The interaction between a combined knitted silk scaffold and microporous silk sponge with human mesenchymal stem cells for ligament tissue engineering

Liu, Haifeng; Fan, Hongbin; Wang, Yue; Toh, S.L.; Goh, James Cho‐Hong

doi:10.1016/j.biomaterials.2007.10.035

Cited by 192 publications

(136 citation statements)

References 31 publications

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“…62 However, this method may not provide the even and reproducible cell distribution needed to facilitate cell organization throughout the scaffold, as desired for most tissue-engineering applications, such as tissue-engineered vasculature, heart, tendon, and muscle. 9,[63][64][65] Because of the need for a more-efficient cell delivery method, dynamic seeding techniques were developed based on the principle that moving a cell solution throughout the construct would lead to high cell deposition in the scaffold porosity, as well as on the exterior surfaces. 12,66,67 Many investigations of dynamic seeding methods support the ability of dynamic seeding methods to more efficiently seed scaffolds.…”

mentioning

confidence: 99%

Method to Analyze Three-Dimensional Cell Distribution and Infiltration in Degradable Scaffolds

Thevenot

Nair

Dey

et al. 2008

Tissue Engineering Part C: Methods

149

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Effective cell seeding throughout the tissue scaffold often determines the success of tissue-engineering products, although most current methods focus on determining the total number, not the distribution, of the cells associated with tissue-engineering constructs. The purpose of this investigation was to establish a quick, convenient, and efficient method to quantify cell survival, distribution, and infiltration into degradable scaffolds using a combination of fluorescence cell staining and cryosectioning techniques. After cell seeding and culture for different periods of time, seeded scaffolds were stained with a live cell dye and then cryosectioned. Cryosectioned scaffolds were then recompiled into a three-dimensional (3D) image to visualize cell behavior after seeding. To test the effectiveness of this imaging method, four common seeding methods, including static surface seeding, cell injection, orbital shaker seeding, and centrifuge seeding, were investigated for their seeding efficacy. Using this new method, we were able to visualize the benefits and drawbacks of each seeding method with regard to the cell behavior in 3D within the scaffolds. This method is likely to provide useful information to assist the development of novel materials or cell-seeding methods for producing full-thickness tissue grafts.

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mentioning

confidence: 99%

Method to Analyze Three-Dimensional Cell Distribution and Infiltration in Degradable Scaffolds

Thevenot

Nair

Dey

et al. 2008

Tissue Engineering Part C: Methods

149

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“…Cells migrate throughout the body by attaching and detaching to the fibers of the ECM, pulling themselves along in the direction dictated by chemotactic signals. Connective tissues such as bone, ligament, and skin have denser and more highly organized ECM than tissues such as brain or adipose due to the increased demand for mechanical support, structure, and protection [1,[6][7][8][9][10][11][12][13][14][15][16][17].…”

Section: The Extracellular Matrixmentioning

confidence: 99%

A review of electrospinning manipulation techniques to direct fiber deposition and maximize pore size

et al. 2017

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Electrospinning has been widely accepted for several decades by the tissue engineering and regenerative medicine community as a technique for nanofiber production. Owing to the inherent flexibility of the electrospinning process, a number of techniques can be easily implemented to control fiber deposition (i.e. electric/magnetic field manipulation, use of alternating current, or air-based fiber focusing) and/or porosity (i.e. air impedance, sacrificial porogen/sacrificial fiber incorporation, cryo-electrospinning, or alternative techniques). The purpose of this review is to highlight some of the recent work using these techniques to create electrospun scaffolds appropriate for mimicking the structure of the native extracellular matrix, and to enhance the applicability of advanced electrospinning techniques in the field of tissue engineering.

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“…Silk-based scaffolds meet all of these requirements. Studies show that anterior cruciate ligament (ACL) FBs and mesenchymal stem cells grow well on silk scaffolds as well as combined knitted silk scaffolds and silk sponges for ligament tissue engineering [108,139,140]. While silk's versatility as a biomaterial and its controllable mechanical properties make it a strong candidate for ligament and tendon tissue engineering, much of its usage in this area has been in the form of woven or knitted constructs, with little literature on creating an electrospun SF ligament scaffold.…”

Section: Electrospinning Silk Fibroinmentioning

confidence: 99%

The Use of Natural Polymers in Tissue Engineering: A Focus on Electrospun Extracellular Matrix Analogues

et al. 2010

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Natural polymers such as collagens, elastin, and fibrinogen make up much of the body's native extracellular matrix (ECM). This ECM provides structure and mechanical integrity to tissues, as well as communicating with the cellular components it supports to help facilitate and regulate daily cellular processes and wound healing. An ideal tissue engineering scaffold would not only replicate the structure of this ECM, but would also replicate the many functions that the ECM performs. In the past decade, the process of electrospinning has proven effective in creating non-woven ECM analogue scaffolds of micro to nanoscale diameter fibers from an array of synthetic and natural polymers. The ability of this fabrication technique to utilize the aforementioned natural polymers to create tissue engineering scaffolds has yielded promising results, both in vitro and in vivo, due in part to the enhanced bioactivity afforded by materials normally found within the human body. This review will present the process of electrospinning and describe the use of natural polymers in the creation of bioactive ECM analogues in tissue engineering.

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The interaction between a combined knitted silk scaffold and microporous silk sponge with human mesenchymal stem cells for ligament tissue engineering

Cited by 192 publications

References 31 publications

Method to Analyze Three-Dimensional Cell Distribution and Infiltration in Degradable Scaffolds

Method to Analyze Three-Dimensional Cell Distribution and Infiltration in Degradable Scaffolds

A review of electrospinning manipulation techniques to direct fiber deposition and maximize pore size

The Use of Natural Polymers in Tissue Engineering: A Focus on Electrospun Extracellular Matrix Analogues

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