The Inhibitory Effect of Probiotic Bacteria against Drug - Resistant Candida Species Isolated from the Oral Cavity of the Elderly

Biyari, Sepide; Fozouni, Leila

doi:10.5812/semj.62026

Cited by 8 publications

(10 citation statements)

References 12 publications

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“…P. acidilactici inhibited the Candida species growth at 8 to 512μg/mL concentrations. Our finding is in line with studies that reported the inhibitory activities of different probiotic bacteria against Candida species (Köhler, Assefa et al, 2012, Kheradmand, Rafii et al, 2014, Jiang, Stamatova et al, 2015, Shokryazdan, Jahromi et al, 2017, Biyari and Fozouni 2018. Whilst, Koll et al (2008) found no antifungal activity of lactobacillus species against Candida species (Koll, Mandar et al, 2008).…”

Section: Discussionsupporting

confidence: 92%

Morphogenesis and Pathogenesis Regulation of Candida Albicans by Probiotic Bacterium – Pediococcus Acidilactici

Rezaie

Zareshahrabadi²,

Zomorodian³

et al. 2020

J microb biotech food sci

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The transition of Candida albicans from yeast to hyphae cause the attachment to epithelial cells, forming biofilm and invasion. Therefore, the effect of Pediococcus acidilactici on morphogenesis and pathogenesis of C. albicans were examined. Inhibitory activity of P. acidilactici on Candida species growth was investigated and inhibition of C. albicans biofilm formation was measured by XTT method. Also, expression of seven genes as: Agglutinin-like protein 1 (ALS1,3), hyphal cell-wall protein (HWP1), secreted aspartyl proteinase (SAP4,6), Enhanced filamentous growth 1 (EFG1) and Enhanced activated protein 1 (EAP1) were studied by RT-PCR with different concentrations of P. acidilactici. The experimental activity of this probiotic bacterium was evaluated in an animal model by culture and histopathological methods. P. acidilactici inhibited the growth of Candida species at concentrations of 8-512 µg/mL (approximately 8 × 106 to 5 × 108 CFU/ml). This probiotic bacterium inhibited the germ tube and biofilm formation in a dose-dependent manner. RT-PCR analysis showed a reduction in genes expression. The P. acidilactici reduced the CFUs in mice receiving this probiotic bacterium. Histopathological analyses showed that Candida colonization was diminished in mice following the administration of probiotic. Since the wide range of antifungal activity of this bacterium bacterium, it can be used to manage oral candidiasis.

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Section: Discussionsupporting

confidence: 92%

Morphogenesis and Pathogenesis Regulation of Candida Albicans by Probiotic Bacterium – Pediococcus Acidilactici

Rezaie

Zareshahrabadi²,

Zomorodian³

et al. 2020

J microb biotech food sci

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“…In general, the antimicrobial activity of LAB species is well known [ 23 ]. Various investigators have demonstrated anticandidal effects of different LAB species including L. acidophilus [ 24 ], L. plantarum [ 25 ], L. paracasei [ 26 ], L. rhamnosus [ 15 ], L. reuteri [ 7 ], L. casei [ 27 ], and other clinical isolates of Lactobacillus . In this study, the antifungal effects of both cells and CFSs of L. acidophilus and L. plantarum were investigated against different oral Candida species by co-aggregation, agar overlay interference and broth microdilution methods, respectively.…”

Section: Discussionmentioning

confidence: 99%

Antifungal effects of Lactobacillus acidophilus and Lactobacillus plantarum against different oral Candida species isolated from HIV/ AIDS patients: an in vitro study

Salari

Almani

2020

Journal of Oral Microbiology

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Oropharyngeal Candidiasis (OPC) is an opportunistic fungal infection occurring in immunocompromised patients such as HIV/AIDS. The purpose of this study was to evaluate the antifungal properties of Lactobacillus acidophilus and Lactobacillus plantarum on different Candida species isolated from oral cavity of HIV/AIDS patients compared to Fluconazole (FLC). In this study, the antifungal effects of both cells and cell-free supernatants (CFSs) of L. acidophilus and L. plantarum were investigated against different oral Candida species by coaggregation, agar overlay interference and broth microdilution assays, respectively. Our results showed that the highest co-aggregation ratio of the two tested Lactic acid bacteria (LAB) was observed for C. krusei. Both L. acidophilus and L. plantarum at cell concentrations 10 10 to 10 2 cfu/ml were able to inhibit the growth of most of the oral Candida species, except for C. albicans, and to some C. krusei. In this study, MIC and MFC values for CFS of L. acidophilus ranged from 100 to 200 µl/ml and 100 to 200 µl/ml, respectively, and MIC and MFC values for CFS of L. plantarum were 50 to 200 µl/ml and 50 to 200 µl/ml, respectively. The ranges of MIC and MFC for FLC were 256-1024 µg/ml and 512-2048 µg/ ml, respectively. C. albicans and C. parapsilosis displayed the highest and least susceptibility to CFSs of two LAB, respectively. Our findings showed that both cells and CFSs of L. acidophilus and L. plantarum had antifungal effects against oral Candida species.

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“…These dilutions were used to exam the inhibitory activity of bacteriocin against indicator yeast by agar well diffusion assay (Biyari and Fozouni, 2018). Bacteriocin activity was expressed as AU/ml and defined as the reciprocal of the highest dilution showing a distinct inhibition zone of the indicator yeast.…”

Section: Bacteriocin Activity Assaymentioning

confidence: 99%

“…In order to choose the best method for concentration and partial purification of bacteriocin, three methods were used. All the methods were performed twice and the antimicrobial activity of bacteriocin in all of these methods was determined by the agar well diffusion method (Biyari and Fozouni, 2018):…”

Section: Partial Purification Of Bacteriocinmentioning

confidence: 99%

Partial Purification and Characterization of Plantaricin Wzd3, a Bacteriocin Produced by Lactobacillus Plantarum Wzd3

Mohsin¹,

Ali²

2021

PLANT ARCHIVES

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For partial purification of plantaricin WZD3, a bacteriocin produced by Lactobacillus plantarum WZD3 which has antifungal activity against Candida albicans, three procedures were performed; adsorption-desorption method, coldacetone extraction method and n-butanol extraction method. The results showed that n-butanol extraction method was the best since the activity of bacteriocin increased to 320 AU/ml. Characterization of plantaricin WZD3were also studied and the results showed that this bacteriocin have high thermostability at different temperatures, it remained active without losing its activity after being treated with (20-80) °C for (10, 30 and 60) minutes, but it retained only 50% of its activity after treatment at 100°C at the same periods of exposure and at 121°C for 15 minutes. Also, the activity of bacteriocin was stable at pH values (4-7) while at the pH values (8-11), the antifungal activity was decreased to the half, on the other hand, complete loss of activity was observed at pH (2, 3 and 12). Antifungal activity of plantaricin WZD3 was disappeared when it treated with proteolytic enzymes (pepsin and trypsin), whereas it retained whole activity when treated with lysozyme and α-amylase, indicating pure proteinaceous nature of it. The results of effect of storage period revealed that the activity of plantaricin WZD3 was gradually decreased during storage. In conclusion, plantaricin WZD3 could be a good candidate as antifungal agent and its properties are promising to use it as biopreservative instead of chemicals preservatives.

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The Inhibitory Effect of Probiotic Bacteria against Drug - Resistant Candida Species Isolated from the Oral Cavity of the Elderly

Cited by 8 publications

References 12 publications

Morphogenesis and Pathogenesis Regulation of Candida Albicans by Probiotic Bacterium – Pediococcus Acidilactici

Morphogenesis and Pathogenesis Regulation of Candida Albicans by Probiotic Bacterium – Pediococcus Acidilactici

Antifungal effects of Lactobacillus acidophilus and Lactobacillus plantarum against different oral Candida species isolated from HIV/ AIDS patients: an in vitro study

Partial Purification and Characterization of Plantaricin Wzd3, a Bacteriocin Produced by Lactobacillus Plantarum Wzd3

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