The interaction of high copper and zinc doses in acid soil changes the physiological state and development of the root system in young grapevines (Vitis vinifera)

Tiecher, Tadeu Luís; Soriani, Hilda Hildebrand; Tiecher, Tales; Ceretta, Carlos Alberto; Nicoloso, Fernando Teixeira; Tarouco, Camila Peligrinotti; Clasen, Bárbara; Conti, Lessandro De; Tassinari, Adriele; Melo, G. W. B. de; Brunetto, Gustavo

doi:10.1016/j.ecoenv.2017.11.074

Cited by 37 publications

(24 citation statements)

References 64 publications

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“…Cell division and elongation processes are often inhibited in plants grown in Cu-contaminated environments(Dey et al, 2014;Tiecher et al, 2018). The inhibition of these processes was evident in both plants investigated in the current study (Figures2 a, b, c and Figures 3 a, b, c).…”

supporting

confidence: 56%

“…The increased Cu concentration in plant roots also results from intracellular production of organic acids such as citrate (Keller et al, 2015;Murphy et al, 1999), as well as of phytochelatins acting in cytosol. During the metal ion chelation process, chelated metals are sequestered and compartmentalized in the vacuole (Mourato et al, 2015) to avoid detrimental effects on cell metabolism (Jan and Parray, 2016;Tiecher et al, 2018). The preferential Cu accumulation in the root system is essential to prevent excessive Cu contents in the shoot, mainly in leaves.…”

Section: Discussionmentioning

confidence: 99%

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Physiological Responses of Beet and Cabbage Plants Exposed to Copper and their Potential Insertion in Human Food Chain

Schmitt¹,

Andriolo²,

Silva³

et al. 2021

Preprint

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Copper (Cu) can be toxic to vegetables when it is absorbed and accumulated at large concentrations, a fact that increases the risk of excessive addition of this metal to the human food chain. The aims of the current study are (1) to determine the Cu concentrations that have critical toxic effects on beet and cabbage plants, and the potential of these plants to enter the human food chain; as well as (2) to assess the physiological and biochemical responses of representatives of these vegetables grown in nutrient solution presenting increasing Cu concentrations. Beet and cabbage plants were grown for 75 days in pots filled with sand added with nutrient solution presenting six Cu concentrations: 0.00, 0.52, 1.02, 1.52, 2.02 and 2.52 mg Cu L -1 . Dry matter yield and Cu accumulation in different plant organs were evaluated. Photosynthetic pigment contents, lipid peroxidation levels (TBARs), superoxide dismutase (SOD, EC 1.15.1.1) and peroxidase (POD, EC 1.11.1.7) activity, and hydrogen peroxide (H2O2 ) concentrations in leaves were evaluated. Critical Cu concentrations that led to toxicity in plant organs such as beetroot and cabbage head, which are often found in human diets, corresponded to 1.43 mg Cu L -1 and 1.59 mg Cu L -1 , respectively. High Cu concentrations in the nutrient solution have increased Cu concentrations and accumulation in plant tissues. This outcome justified the increased POD and SOD enzyme activity in the leaves of beet and cabbage plants, respectively, as well as was the cause of reduced plant growth in both crops. Cabbage plants presented higher tolerance to increased Cu levels in the growing environment than beet plants. However, it is necessary being careful at the time to consume both vegetables, when they are grown in Cu-enriched environments.

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confidence: 56%

Section: Discussionmentioning

confidence: 99%

Physiological Responses of Beet and Cabbage Plants Exposed to Copper and their Potential Insertion in Human Food Chain

Schmitt¹,

Andriolo²,

Silva³

et al. 2021

Preprint

Self Cite

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“…Then, the kinetics of chlorophyll fluorescence induction and P700 oxidation were recorded simultaneously from the instrument. The light-adapted photosynthetic parameters were recorded after exposure to different light intensity (1445,1105,819,592,418,206,69,36,13 μmol photons m − 2 s − 1 ) for 240 s. The chlorophyll fluorescence parameters Fv/Fm, Y(II), Y(NPQ) and Y(NO) were calculated as described in Huang et al [44]. P700 parameters were measured and calculated as described in Yuan et al [45] and Huang et al [46].…”

Section: Measurement Of Gas Exchange Chlorophyll Fluorescence and P7mentioning

confidence: 99%

“…A whole genome survey shows that 4-10% of all sequenced proteins from prokaryotes and eukaryotes contain Zn-binding domains [8]. Zn plays an important role in decreasing the harmful effects of abiotic stresses through scavenging ROS, retaining heavy metal in roots and keeping heavy metal concentration in mesophyll cells in non-toxic forms [9][10][11][12][13]. These damage symptoms initially appear in young leaves and meristems of plants due to the low mobility of Zn [14].…”

Section: Introductionmentioning

confidence: 99%

Interaction of zinc and IAA alleviate aluminum-induced damage on photosystems via promoting proton motive force and reducing proton gradient in alfalfa

Xie

Wen

et al. 2020

BMC Plant Biol

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Background In acidic soils, aluminum (Al) competing with Zn results in Zn deficiency in plants. Zn is essential for auxin biosynthesis. Zn-mediated alleviation of Al toxicity has been rarely studied, the mechanism of Zn alleviation on Al-induced photoinhibition in photosystems remains unclear. The objective of this study was to investigate the effects of Zn and IAA on photosystems of Al-stressed alfalfa. Alfalfa seedlings with or without apical buds were exposed to 0 or100 μM AlCl3 combined with 0 or 50 μM ZnCl2, and then foliar spray with water or 6 mg L− 1 IAA. Results Our results showed that Al stress significantly decreased plant growth rate, net photosynthetic rate (Pn), quantum yields and electron transfer rates of PSI and PSII. Exogenous application of Zn and IAA significantly alleviated the Al-induced negative effects on photosynthetic machinery, and an interaction of Zn and IAA played an important role in the alleviative effects. After removing apical buds of Al-stressed alfalfa seedlings, the values of pmf, gH+ and Y(II) under exogenous spraying IAA were significantly higher, and ΔpHpmf was significantly lower in Zn addition than Al treatment alone, but the changes did not occur under none spraying IAA. The interaction of Zn and IAA directly increased Y(I), Y(II), ETRI and ETRII, and decreased O2− content of Al-stressed seedlings. In addition, the transcriptome analysis showed that fourteen functionally noted genes classified into functional category of energy production and conversion were differentially expressed in leaves of alfalfa seedlings with and without apical buds. Conclusion Our results suggest that the interaction of zinc and IAA alleviate aluminum-induced damage on photosystems via increasing pmf and decreasing ΔpHpmf between lumen and stroma.

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“…Traditionally, metal concentrations in plants’ organs, as well as the subsequent inhibition effects of biomass and elongation, have widely been considered the endpoints of chronic phytotoxicity in terrestrial ecosystems. Several studies have been conducted to determine the concentration–growth inhibition relationship in grapevines exposed to excess Cu [ 6 , 10 , 11 ]. In addition to physiological responses, it is recognized that heavy metals may cause molecular damage to plants through reactive oxygen species (ROS) formation.…”

Section: Introductionmentioning

confidence: 99%

Modeling Alleviative Effects of Ca, Mg, and K on Cu-Induced Oxidative Stress in Grapevine Roots Grown Hydroponically

Juang

Lo²,

Chen

2021

Molecules

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The aim of this study was to determine the pattern of alleviation effects of calcium (Ca), magnesium (Mg), and potassium (K) on copper (Cu)-induced oxidative toxicity in grapevine roots. Root growth, Cu and cation accumulation, reactive oxygen species (ROS) production, and antioxidant activities were examined in grapevine roots grown in nutrient solutions. The experimental setting was divided into three sets; each set contained a check (Hoagland solution only) and four treatments of simultaneous exposure to 15 μM Cu with four cation levels (i.e., Ca set: 0.5, 2.5, 5, and 10 mM Ca; Mg set: 0.2, 2, 4, and 8 mM Mg; K set: 0.6, 2.4, 4.8, and 9.6 mM K). A damage assessment model (DAM)-based approach was then developed to construct the dose-effect relationship between cation levels and the alleviation effects on Cu-induced oxidative stress. Model parameterization was performed by fitting the model to the experimental data using a nonlinear regression estimation. All data were analyzed by a one-way analysis of variance (ANOVA), followed by multiple comparisons using the least significant difference (LSD) test. The results showed that significant inhibitory effects on the elongation of roots occurred in grapevine roots treated with 15 μM Cu. The addition of Ca and Mg significantly mitigated phytotoxicity in root growth, whereas no significant effect of K treatment on root growth was found. With respect to oxidative stress, ROS and malondialdehyde (MDA) contents, as well as antioxidant (superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX)) activities, were stimulated in the roots after exposure to 15 μM Cu for three days. Moreover, H2O2 levels decreased significantly as Ca, Mg, and K concentrations increased, indicating that the coexistence of these cations effectively alleviated Cu-induced oxidative stress; however, alleviative effects were not observed in the assessment of the MDA content and antioxidant enzyme activities. Based on the DAM, an exponential decay equation was developed and successfully applied to characterize the alleviative effects of Ca, Mg, and K on the H2O2 content induced by Cu in the roots. In addition, compared with Mg and K, Ca was the most effective cation in the alleviation of Cu-induced ROS. Based on the results, it could be concluded that Cu inhibited root growth and Ca and Mg absorption in grapevines, and stimulated the production of ROS, lipid peroxidation, and antioxidant enzymes. Furthermore, the alleviation effects of cations on Cu-induced ROS were well described by the DAM-based approach developed in the present study.

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The interaction of high copper and zinc doses in acid soil changes the physiological state and development of the root system in young grapevines (Vitis vinifera)

Cited by 37 publications

References 64 publications

Physiological Responses of Beet and Cabbage Plants Exposed to Copper and their Potential Insertion in Human Food Chain

Physiological Responses of Beet and Cabbage Plants Exposed to Copper and their Potential Insertion in Human Food Chain

Interaction of zinc and IAA alleviate aluminum-induced damage on photosystems via promoting proton motive force and reducing proton gradient in alfalfa

Modeling Alleviative Effects of Ca, Mg, and K on Cu-Induced Oxidative Stress in Grapevine Roots Grown Hydroponically

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