The Influence of Oxygen Tension on Cumulus Cell Viability of Canine COCs Matured in High‐Glucose Medium

Silva, A E F; Rodriguez, P.; Cavalcante, Liziane Ferraresi Holanda; Rodrigues, B. A.; Rodrigues, José Ariévilo Gurgel

doi:10.1111/j.1439-0531.2009.01406.x

Cited by 17 publications

(15 citation statements)

References 27 publications

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“…5 vs 20%) (Rodrigues & Rodrigues 2010). Despite the presence of anti-apoptotic intracellular mechanisms acting via GSH against ROS (Silva et al 2009), having high lipid content in canine COCs can reduce GSH level and predispose them to oxidative damage and induce apoptosis in the cumulus cells (Silva et al 2009). This can contribute to lowered oocyte maturation rates (Kim et al 2007, Rodrigues & Rodrigues 2010.…”

Section: Oxygen Level and Ivmmentioning

confidence: 99%

“…Cumulus expansion (mucification) was described by the partial or complete loosening of cumulus cells into extracellular matrix surrounding the oocyte (Lee et al 2007a, Marei et al 2009, Silva et al 2009). COCs were assessed for expansion of cumulus cells at 24 h after the beginning of the culture and by the end of 52 h under a stereomicroscope.…”

Section: Assessment Of Cumulus Cell Expansionmentioning

confidence: 99%

“…High oxygen level has detrimental effects on cumulus cell survival (Silva et al 2009), which influences expansion during oocyte maturation in vitro. In the majority of IVM techniques in domestic animals, cumulus cell expansion and nuclear maturation are concomitant phenomena during the culture period.…”

Section: Introductionmentioning

confidence: 99%

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Effects of oxygen concentration on in vitro maturation of canine oocytes in a chemically defined serum-free medium

Salavati¹,

Ghafari²,

Zhang³

et al. 2012

Reproduction

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Canine oocytes require an extended period of culture (72 h) in vitro for nuclear maturation to the metaphase II stage, which also results in high degeneration. Canine cumulus oocyte complexes were isolated by slicing from ovaries collected after ovariohysterectomy and cultured in serum-free synthetic oviductal fluid incubated at low (5%) or high (20%) oxygen levels. Changes in oocyte nuclear maturation rates, H 2 O 2 levels within the oocytes and mRNAs of reactive oxygen species inhibitory genes superoxide dismutase 1 and 2 (SOD1 and 2), glutathione reductase (GSR), glutathione peroxidase (GPX1), and catalase (CAT) were quantified. Higher meiotic resumption from germinal vesicle breakdown up to MII was observed in low O 2 (41.8G13.1%) compared to high O 2 (15.8G8.2%) (PZ0.014) after 52 h of culture (nZ112). Extension of the culture period up to 84 h at low O 2 (nZ457 oocytes) produced the highest meiotic resumption at 72 h (64.1G6.0%; PZ0.008), compared with 52 h. Oocytes (nZ110) cultured in high O 2 contained higher levels of peroxidase measured using the 2 0 ,7 0 -dichlorodihydrofluorescein diacetate fluorescence assay after 72 h of culture compared with low O 2 (PZ0.004). High O 2 -cultured oocytes also showed higher amounts of SOD1, SOD2, GSR, GPX1, and CAT mRNA. Vitamin E in high oxygen level was able to decrease degeneration (PZ0.008) but had no improving effect on percentage of oocytes in MII. These results for the first time showed that low oxygen gas composition improves nuclear maturation rates and alleviates the oxidative stress for canine oocytes during in vitro maturation.

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Section: Oxygen Level and Ivmmentioning

confidence: 99%

Section: Assessment Of Cumulus Cell Expansionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of oxygen concentration on in vitro maturation of canine oocytes in a chemically defined serum-free medium

Salavati¹,

Ghafari²,

Zhang³

et al. 2012

Reproduction

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“…Despite the fact that there are several studies with O 2 in various species of mammals, revealing that the concentration of O 2 inside the uterus and oviducts usually fluctuates in the range of 2-8% [23], we used 5% of O 2 . Silva et al [15], showed in canine CCs that low O 2 tension was efficient in reducing apoptosis, working with 5% O 2 tension level and 5% CO 2 with a media supplemented with FCS, FSH, E 2 and HCG and high levels of glucose (5.5 mM), obtaining 38.9% of apoptosis. We obtained degenerated oocyte values of 65.5±7.3, 39.6±7.1 and 28.19±6.4 for media 1, 2 and 3 respectively with 5% CO 2 during maturation, and 71.42±12.23, 27.71±10.0 and 25.33±10.2 in case of culture with the presence of O 2 and N 2 .…”

Section: Results and Discusionmentioning

confidence: 99%

“…5% CO 2 is the most common concentration in IVM oocytes studies. In relation with oxygen, high in vitro O 2 tensions are associated with enhanced levels of reactive oxygen species and cumulus oocyte complex (COC) apoptosis, so low O 2 tension was efficient in reducing apoptosis in canine oocytes [15]. However there are no studies about the influence of nitrogen on canine oocyte maturation.…”

Section: Introductionmentioning

confidence: 99%

Effect of Gaseous Atmosphere on Different Supplementation Medium for In Vitro Canine Oocyte Maturation

González¹,

Gil²,

Rios³

et al. 2014

TOVSJ

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Abstract:The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO 2 , B: 5%CO 2 , 5%O 2 and 90%N 2 ) on three different maturation media on in vitro maturation (IVM) rates for oocytes from anoestrous and diestrous bitches that were cultured in vitro for 48h. Oocytes harvested from bitches were selected and allocated into three maturation media groups, cultured in atmosphere A or B. Group 1, oestrous bitch serum; Group 2, bovine serum albumin (BSA), glutamine, cisteamine; Group 3, BSA, estrogen (E 2 ), epidermal growth factor (EGF). Progression of nuclear maturation was evaluated under fluorescent microscopy after staining with Hoechst 33342 solution.The results demonstrated that different maturation media was not influenced by the atmosphere in any case. Group 1 showed the lowest maturation rates in both cases: 4.3±1.3 vs 7.3±2.1 and 7.9±2.5 for groups 1, 2 and 3 respectively in atmosphere A (p≤ 0.5), 0 vs 7.2± 2.9 and 9.3± 4.0 in atmosphere B (p= 0.33). Maturation rates were similar among treatments 2 and 3 in both atmospheres, were slightly higher for treatment 3. Moreover, group 1 obtained the highest degeneration rates: 65.5±7.3 vs 39.63±7.1, 28.19±6.4 (p= 0.018) and 71.43±12.2 vs 27.71±10, 25.33± 10.20 (p= 0.010) for mediums 1, 2, and 3 in atmospheres A and B respectively.Our findings indicate that atmosphere B seems to be more favorable habitat for canine oocytes and independently of gassed, maturation with not controlled supplementation, with estrous bitch serum, is not a good option.

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Primate Follicular Development and Oocyte Maturation In Vitro

Bernuci

et al. 2013

Advances in Experimental Medicine and Biology

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The factors and processes involved in primate follicular development are complex and not fully understood. An encapsulated three-dimensional (3D) follicle culture system could be a valuable in vitro model to study the dynamics and regulation of folliculogenesis in intact individual follicles in primates. Besides the research relevance, in vitro follicle maturation (IFM) is emerging as a promising approach to offer options for fertility preservation in female patients with cancer. This review summarizes the current published data on in vitro follicular development from the preantral to small antral stage in nonhuman primates, including follicle survival and growth, endocrine (ovarian steroid hormone) and paracrine/autocrine (local factor) function, as well as oocyte maturation and fertilization. Future directions include major challenges and strategies to further improve follicular growth and differentiation with oocytes competent for in vitro fertilization and subsequent embryonic development, as well as opportunities to investigate primate folliculogenesis by utilizing this 3D culture system. The information may be valuable in identifying optimal conditions for human follicle culture, with the ultimate goal of translating the experimental results and products to patients, thereby facilitating diagnostic and therapeutic approaches for female fertility.

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The Influence of Oxygen Tension on Cumulus Cell Viability of Canine COCs Matured in High‐Glucose Medium

Cited by 17 publications

References 27 publications

Effects of oxygen concentration on in vitro maturation of canine oocytes in a chemically defined serum-free medium

Effects of oxygen concentration on in vitro maturation of canine oocytes in a chemically defined serum-free medium

Effect of Gaseous Atmosphere on Different Supplementation Medium for In Vitro Canine Oocyte Maturation

Primate Follicular Development and Oocyte Maturation In Vitro

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