The inhibitory effect of the combination of two new peptides on biofilm formation by Acinetobacter baumannii

Irani, Nazanin; Basardeh, Eilnaz; Samiee, Fatemeh; Fateh, Abolfazl; Shooraj, Fahimeh; Rahimi, Ayoub; Shahcheraghi, Fereshteh; Vaziri, Farzam; Masoumi, Maryam; Pazhouhandeh, Mehrdad; Siadat, Seyed Davar; Kazemi‐Lomedasht, Fatemeh; Jamnani, Fatemeh Rahimi

doi:10.1016/j.micpath.2018.05.051

Cited by 18 publications

(22 citation statements)

References 44 publications

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“…Biofilms account for over 80% of microbial infections in the human body [ 52 ]. Bacterial cells in biofilms are 10–1000 times less susceptible to antimicrobial agents compared to their planktonic counterparts due to the physical impedance leading to poor diffusion of the drugs into the biofilm [ 53 ]. Also, bacteria embedded in a biofilm can evade the host immune system, therefore, contributing to its resistance mechanism [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation

Chipenzi

Baloyi

Mudondo

et al. 2020

Advances in Pharmacological and Pharmaceutical Sciences

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ESKAPE pathogens, namely, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species, are responsible for a majority of all healthcare-acquired infections (HAI). The bacteria cause nosocomial infections in immunocompromised patients. Extracts from Callistemon viminalis have been shown to have antibacterial, antifungal, and anti-inflammatory activities. Tormentic acid congener, a pentacyclic triterpene saponin, was isolated from C. viminalis leaves. This study aimed to investigate the antibacterial effects of tormentic acid congener and leaf extracts on biofilm formation by A. baumannii, S. aureus, S. pyogenes, and P. aeruginosa. The antibacterial effects were determined by the microbroth dilution method, and ciprofloxacin was used as the standard antibacterial drug. Biofilm formation and detachment assays were performed using crystal violet staining. Production of extracellular polymeric DNA and polysaccharides from biofilms was also determined. Tormentic acid congener showed time-dependent antibacterial activity against P. aeruginosa with a MIC of 100 µg/ml and caused significant protein leakage. Antibacterial activity was found when tormentic acid congener was tested against both S. aureus and P. aeruginosa. The MICs were found to be 25 µg/ml and 12.5 µg/ml for P. aeruginosa and S. aureus cells, respectively. S. pyogenes was found to be susceptible to tormentic acid congener and the hydroethanolic extract with an MIC of 100 µg/ml and 25 µg/ml, respectively. A. baumannii was found not to be susceptible to the compound or the extracts. The compound and the extracts caused a significant decrease in the biofilm extracellular polysaccharide content of S. pyogenes. The extracts and tormentic acid congener caused detachment of biofilms and decreased the release of extracellular DNA and capsular polysaccharides from biofilms of P. aeruginosa and S. aureus. Tormentic acid congener and extracts, thus, have significant antibacterial and antibiofilm activities on these selected ESKAPE bacteria and can act as source lead compounds for the development of antibacterial triterpenoids.

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Section: Resultsmentioning

confidence: 99%

An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation

Chipenzi

Baloyi

Mudondo

et al. 2020

Advances in Pharmacological and Pharmaceutical Sciences

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“…Considering the critical status of ESKAPE pathogens, several attempts have been made to find out AMP based effective therapeutics. To date, there are numerous natural as well as bioengineered AMPs reported to show in vitro (Björn et al, 2016; Cappiello et al, 2016; Liu et al, 2017; Gandt et al, 2018; Irani et al, 2018; Téllez et al, 2018) as well as in vivo (Björn et al, 2016; Liu et al, 2017) antimicrobial, antibiofilm, anti-inflammatory, and wound healing abilities with minimum cytotoxicity. Histatin 5, a natural histidine rich cationic human salivary peptide, is an example.…”

Section: Antimicrobial Peptides (Amps) In Therapymentioning

confidence: 99%

Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review

et al. 2019

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The acronym ESKAPE includes six nosocomial pathogens that exhibit multidrug resistance and virulence: Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa , and Enterobacter spp. Persistent use of antibiotics has provoked the emergence of multidrug resistant (MDR) and extensively drug resistant (XDR) bacteria, which render even the most effective drugs ineffective. Extended spectrum β-lactamase (ESBL) and carbapenemase producing Gram negative bacteria have emerged as an important therapeutic challenge. Development of novel therapeutics to treat drug resistant infections, especially those caused by ESKAPE pathogens is the need of the hour. Alternative therapies such as use of antibiotics in combination or with adjuvants, bacteriophages, antimicrobial peptides, nanoparticles, and photodynamic light therapy are widely reported. Many reviews published till date describe these therapies with respect to the various agents used, their dosage details and mechanism of action against MDR pathogens but very few have focused specifically on ESKAPE. The objective of this review is to describe the alternative therapies reported to treat ESKAPE infections, their advantages and limitations, potential application in vivo , and status in clinical trials. The review further highlights the importance of a combinatorial approach, wherein two or more therapies are used in combination in order to overcome their individual limitations, additional studies on which are warranted, before translating them into clinical practice. These advances could possibly give an alternate solution or extend the lifetime of current antimicrobials.

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“…The phage display technique is one of the approaches used to identify peptides with antibacterial properties [ 78 ]. This method was used to select peptides targeted to A. baumannii [ 79 ]. To search for antimicrobial peptides against A. baumannii growing either in planktonic or biofilm form, biopanning was performed using a peptide library on five XDR A. baumannii strains grown in a medium containing human blood (blood biopanning) and the biofilms formed by these strains (biofilm biopanning).…”

Section: Methodsmentioning

confidence: 99%

“…Among those, two peptides were selected based on the similarity of their amino acid composition to that of other known AMPs. Both peptides exhibited antibacterial activity against A. baumannii (MIC 500 μg/mL), as well as significant antimicrobial activity; the combination of these two peptides more effectively reduced the formation of a A. baumannii biofilm compared to each individual peptide [ 79 ].…”

Section: Methodsmentioning

confidence: 99%

Development of antimicrobial therapy methods to overcome the antibiotic resistance of Acinetobacter baumannii

Kisil

Ефименко

et al. 2020

Acta Naturae

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The spread of antibiotic resistance among pathogens represents a threat to human health around the world. In 2017, the World Health Organization published a list of 12 top-priority antibiotic-resistant pathogenic bacteria for which new effective antibiotics or new ways of treating the infections caused by them are needed. This review focuses on Acinetobacter baumannii, one of these top-priority pathogens. The pathogenic bacterium A. baumannii is one of the most frequently encountered infectious agents in the world; its clinically significant features include resistance to UV light, drying, disinfectants, and antibiotics. This review looks at the various attempts that have been made to tackle the problem of drug resistance relating to A. baumannii variants without the use of antibiotics. The potential of bacteriophages and antimicrobial peptides in the treatment of infections caused by A. baumannii in both planktonic and biofilm form is assessed. Such topics as research into the development of vaccines based on the outer membrane proteins of A. baumannii and the use of silver nanoparticles, as well as photodynamic and chelate therapy, are also covered.

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The inhibitory effect of the combination of two new peptides on biofilm formation by Acinetobacter baumannii

Cited by 18 publications

References 44 publications

An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation

An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation

Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review

Development of antimicrobial therapy methods to overcome the antibiotic resistance of Acinetobacter baumannii

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