheep and goats reared under organized and unorganized settings. Animals seronegative (percent inhibition or PI \40) by competitive enzyme-linked immunosorbent assay (c-ELISA) were immunized with either of the vaccine strains or placebo. Sera collected on 21, 60 and 90 days post-vaccination were subjected to c-ELISA and serum neutralization test (SNT). Seropositivity (PI [40), seroconversion (fourfold increase in SNT titres) and seroprotection (SNT titre of C8 deemed to be protective) ranged from 66.7 to 84.0 %, 56.0 to 69.2 %, and 60.0 to 76.0 %, respectively. However, no significant difference was observed between responses to the two vaccine strains. These results support the premise that the two vaccine strains are equally efficacious.

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Section: Discussionmentioning

confidence: 99%

Comparative immunogenicity of two peste des petitis ruminants (PPR) vaccines in South Indian sheep and goats under field conditions

et al. 2013

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“…Until severe epidemics swept through the rest of India in 1994 and onwards the disease was thought to be restricted in Southern India. Since then the disease became enzootic in many northern states of India [92,95] causing huge economic losses to the small ruminant industry. In Maharashtra, the first PPR outbreak in goats was reported in 1994 [72].…”

Section: Geographical Distributionmentioning

confidence: 99%

“…The clinical features or signs are better described under experimental condition rather than the natural infection. Both in natural as well experimental PPR infection, the incubation period ranged from 2 to 6 days [74,75,95,153]. In experimentally infected kids, the first clinical sign of pyrexia (40.6-44.2°C) was observed on days 5, followed by diarrhea (between day 5 and 8) and death within 10 days [143].…”

Section: Diagnosismentioning

confidence: 99%

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

130

118

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Peste des petits ruminants (PPR) is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality and caused by PPR virus. PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and particularly severely affects poor farmer's economy. The disease is clinically manifested by pyrexia, oculo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhoea and bronchopneumonia. The disease can be diagnosed from its clinical signs, pathological lesions, and specific detection of virus antigen/antibodies/genome in the clinical samples by various serological tests and molecular assays. PPR is the one of the priority animal diseases whose control is considered important for poverty alleviation in enzootic countries. Availability of effective and safe live attenuated cell culture PPR vaccines and diagnostics have boosted the recently launched centrally sponsored control programme in India and also in other countries. This review article primarily focus on the current scenario of PPR diagnosis and its control programme with advancement of research areas that have taken place in the recent years with future perspectives.

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“…The cells were propagated in a growth medium containing Eagle's minimum essential medium (EMEM) supplemented with 10% fetal bovine serum (FCS; Sigma-Aldrich, St. Louis, USA). The isolation of PPR virus from tissue and blood samples was carried out using protocol given by Nanda et al (1996) with little modification. After two blind passages, virus was isolated and confirmed by PPR sandwich ELISA.…”

Section: Methodsmentioning

confidence: 99%

“…Diagnosis of PPR virus (PPRV) infection relies on laboratory techniques such as virus isolation, demonstration of PPRV antigen, and nucleic acid. Definitive identification of PPR virus isolates can be made by immunological techniques like enzyme immunoassay (Nanda et al 1996) and molecular techniques like reverse transcription polymerase chain reaction (RT-PCR) (Ozkul et al 2002). Virulence and biological properties of the virus can be detected by titration method (Mariner et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of peste des petits ruminants virus tropism in Vero and Vero/SLAM cells

Sannat

Rajak

Singh

et al. 2014

Journal of Applied Animal Research

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A comparative study was conducted to investigate tropism of peste des petits ruminants (PPR) virus in Vero and Vero/ SLAM (signaling lymphocyte activation molecule) cells. PPR virus was isolated from tissues and blood samples of sheep and goats following second passage in both the cell line. Intensive cytopathic effects and higher antigen load (by sandwich enzyme linked immuno sorbant assay [ELISA]) were observed in Vero/SLAM cells as compared to Vero cells. A significantly higher virus titer (i.e. ranging from 4.5 to 6.5 log 10 TCID 50 /ml) was detected in Vero/SLAM than Vero cell isolates (i.e. ranging from 3.5 to 4.5 log 10 TCID 50 /ml). The present study has demonstrated that Vero cells expressing the SLAM receptors are highly efficient for isolating PPR virus (PPRV) from pathological samples, as it offers a substantial improvement including faster viral replication resulting in primary viral isolation in a shorter period of time, and higher yield of virus than traditional cell culture methodologies.

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The isolation of peste des petits ruminants virus from Northern India

Cited by 130 publications

References 8 publications

Comparative immunogenicity of two peste des petitis ruminants (PPR) vaccines in South Indian sheep and goats under field conditions

Comparative immunogenicity of two peste des petitis ruminants (PPR) vaccines in South Indian sheep and goats under field conditions

Diagnosis and control of peste des petits ruminants: a comprehensive review

Comparative analysis of peste des petits ruminants virus tropism in Vero and Vero/SLAM cells

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