The Interaction of 5-(Alkoxy)naphthalen-1-amine with Bovine Serum Albumin and Its Effect on the Conformation of Protein

Ojha, Bimlesh; Das, Gopal

doi:10.1021/jp907576r

Cited by 151 publications

(93 citation statements)

References 49 publications

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“…Therefore, it is unlikely that the conformation of HSA will be changed with the level of DMSO used in this study. 97 Milipore water was used in the preparation of buffer solution.…”

Section: Hsa Binding Experimentsmentioning

confidence: 99%

Quinoline based mono- and bis-(thio)carbohydrazones: synthesis, anticancer activity in 2D and 3D cancer and cancer stem cell models

Božić

Marinković

Bjelogrlić³

et al. 2016

RSC Adv.

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A comparative study of antitumor activity of mono-and bis-quinoline based (thio)carbohydrazones was investigated by a series of tests on two human malignant cell lines: acute monocytic leukemia (THP-1) and pancreatic adenocarcinoma cancer stem cells (AsPC-1). Thiocarbohydrazones (TCHs) revealed superior pro-apoptotic activity over carbohydrazones (CHs) on both tested cell phenotypes, also displaying multi-target profile activities. Programmed cell death triggered by TCHs was partially caspasedependent, mainly caspase-8 related. Activity against cancer stem cells (CSCs) was evaluated on 2D monolayers and 3D spheroidal models, where two out of three tested bis-TCHs successfully stimulated apoptosis accompanied by a reduction in size of treated spheres. Additionally, all bis-TCHs induced significant decrease in percentage of CD44-expressing AsPC-1 cells that indicate on their ability to induce reprogramming of CSC phenotype. Current results highly support further assessment of bisTCHs in order to specify their specific targets in cancer cells and particularly in the CSCs subpopulation.

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“…Therefore, it is unlikely that the conformation of HSA will be changed with the level of DMSO used in this study. 97 Milipore water was used in the preparation of buffer solution.…”

Section: Hsa Binding Experimentsmentioning

confidence: 99%

Quinoline based mono- and bis-(thio)carbohydrazones: synthesis, anticancer activity in 2D and 3D cancer and cancer stem cell models

Božić

Marinković

Bjelogrlić³

et al. 2016

RSC Adv.

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“…The fluorescence intensities of these residues decrease in the following order: Trp>Tyr>Phe [30]. Each BSA molecule has two Trp residues, one of which is located at the bottom of the hydrophobic pocket in domain II (Trp-212), while the other is in domain I (Trp-134) on the surface of the molecule [31][32][33]. Because the excited-state dipole moment of Trp is relatively large, the emission energy is highly sensitive to the polarity of the environment.…”

Section: Analysis Of the Aunps-bsa Interactionsmentioning

confidence: 99%

“…Conformational changes in BSA may disturb the microenvironment around the Trp residues and thus influence the fluorescence emission. Therefore, the Trp fluorescence is widely used to monitor conformational changes in proteins [31,34].…”

Section: Analysis Of the Aunps-bsa Interactionsmentioning

confidence: 99%

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

Shi

Xie

et al. 2012

Chin. Sci. Bull.

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The interactions between bovine serum albumin (BSA) and gold nanoparticles (AuNPs), and the conformational changes of BSA induced by this interaction, were investigated by UV-visible absorption spectroscopy, fluorescence spectroscopy, and Fourier transform infrared in combination with attenuated total reflection spectroscopy (ATR-FTIR). The critical adsorption density for preventing AuNP aggregation in 0.1 mol/L phosphate buffered saline (pH 7.2) was 23 BSA molecules per gold particle or 3.8×1012 BSA molecules/cm 2 . BSA bound to the AuNPs with high affinity (binding constant K s =7.59×10 8 L/mol), and the intrinsic fluorescence of BSA was quenched by the AuNPs in accordance with the static quenching mechanism. Both fluorescence spectroscopy and ATR-FTIR showed that AuNPs induced conformational changes in BSA, which resulted in it becoming less compact and increased the polarity of the microenvironment around the tryptophan residue Trp-212.

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“…The Gemini amphiphiles has possessed more interaction with BSA, as explained on the basis of higher value of K sv, as compared to single amphiphiles. The synchronous spectra and UV spectra of amphiphiles also show that the unfolding of proteins is mainly due to the interaction between amphiphiles and Trp residues of BSA [32]. TEM studies revealed that the mechanism of binding of single chain amphiphiles is different from that of Gemini amphiphiles.…”

Section: Resultsmentioning

confidence: 97%

Comparison of Physicochemical Studies of Single Tailed and Gemini Cyclohexanoxy Carbonyl Pyridinium Cationic Amphiphiles

Aggarwal¹

2017

J Proteomics Bioinform

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In the present research work, the physicochemical studies of surface active Cyclohexanoxy carbonyl Pyridinium single tailed and Gemini amphiphiles had been compared. Thermal stability, size of aggregates in aqueous system and interaction with a globular protein, Bovine Serum Albumin (BSA), was done by using TGA analysis, DLS, UV-visible, steady-state fluorescence and synchronous fluorescence and TEM respectively. On the basis of the experiments, it had been concluded that the Gemini amphiphile possessed less cytotoxicity, high thermally stability, form small sized compact aggregates and stable complex with BSA. The results have further been supported by higher value of Stern-Volmer quenching constant (K SV ) and a higher binding constants for Gemini amphiphiles as compared to single tailed amphiphiles. Moreover, on the basis of synchronous fluorescence spectra and UV spectra, it has been concluded that both the amphiphiles mainly interact with tryptophan residues. The less cytotoxicity values and stabilization of secondary structure of BSA in low concentration of ionic liquids implies that these ionic liquids can replaced the conventional surfactants in the detergent industries and used as a potential vehicles for the drug and gene delivery.the change in the wavelength of emission maximum (λ max ) and intensity parameters found to be sensitive to protein conformation and can be used to investigate the interactions between BSA and ionic liquids. DLS and TEM studies are used to see the change in conformation of the amphiphiles in the presence of BSA. Materials and Methods MaterialsGemini Amphiphiles (GA) and Single Tailed amphiphiles (ST) ( Figure 1) were synthesized in our lab by an earlier reported procedure [18,19]. Bovine Serum Albumin (BSA) (MW=66 kDa) was purchased from Central drug house (New Delhi, India) and was used as received. 0.01 M buffer, pH 7.2, was made from 0.01 M KH 2 PO 4 and 0.01 M sodium hydroxide. Potassium dihydrogen phosphate and sodium hydroxide were purchased from Merck, India. Millipore water was used in all experiments. Thermal gravimetric analysisThermal stability of both the amphiphiles had been investigated by Perkin Elmer Pyris 1 Thermal Gravimetric Analyzer (TGA). Both the samples were completely dried by rotatory vaccum pump. Both the samples were added in to the aluminum pans under the inert atmosphere at a heating rate of 10°C/min. Dynamic Light Scattering (DLS)The size of the studied cationic amphiphiles has been measured by the Zetasizer Nano ZS (Malvern Instrument Ltd.) UK. The inbuilt peltier device controls the temperature with an accuracy of ±0.1 K. The 1.0 mM aqueous solution of both amphiphiles has been filtered through 0.45 μM pore sized filter before measurement. CytotoxicityThe cytotoxicity of, 1.0 mM aqueous solution, both synthesized amphiphiles was carried out on C 6 glioma (cancerous brain cell line, passage number 65) by using MTT assay to calculate the IC 50 value of both the synthesized ionic liquids. Cells were seeded at a density of 10 × 10 3 cells/m...

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The Interaction of 5-(Alkoxy)naphthalen-1-amine with Bovine Serum Albumin and Its Effect on the Conformation of Protein

Cited by 151 publications

References 49 publications

Quinoline based mono- and bis-(thio)carbohydrazones: synthesis, anticancer activity in 2D and 3D cancer and cancer stem cell models

Quinoline based mono- and bis-(thio)carbohydrazones: synthesis, anticancer activity in 2D and 3D cancer and cancer stem cell models

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

Comparison of Physicochemical Studies of Single Tailed and Gemini Cyclohexanoxy Carbonyl Pyridinium Cationic Amphiphiles

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