The interaction of some spin-labeled sulfonamides with bovine erythrocyte carbonic anhydrase B

Chignell, Colin F.; Starkweather, Donnas K.; Erlich, Ronald H.

doi:10.1016/0005-2795(72)90127-4

Cited by 26 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2) and resembled the rigid glass, powder or polycrystalline spectrum of the nitroxide radical [7]. This observation indicates that the nitroxide group of sulfonamide I is highly immobilized when this drug binds to the active site of BCA-B [3,7].…”

Section: Resultsmentioning

confidence: 79%

“…The large central peak in figure 2 con tains contributions from both unbound and bound sulfonamide I. Since peak B in figure 2 contains little or no contribution from the broad spec trum of the highly immobilized enzyme-bound sulfonamide I [3], the amplitude of this peak can serve as a measure of free sulfonamide I in the system. The association constant of sulfonamide I for BCA-B was esti mated from measurements of the amplitude of peak B to be 6.25 x 10° M_1.…”

Section: Resultsmentioning

confidence: 99%

“…1). However, in the presence of BCA-B, the ESR spectrum of sulfonamide I became broad and asymmetric [3] (fig. 2) and resembled the rigid glass, powder or polycrystalline spectrum of the nitroxide radical [7].…”

Section: Resultsmentioning

confidence: 99%

“…Chignell and co-workers have recently syn thesized a series of spin-labeled aromatic sulfonamides containing the nitroxide-free radical [4] and studied their interaction with bovine ery throcyte carbonic anhydrase B [3,5] and human erythrocyte carbonic anhydrase B and C [6]. In this report we show how a spin-labeled sul fonamide (I) can be used to measure the concentration of certain diuretic sulfonamides in body fluids.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Novel Assay for Erythrocyte Carbonic Anhydrase and Certain Sulfonamide Drugs Employing Spin-Labels

Chignell¹,

Starkweather²

1972

Pharmacology

View full text Add to dashboard Cite

A novel assay procedure has been developed for the determination of sulfonamide drugs with the general formula ArSO₂NH₂ where Ar = aryl (either homocyclic or heterocyclic). The assay depends on the displacement of a spin-labeled sulfonamide from the active site of bovine carbonic anhydrase B and can detect concentrations of sulfonamide as low as 0.3 µg/ml. This technique can also be used to assay low concentrations of carbonic anhydrase in hemolysates of red blood cells without prior removal of the hemoglobin or isolation of the enzyme.

show abstract

Section: Resultsmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Novel Assay for Erythrocyte Carbonic Anhydrase and Certain Sulfonamide Drugs Employing Spin-Labels

Chignell¹,

Starkweather²

1972

Pharmacology

View full text Add to dashboard Cite

show abstract

“…The distance corresponding to this break has been interpreted as the minimum depth of the cleft [13,14]. The essential sulfhydryl group of chicken liver Fru-Pzase is therefore most likely located in a cleft-like environment of 10.5 A in depth.…”

Section: Modification Of the Enzyme By Nitroxidecontaining Iodoacetammentioning

confidence: 99%

Spin label studies of the essential sulfhydryl group environment in chicken liver fructose‐1,6‐bisphosphatase

Zeidan¹,

Han²,

Johnson³

1985

FEBS Letters

View full text Add to dashboard Cite

The local environment of the essential sulfhydryl groups in chicken liver fructose-1,6-bisphosphatase has been investigated by ESR techniques using a series of iodoacetamide spin labels, varying in chain length between the iodoacetate and nitroxide free radical group. The ESR spectrum of spin-labeled chicken liver fructose-l ,6-bisphosphatase showed that the sites of labeling were highly immobilized when the enzyme was chemically modified by spin label iodoacetate, suggesting that the sulfhydryl groups of the protein are in a small, confined environment. From the change in the ESR spectra of these nitroxides as a function of chain length, we conclude that the sulfhydryl group is located in a cleft approx. 10.5 A in depth. Spin label ESR Essential suljhydryl group Chicken liverFructose-I,&bisphosphatase

show abstract

The interaction of some spin-labeled sulfonamides with bovine erythrocyte carbonic anhydrase B

Cited by 26 publications

References 13 publications

A Novel Assay for Erythrocyte Carbonic Anhydrase and Certain Sulfonamide Drugs Employing Spin-Labels

A Novel Assay for Erythrocyte Carbonic Anhydrase and Certain Sulfonamide Drugs Employing Spin-Labels

Spin label studies of the essential sulfhydryl group environment in chicken liver fructose‐1,6‐bisphosphatase

Drug‐Biomolecule Interactions: Topographical Study of Active Site of Erythrocyte Carbonic Anhydrase Using Spin‐Labeled Sulfanilamide Drugs

Contact Info

Product

Resources

About