The investigation of cytogenetic and oxidative effects of diffractaic acid on human lymphocyte cultures

Demir, Leyla; Toğar, Başak; Türkez, Hasan; Sozio, Piera; Aslan, Ali; Stefano, Antonio Di

doi:10.1590/s1516-8913201502752

Cited by 9 publications

(4 citation statements)

References 38 publications

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“…TOS of diffractaic acid, a lichen secondary metabolite was examined in human blood tissue and determined that this metabolite did not significantly increase TOS level. 34 Furthermore, it was pronounced that in the treatments performed in cultured human lymphocytes, many lichens such as Xanthoria elegans , 35 Bryoria capillaris , and Peltigera rufescens 36 might use as therapeutic agent by not creating oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of antioxidant and cytotoxic effects of olivetoric and physodic acid in cultured human amnion fibroblasts

et al. 2016

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It is known that lichens are utilized for the treatment of many diseases including ulcer, diabetes, and cancer for many years. Secondary metabolites in the structure of the lichens provide various activity properties for them. In the present study, cytotoxic and oxidative effects of main constituents of Pseudevernia furfuracea (L.) Zopf (Parmeliaceae), olivetoric acid (OA), and physodic acid (PA) were investigated on cultured human amnion fibroblasts (HAFs). OA and PA were isolated from P. furfuracea using column chromatography and their structures were determined by proton nuclear magnetic resonance and carbon-13 nuclear magnetic resonance. HAFs were incubated during 48 h in the presence of OA and PA, at different concentrations from 6.25 mg/L to 200 mg/L. OA showed higher cytotoxicity than PA. In fact, median inhibitory concentration values of OA and PA were 571.27 and 3373.69 mg/L, respectively. The lower concentrations (<50 mg/L) of OA and PA did not cause oxidative stress and genotoxicity; furthermore, they supported anti-oxidative capacity of HAFs. Therefore, all these data suggested that both tested metabolites, especially PA might be developed as natural health medicine to protect human body against oxidative stress and genotoxicity. As far as we know, this is the first report on the cytotoxic and anti-oxidative activities of OA and PA on HAFs.

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Section: Discussionmentioning

confidence: 99%

Evaluation of antioxidant and cytotoxic effects of olivetoric and physodic acid in cultured human amnion fibroblasts

et al. 2016

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“…Lichens, symbiotic organisms, have common usage due to its therapeutic features in several diseases which contains both algae and fungal [7]. Usnea longisima whose antioxidant and anti-ulcer activities was studied by our research group was investigated in treatment of broken bone, leg lesions and wards [8].…”

Section: Issn: 2399-9098mentioning

confidence: 99%

The effect of usnic acid supplementation on rabbit’s tissues surrounding implant apoptosis and some enzyme activities

Odabaşoğlu¹,

Yıldırım²,

Halıcı³

et al. 2019

Glob Drugs Therap

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Usnea longissima Ach., is a type of lichen known for its protective effects. In this study, we examined the effects of olive oil and usnic acid against apoptosis in rabbit femurs after titanium implantation. We determined that lichen metabolite and olive oil activate caspase-dependent programmatic cell death unlike necrosis. Both orally and locally administered olive oil and usnic acid showed proapoptotic effect through the activation of caspase 2, 3, 8 and 9. Also, they showed strong myeloperoxidase (MPO) and inducible nitric oxide synthase (iNOS) activities. However, iNOS and MPO activities do not have a strong effect in local application. Furthermore, usnic acid and olive oil decreased SOD activity and GSH level. In parallel, titanium implantation in these tissues also decreased. In the light of the data obtained, both usnic acid and olive oil induced apoptotic cell death. Therefore, it can be used as a proapoptotic agent in the treatment of various diseases.Odabasoglu F (2019) The effect of usnic acid supplementation on rabbit's tissues surrounding implant apoptosis and some enzyme activities

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“…Bununla ilgili, Demir vd. [41] [44]. Bu kapsamda, antioksidan kapasitesinin varlığı defalarca çalışılmış olan önemli liken metaboliti usnik asittir.…”

Section: Tartışma Ve Sonuçunclassified

<i>Platismatia glauca</i> (L.) W.L.Culb. & C.F.Culb.'nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri

Emsen¹,

Aslan²,

Kaya³

2017

SDÜ Fen Bil Enst Der

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Özet: Likenler yapısında farklı metabolitleri bulunduran ve bu bileşenleri sayesinde antik çağlardan beri birçok hastalığın tedavisinde yararlanılan organizma olarak dikkat çekmektedir. Bu yüzden, mevcut çalışma önemli liken türlerinden biri olan Platismatia glauca (L.) W.L.Culb. & C.F.Culb.'nın insan lenfositleri üzerindeki etkilerini incelemeyi amaçlamıştır. P. glauca'dan elde edilen metanol (PME) ve su (PSE) ekstraktlarının hücreler üzerindeki sitotoksik aktiviteleri 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür ve laktat dehidrogenaz testleri ile analiz edilmiştir. PME ve PSE ile muamele edilen lenfositlerdeki oksidatif değişimin belirlenmesi amacıyla toplam antioksidan kapasite (TAK) ve toplam oksidan durum (TOD) analizleri gerçekleştirilmiştir. Bu uygulamalara ek olarak, ekstraktların hücreler üzerinde sebep olduğu genetik hasar hücrelerdeki 8-hidroksi-2′deoksiguanozin seviyeleri ölçülerek tespit edilmiştir. Hesaplanan IC50 değerleri, PSE'nin (162,26 mg/L) PME'ye (84,02 mg/L) kıyasla çok daha düşük seviyede sitotoksik aktivite gösterdiğini ortaya çıkarmıştır. Her iki ekstraktın tüm konsantrasyonlarının (6,25-200 mg/L) negatif kontrole kıyasla istatistiksel olarak (p>0,05) hücrelerdeki TAK'yi yükselttiği ve aynı zamanda düşük konsantrasyonlarının genetik hasar meydana getirmediği belirlenmiştir. Tüm uygulamalar göz önüne alındığında, PME ve özellikle PSE'nin belli konsantrasyonlarının (<25 mg/L) lenfositler üzerinde anlamlı derecede oksidatif ve genetik hasara neden olmadan TAK'yi yükselttiği ve bu nedenle P. glauca likeninin doğal bir antioksidan olarak kullanılabileceği gözlenmiştir.

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The investigation of cytogenetic and oxidative effects of diffractaic acid on human lymphocyte cultures

Abstract: Diffractaic acid (DA) is a naturally occurring depside derivative found in several lichen

Cited by 9 publications

References 38 publications

Evaluation of antioxidant and cytotoxic effects of olivetoric and physodic acid in cultured human amnion fibroblasts

Evaluation of antioxidant and cytotoxic effects of olivetoric and physodic acid in cultured human amnion fibroblasts

The effect of usnic acid supplementation on rabbit’s tissues surrounding implant apoptosis and some enzyme activities

<i>Platismatia glauca</i> (L.) W.L.Culb. & C.F.Culb.'nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri

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