The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay

Habte, Habtom H.; Beer, Corena de; Lotz, Zoë; Tyler, Marilyn; Schoeman, L; Kahn, Delawir; Mall, Anwar Suleman

doi:10.1186/1743-422x-5-59

Cited by 23 publications

(33 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the findings in this study, and in the studies referred to above, we find it most likely that TFF3 and perhaps also other TFFs, affect the viscoelastic properties of the CMP, thereby maintaining the ability of the CMP to protect against ascending infections from the vagina to the uterine cavity (5,9,26), yet the exact nature of TFF's interaction with mucins is still to be fully explained.…”

Section: Discussionsupporting

confidence: 55%

“…Initially, CMPs were collected from 16 women in active labor (cervical dilatation 4-10 cm) at the delivery wards of Aarhus University Hospital, Denmark. The median (range) age of the women included was 29.5 (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36) years, the median gestational age was 40 +1 weeks (38 +0 -42 +0 weeks) and median parity was 1.5 (1)(2)(3)(4). The CMPs were shed spontaneously or manually retrieved during vaginal exploration.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Trefoil factor peptide 3 is positively correlated with the viscoelastic properties of the cervical mucus plug

Bastholm

Samson

Becher

et al. 2016

Acta Obstet Gynecol Scand

View full text Add to dashboard Cite

Introduction The viscoelastic properties of the cervical mucus plug are considered essential for the occlusion of the cervical canal and thereby for protection against ascending infections during pregnancy. Factors controlling this property are virtually unknown. This study explores a possible role of trefoil factor peptides 1, 2 and 3 (TFF1–3); peptides believed to influence mucus viscosity. Material and methods The study is based on spontaneously shed cervical mucus plugs from 14 women in active labor. The viscoelastic properties; the elastic modulus (G') and the viscous modulus (G”) were determined by an oscillatory rheometer. The concentrations of TFF1–3 were measured by an in‐house enzyme‐linked immunosorbent assay. Associations were analyzed by random‐effects generalized least‐squares regression analyses. Results Median (range) concentrations of TFF1, TFF2 and TFF3 were 3.1 (1.2–8.6), 1.1 (<0.006–3.7) and 1000 (170–5300) nmol/g cervical mucus plug, respectively. The TFF3 concentration was associated with G' (regression coefficient 11.7 Pa/Log nm; 95% CI 3.0–20.4, p = 0.009) and G” (regression coefficient 3.2 Pa/Log nm; 95% CI 1.5–5.0, p < 0.001). Conclusion We suggest that TFF3 plays a role in the viscoelastic properties of the cervical mucus plug.

show abstract

Section: Discussionsupporting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Trefoil factor peptide 3 is positively correlated with the viscoelastic properties of the cervical mucus plug

Bastholm

Samson

Becher

et al. 2016

Acta Obstet Gynecol Scand

View full text Add to dashboard Cite

show abstract

“…We hypothesized and confirmed that salivary MUC5B and MUC7 [4], breast milk mucin (MUC1) [5] and cervical or pregnancy plug mucins [6] inhibited HIV-1 activity in an in vitro inhibition assay. We have also shown the inhibition of poxvirus activity by MUC1 [7].…”

Section: Introductionmentioning

confidence: 85%

Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts

Habte

Beer

Lotz

et al. 2010

Virol J

Self Cite

View full text Add to dashboard Cite

BackgroundWe have previously shown that MUC5B and MUC7 mucins from saliva of HIV negative individuals inhibit HIV-1 activity by 100% in an in vitro assay. The purpose of this subsequent study was to investigate whether MUC5B and MUC7 from saliva of HIV patients or with full blown AIDS had a similar inhibitory activity against the virus.MethodsSalivary MUC5B and MUC7 from HIV patients with different CD4 counts (< 200, 200-400 and > 400) were incubated with HIV-1 prior to infection of the human T lymphoblastoid cell line (CEM SS cells). Cells were then cultured and viral replication was measured by a qualitative p24 antigen assay. The size, charge and immunoreactivity of mucins from HIV negative and positive individuals was also analysed by SDS-PAGE, Western blot and ELISA respectively.ResultsIt was shown that irrespective of their CD4 counts both MUC5B and MUC7 from HIV patients, unlike the MUC5B and MUC7 from HIV negative individuals, did not inhibit HIV-1 activity. Size, charge and immunoreactivity differences between the mucins from HIV negative and positive individuals and among the mucins from HIV patients of different CD4 count was observed by SDS-PAGE, Western blot and ELISA.ConclusionsPurified salivary mucins from HIV positive patients do not inhibit the AIDS virus in an in vitro assay. Although the reason for the inability of mucins from infected individuals to inhibit the virus is not known, it is likely that there is an alteration of the glycosylation pattern, and therefore of charge of mucin, in HIV positive patients. The ability to inhibit the virus by aggregation by sugar chains is thus diminished.

show abstract

“…Mucins provide the structural framework of the CMP, and the extraction of mucins for analysis is a challenging procedure. Therefore, guanidinium chloride has been generally used to extract mucins [7,54–56]. As a reducing agent was not used in our protein isolation protocol, the mucins remain cross-linked to each other to a substantial degree and so are not represented to the extent that they are present in the CMP.…”

Section: Discussionmentioning

confidence: 99%

“…The CMP is a unique and sizable (approximately 10 gm) structure that forms physical and biochemical barriers for the microorganisms present in the vagina [6]. It has been demonstrated that mucin proteins in the CMP, such as MUC1, MUC2, MUC5AC, and MUC5B, profoundly inhibit the activity of human immunodeficiency virus type I [7]. The biological and physical integrity of the CMP is therefore critical for a successful pregnancy because its disruption can lead to ascending intra-amniotic infection, one of the leading causes of preterm birth [6,8].…”

Section: Introductionmentioning

confidence: 99%

Protein profiling underscores immunological functions of uterine cervical mucus plug in human pregnancy

Lee

Hassan

Romero

et al. 2011

Journal of Proteomics

View full text Add to dashboard Cite

The cervical mucus plug (CMP) differs from the cervical secretions of non-pregnant women, and is the ultimate sealant of the uterine cavity during pregnancy. Although several studies have analyzed biochemical properties of large glycoproteins in the CMP, comprehensive information about its protein composition is yet unavailable. We hypothesized that protein profiling of the CMP could provide key clues to its physiological functions in pregnancy. For this purpose, five CMPs obtained from women in labor at term were analyzed by LC-MS/MS. Out of 291 total proteins identified, 137 were detected in two or more samples, which included S100A8, S100A9, and complement proteins (C3, C4a, C4b, C6, C8g). Several proteins, which have not been described in the cervical mucus of non-pregnant women or in cervicovaginal fluids, such as CD81 antigen and pregnancy zone protein, were also identified. Gene ontology analysis of identified proteins showed significant enrichment of 28 biological processes such as ‘activation of plasma proteins involved in acute inflammatory response’ and ‘positive regulation of cholesterol esterification’. We report the proteome of CMPs from pregnant women at term for the first time, and the overall findings strongly suggest an important role for the CMP in the maintenance of pregnancy and parturition.

show abstract

The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay

Cited by 23 publications

References 30 publications

Trefoil factor peptide 3 is positively correlated with the viscoelastic properties of the cervical mucus plug

Trefoil factor peptide 3 is positively correlated with the viscoelastic properties of the cervical mucus plug

Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts

Protein profiling underscores immunological functions of uterine cervical mucus plug in human pregnancy

Contact Info

Product

Resources

About