1999
DOI: 10.1016/s0014-5793(98)01694-9
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Abstract: Recently the signal transduction function for oxidative phosphorylation was found to be second order in ADP

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Cited by 127 publications
(116 citation statements)
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References 25 publications
(28 reference statements)
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“…Additionally, it has been shown that the COX-IV is a key regulatory subunit of the mammalian CcO. It inhibits the catalytic activity of the enzyme allosterically by binding of ATP to the matrix domain (68), suggesting that CcO is able to sense the cellular energy level, such as the ATP:ADP ratio (69,70). On the basis of our data, it is possible that expression of COX-IV-2 provides U251 cells with a mechanism to switch from oxidative phosphorylation to an anaerobic ATP production.…”
Section: Discussionmentioning
confidence: 99%
“…Additionally, it has been shown that the COX-IV is a key regulatory subunit of the mammalian CcO. It inhibits the catalytic activity of the enzyme allosterically by binding of ATP to the matrix domain (68), suggesting that CcO is able to sense the cellular energy level, such as the ATP:ADP ratio (69,70). On the basis of our data, it is possible that expression of COX-IV-2 provides U251 cells with a mechanism to switch from oxidative phosphorylation to an anaerobic ATP production.…”
Section: Discussionmentioning
confidence: 99%
“…An increase in oxidized DAB, which is directly proportional to the CcO activity, was measured at 450 nm. In the second approach, CcO was solubilized from SMP with 1.5% sodium cholate and reconstituted into asolectin-cardiolipin vesicles as described (24,25). Orientation of CcO in the vesicles was determined by spectral analysis following sequential reduction with ascorbate and dithionate (26).…”
Section: Methodsmentioning
confidence: 99%
“…9. They are apparently not related to the allosteric ATP-inhibition (21,30), which appears to involve phosphorylation of subunit I (see Figs. 6 and 8).…”
Section: Fig 2 Western Blots and Coomassie Blue Staining (Cb) Ofmentioning
confidence: 99%
“…Mitochondrial respiration, and thus CcO activity, is also inhibited at high ATP/ADP ratios through high ⌬⌿ m values, known as "respiratory control" (27,28). The allosteric ATP-inhibition represents a second mechanism of respiratory control (29), which is independent of ⌬⌿ m (30). The allosteric ATP-inhibition, however, is lost when the enzyme is dephosphorylated (31,32), possibly at Ser-441 of bovine heart CcO subunit I (23).…”
mentioning
confidence: 99%