The Isolation and Selected Properties of Blood Monocytes

Bennett, William E.; Cohn, Zanvil A.

doi:10.1084/jem.123.1.145

Cited by 282 publications

(99 citation statements)

References 23 publications

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“…Such observations have been confirmed repeatedly (4,5) and extended to document increases in cell size, protein content, lysosomal and ectoenzyme activity, surface receptor expression, and phagocytic capacity (4,(6)(7)(8)(9)(10)(11)(12). This differentiation sequence in vitro closely parallels that observed when monocytes emigrate from the vasculature into the tissues (13)(14)(15).…”

Section: Introductionmentioning

confidence: 65%

Hydrogen peroxide metabolism in human monocytes during differentiation in vitro.

Nakagawara¹,

Nathan²,

Cohn³

1981

J. Clin. Invest.

330

161

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A B S T R A C T The capacity of human blood monocytes to secrete hydrogen peroxide (H202) and superoxide (O-) was measured as the cells differentiated during 4 wk of culture. Morphologic transformation of monocytes into macrophages, epithelioid cells, and multinucleated giant cells accompanied a steady increase in the content of protein per cell, from 0.77 mg/ 107 cells on days 0 to 11.77 mg/107 cells on days 20 to 29. In contrast, secretion of H202 by adherent monocytes was 859±73 nmol/60 min per mg protein (mean ±SEM, n = 18) on day 0, rose 40% on day 3, and then fell rapidly, remaining below 6% of the initial values after day 10. The decline in capacity to secrete reactive oxygen intermediates was observed whether H202 or O-were measured, whether the cells were challenged with phorbol myristate acetate or with opsonized zymosan, and whether the results were expressed per milligram cell protein or per cell.Superoxide dismutase activity tripled in adherent monocytes from day 0 to day 3, and thereafter remained elevated through at least day 16. In contrast, the activity of myeloperoxidase declined rapidly, catalase and glutathione peroxidase declined more gradually, and glutathione reductase and glutathione remained constant throughout the period of observation. Thus, the decline in capacity to secrete H202 could not be attributed to increases in cellular levels of these antioxidants.On the first day of culture, H202 release was enhanced up to fourfold by inclusion of sodium azide or potassium cyanide in the assay medium. This enhancement appeared to be due to inhibition of monocyte A preliminary report of this study was presented at the

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Section: Introductionmentioning

confidence: 65%

Hydrogen peroxide metabolism in human monocytes during differentiation in vitro.

Nakagawara¹,

Nathan²,

Cohn³

1981

J. Clin. Invest.

330

161

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“…The albumin gradient, a modification of the method of Bennett and Cohn (22), yielded 15% more monocytes than did Ficoll-Hypaque but no significant differences were noted in either cell morphology or in vitro cell survival. This monocyte yield (74%) equalled the maximum previously reported for other albumin gradients (30-75%) (10,11) and exceeds the estimated 50% yield by the method of Einstein et al (6).…”

Section: Effect Of Latex Ingestion On Monocyte 5'-nucleotidasementioning

confidence: 99%

The separation, long-term cultivation, and maturation of the human monocyte.

Johnson¹,

Mei²,

Cohn³

1977

The Journal of Experimental Medicine

284

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“…The process of the in vitro transformation of blood monocytes to mature macrophages has been extensively studied by different investigators using mainly monocytes from animal sources (2,16,33,35), and increases in lysosomal enzyme activities have been reported. Similar in vitro changes have also been described in cultured mouse peritoneal macrophages (16).…”

Section: Tlme After R E M O V a L O F 3 5~~ (Hrs)mentioning

confidence: 99%

Monocyte-derived Macrophages: An in Vitro System for Studying Hereditary Lysosomal Storage Diseases

Yatziv

Epstein

1978

Pediatr Res

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SummaryMature macrophages obtained by the growth and differentiation of blood monocvtes have been used to develo~ an in vitro svstem for the study of hereditary human lysosom~l storage diseases. Maturation of monocytes into macrophages is rapid, and the maximal activities of the lysosomal hydrolases, P-galactosidase, P-glucuronidase, a-mannosidase, and P-N-acetyl glucosaminidase, is reached by the seventh day in culture. The rate of 35S04 incorporation into mucopolysaccharides is also maximal at 7 days. Normal macrophages attain a steady state of 35S04 labeling within 5 hr of culture and degrade about 60% of sulfated mucopolysaccharides in 5 hr. By contrast, macrophages from patients with the Hunter syndrome (MPS 11), when grown in MPS I1 serum, degrade only 19% of sulfated MPS within 12 hr and incorporate more 35S04 into MPS. Significant correction of the metabolic defects is achieved by incubation of the Hunter syndrome macrophages in normal serum, demonstrating that the abnormal MPS I1 macrophages are responsive to exogenously supplied enzyme (a-L-iduronate sulfatase). Preliminary studies of MPS turnover in macrophages from two obligate MPS heterozygotes are indicative of partially impaired M P S degradation, with increased 35S04 incorporation and a lower rate of 35S04-MPS degradation.

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The Isolation and Selected Properties of Blood Monocytes

Cited by 282 publications

References 23 publications

Hydrogen peroxide metabolism in human monocytes during differentiation in vitro.

Hydrogen peroxide metabolism in human monocytes during differentiation in vitro.

The separation, long-term cultivation, and maturation of the human monocyte.

Monocyte-derived Macrophages: An in Vitro System for Studying Hereditary Lysosomal Storage Diseases

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