The influence of the chemical composition of cell culture material on the growth and antibody production of hybridoma cells

Heilmann, Katja; Groth, Thomas; Behrsing, Olaf; Albrecht, Wolfgang; Schossig, Michael; Lendlein, Andreas; Micheel, Burkhard

doi:10.1016/j.jbiotec.2004.09.004

Cited by 17 publications

(12 citation statements)

References 18 publications

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“…Furthermore, we found that, though fewer ECs adhered, the MTT viability of ECs adhering to PANC-NVP22 and PANCNVP31 was relatively high, which was in accordance with previous reports [12,15,42,45]. It is generally accepted that the physicochemical properties of interfacial water profoundly influence the biological response to materials [46].…”

Section: Discussionsupporting

confidence: 92%

“…They found these films to be highly hydrophilic and suitable for seeding ECs. Groth et al [15,42,45] reported the culture of human skin fibroblasts, hepatocyte and hybridoma cells on PANCNVP membrane surfaces. It was found that the cells attached and spread better on the PAN membrane surface than on the PANCNVP ones, while the functions of the attached cells were just the reverse, e.g.…”

Section: Discussionmentioning

confidence: 99%

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Cytocompatibility of poly(acrylonitrile-co-N-vinyl-2-pyrrolidone) membranes with human endothelial cells and macrophages

Wan

Huang

et al. 2007

Acta Biomaterialia

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Cytocompatibility of poly(acrylonitrile-co-N-vinyl-2-pyrrolidone) membranes with human endothelial cells and macrophages

Wan

Huang

et al. 2007

Acta Biomaterialia

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“…␤ 2 -microglobulin) [9]. Furthermore, such membranes can be utilized as suitable materials for development of bioreactors since they could provide favourable conditions for monoclonal antibody production of murine hybridoma cells [7]. As PAN is a hydrophobic material, different copolymerization strategies have been applied to improve cell-or hemocompatibility of PAN-based membranes by copolymerization with hydrophilic comonomers (e.g.…”

Section: Introductionmentioning

confidence: 99%

Melt-processable hydrophobic acrylonitrile-based copolymer systems with adjustable elastic properties designed for biomedical applications

Cui

Trescher

Kratz

et al. 2010

Clinical Hemorheology and Microcirculation

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Acrylonitrile-based polymer systems (PAN) are comprehensively explored as versatile biomaterials having various potential biomedical applications, such as membranes for extra corporal devices or matrixes for guided skin reconstruction. The surface properties (e.g. hydrophilicity or charges) of such materials can be tailored over a wide range by variation of molecular parameters such as different co-monomers or their sequence structure. Some of these materials show interesting biofunctionalities such as capability for selective cell cultivation. So far, the majority of AN-based copolymers, which were investigated in physiological environments, were processed from the solution (e.g. membranes), as these materials are thermo-sensitive and might degrade when heated.In this work we aimed at the synthesis of hydrophobic, melt-processable AN-based copolymers with adjustable elastic properties for preparation of model scaffolds with controlled pore geometry and size. For this purpose a series of copolymers from acrylonitrile and n-butyl acrylate (nBA) was synthesized via free radical copolymerisation technique. The content of nBA in the copolymer varied from 45 wt% to 70 wt%, which was confirmed by 1 H-NMR spectroscopy. The glass transition temperatures (T g ) of the P(AN-co-nBA) copolymers determined by differential scanning calorimetry (DSC) decreased from 58 • C to 20 • C with increasing nBA-content, which was in excellent agreement with the prediction of the Gordon-Taylor equation based on the T g s of the homopolymers. The Young's modulus obtained in tensile tests was found to decrease significantly with rising nBAcontent from 1062 MPa to 1.2 MPa. All copolymers could be successfully processed from the melt with processing temperatures ranging from 50 • C to 170 • C, whereby thermally induced decomposition was only observed at temperatures higher than 320 • C in thermal gravimetric analysis (TGA). Finally, the melt processed P(AN-co-nBA) biomaterials were sterilized with ethylene oxide and tested for cytotoxicity in direct contact tests with L929 cells according to the EN DIN ISO standard 10993-5. All tested samples exhibited non-toxic effects on the functional integrity of the cell membrane and the mitochondrial activity. However, the morphology of the cells on the samples was different from that observed on polystyrene as control, indicating slightly cytotoxic effects according to the evaluation guide of the US Pharmacopeial Convention. Thus, the melt-processable, hydrophobic P(ANco-nBA) copolymers with adjustable mechanical properties are promising candidates for in vitro investigations of tissue growth kinetics.

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“…The commonly used culture systems for the production of mAb by hybridoma cells are the Stirred Tank (ST) bioreactor and airlift fermenter (Ozturk and Palsson 1991;Guez et al 2004) and in some occasions the hollow-fibre and ceramic matrix module has been used (Butler 1996;Jackson et al 1996;Racher et al 1990;Heilmann et al 2005). Among those bioreactor systems, the ST bioreactor which is the traditionally used fermenter with the internal mechanical agitation is preferable in improving the mAb production because it is highly flexible and it can provide high volumetric mass-transfer coefficient (k L a) values for gas transfer.…”

Section: Introductionmentioning

confidence: 99%

Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor

Mel

Rahman

Salleh

et al. 2008

World J Microbiol Biotechnol

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The study was done to improve the viability of the RC1 hybridoma cell in order to produce more amount of monoclonal antibody (mAb). By using the optimized media, the cell had been cultured in two bioreactor systems which were the MiniPerm and Stirred Tank bioreactor (ST bioreactor), and the results were compared to the one obtained by using the T-Flask bioreactor which was used as a standard. The results showed that the ST bioreactor was able to improve the viability of the cell to the value of 91.8% which was a little bit better than the one obtained by the MiniPerm bioreactor (88.6%) and far better than that of achieved by the T-Flask bioreactor (76.4%). This was well correlated with the good growth performance of the cell in the ST bioreactor with the specific growth rate (l) value of 0.0289 h -1 followed by MiniPerm bioreactor with the value of 0.0243 h -1 and then the T-Flask with the value of 0.0151 h -1 . The low value of doubling time (t d ) obtained in the ST bioreactor (24 h) compared to the one obtained in the MiniPerm (29 h) and T-Flask bioreactor (46 h) had also contributed to the higher value of cell viability. As a result a higher concentration of mAb was able to be produced by the ST bioreactor (0.42 g l -1 ) compared to that of the MiniPerm (0.37 g l -1 ) and T-Flask bioreactor (0.23 g l -1 ).

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The influence of the chemical composition of cell culture material on the growth and antibody production of hybridoma cells

Cited by 17 publications

References 18 publications

Cytocompatibility of poly(acrylonitrile-co-N-vinyl-2-pyrrolidone) membranes with human endothelial cells and macrophages

Cytocompatibility of poly(acrylonitrile-co-N-vinyl-2-pyrrolidone) membranes with human endothelial cells and macrophages

Melt-processable hydrophobic acrylonitrile-based copolymer systems with adjustable elastic properties designed for biomedical applications

Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor

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