The influence of protein adsorption on interactions of cultured human endothelial cells with polymers

Wachem, P.B. van; Vreriks, C. M.; Beugeling, T.; Feijén, Jan; Bantjes, A.; Detmers, J. P.; Aken, W.G. van

doi:10.1002/jbm.820210603

Cited by 155 publications

(100 citation statements)

References 27 publications

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“…Fibronectin adsorption displays a maximum in adsorption at intermediate serum dilutions, a phenomenon similar to the maximum in fibrinogen adsorption at intermediate plasma dilutions (19,67). A corresponding maximum in cell spreading or attachment has been observed in several studies, using BHK cells (68), platelets (69), 3T3 cells (67), and endothelial cells (70). The magnitude and position of the peak in adsorption of fibronectin depends somewhat on the surface composition of the substrate (60).…”

Section: The Role Of Adsorbed Proteins In Cell Interactions With Solimentioning

confidence: 96%

Proteins at Interfaces

Brash

Horbett

1995

ACS Symposium Series

203

119

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Proteins at interfaces are involved in a wide variety of phenomena, including mammalian cell growth in culture, reactions to implanted biomaterials, growth of soil bacteria, and formation of organized layers of proteins at the oil/water and air/water interfaces.This broad range of phenomena has attracted the interest of a correspondingly broad range of scientists and engineers, many with different backgrounds and different perspectives. As a result, the field of proteins at interfaces displays as much breadth as depth, and many of the investigators in this field of research conduct investigations that are unique with respect both to the particular protein/surface system studied and to the methods used. The many different approaches and systems under investigation, and in particular the wide variety of applications towards which the research is directed, means that an overview such as this one which is limited in length, must be selective. In selecting subtopics for discussion we had to be somewhat arbitrary, and therefore tried to focus on broad aspects which are common to most application areas.The contributions to the San Diego Symposium and to this volume represent a significant fraction of the workers who are actively involved in studying the behavior of proteins at interfaces. We have therefore chosen to organize our overview around six major aspects of protein behavior at interfaces which emerged from the Symposium. These aspects are: (1) theory, including molecular mechanisms; (2) competitive adsorption; (3) conformation and orientation of proteins at interfaces; (4) surface chemistry effects on adsorption; (5) the behavior of proteins at fluid interfaces; and (6) effects of proteins on cell interactions with surfaces.For each topic, current understanding is briefly summarized, and the contributions of the articles in this volume are then discussed. Inevitably this type of subdivision is to some extent arbitrary and artificial, so there is some overlap of material from one section to another. We trust, however, that on balance our approach will facilitate the readers' (as well as the authors') task of understanding the state of the art in this field.0097-6156/95/0602-0001$12.75/0

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Section: The Role Of Adsorbed Proteins In Cell Interactions With Solimentioning

confidence: 96%

Proteins at Interfaces

Brash

Horbett

1995

ACS Symposium Series

203

119

View full text Add to dashboard Cite

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“…As a consequence, a decrease in the amount of a preadsorbed protein will not be detected if this amount is not very much reduced during exposure to the cell suspension [11]. By decreasing the serum concentration in the solutions used for pretreatment of the polymer surfaces, the amounts of adsorbed proteins are generally smaller [10]. Consequently, the endothelial cells had to be suspended in medium lacking serum to avoid adsorption of large amounts of serum proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, protein adsorption is one of the major determinants for adhesion and spreading of cells on polymers. Research on protein adsorption in our laboratory revealed that the amount of adsorbed fibronectin, a protein which promotes cell adhesion, from culture medium containing 20% human serum to TCPS is relatively small [10]. However, from this culture medium, relatively large amounts of highdensity lipoprotein, immunoglobulin G and human serum albumin (HSA), which all inhibit cell adhesion, adsorb to TCPS.…”

confidence: 99%

“…However, from this culture medium, relatively large amounts of highdensity lipoprotein, immunoglobulin G and human serum albumin (HSA), which all inhibit cell adhesion, adsorb to TCPS. In spite of the high adsorption of these proteins, endothelial cells adhere and spread well on TCPS [3,10]. Moreover, endothelial celt-derived fibronectin is deposited in relatively large amounts on to TCPS during adhesion and spreading [11,12].…”

confidence: 99%

“…The two-step enzyme-immunoassay was carried out as described by Van Wachem et al [10]. This assay was performed in the following way.…”

Section: Determination Of the Amounts Of Adsorbed Proteinsmentioning

confidence: 99%

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Deposition of cellular fibronectin and desorption of human serum albumin during adhesion and spreading of human endothelial cells on polymers

Dekker

Beugeling

Wind

et al. 1991

J Mater Sci: Mater Med

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More insight into the mechanism of adhesion of human endothelial cells (HEC) on to polymeric surfaces may lead to the development of improved small-diameter vascular grafts. H EC suspended in 20% human serum-containing culture medium adhere and spread well on moderately water-wettable polymers such as tissue culture polystyrene (TCPS). Earlier it was demonstrated that during adhesion and spreading of HEC on TCPS, cellular fibronectin is deposited on to this surface. It was postulated that fibronectin deposition is accompanied by desorption of adsorbed serum proteins, e.g. human serum albumin (HSA). The amounts of adsorbed (cellular) fibronectin and HSA on TCPS surfaces pretreated for 1 h with solutions of human serum (ranging from 0.01%-20%), were determined after incubation of these surfaces for 6 h with HEC in culture medium and after incubation with culture medium without cells. Protein adsorption was determined by means of a two-step enzyme-immunoassay (EIA). HEC adhesion and spreading on TCPS resulted in a significant deposition of fibronectin irrespective of the serum concentration in the solution used for the pretreatment of TCPS. The deposition of cellular fibronectin on to TCPS, pretreated with human serum, was accompanied by displacement of adsorbed HSA. Desorption of HSA from TCPS was only detectable with the EIA at serum concentrations ranging from 0.01%-1%. Using l~l-I-labelled HSA as tracer protein; it could, however, be demonstrated that HSA was also displaced from TCPS, pretreated with solutions of higher serum concentrations. Pretreatment of the hydrophobic vascular graft material PET (poly(ethylene terephthalate); Dacron) and of FEP (fluoroethylenepropylene copotymer; a Teflon-like polymer) with a solution containing 20% human serum resulted in a reduced adhesion of HEC compared to uncoated surfaces. We suggest that this may be caused by a poor displacement of adsorbed serum proteins from these hydrophobic surfaces by cellular fibronectin. This may explain why HEC normally fail to adhere on to prosthetic surfaces.

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Physicochemical and biological studies of corona-treated artificial membranes used for pancreatic islets encapsulation: Mechanism of diffusion and interface modification

Kessler¹,

Legeay²,

West³

et al. 1997

J. Biomed. Mater. Res.

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The artificial AN69 membrane (Hospal), a synthetic copolymer composed of acrylonitrile and sodium methallyl sulphonate suitable for pancreatic islet encapsulation, was submitted to physicochemical treatment (Corona discharge) to improve its insulin permeability. X-ray photoelectron spectroscopy (XPS) analysis of the AN69 membrane indicated the presence of up to two molecular layers of glycerol at its surface while the surface energies revealed the presence of hydrophilic sites (-SO3Na/glycerol) located at the membrane surface and acrylonitrile hydrophobic groups inside the material. The Corona discharges decreased the number of glycerol molecules at the membrane surface and from a biological point of view, produced a threefold increase in insulin diffusion. Furthermore, the biocompatibility of the treated membrane was preserved after 1 year of intraperitoneal implantation. The increase in insulin permeability should result from a decrease of the membrane polarity and of a steric hindrance in pores. Thus, Corona discharge treatment may serve to optimize the properties of artificial membranes used for pancreatic islets encapsulation.

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The influence of protein adsorption on interactions of cultured human endothelial cells with polymers

Cited by 155 publications

References 27 publications

Proteins at Interfaces

Proteins at Interfaces

Deposition of cellular fibronectin and desorption of human serum albumin during adhesion and spreading of human endothelial cells on polymers

Physicochemical and biological studies of corona-treated artificial membranes used for pancreatic islets encapsulation: Mechanism of diffusion and interface modification

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