The influence of N-acetyl-l-cysteine on damage of porcine oocyte exposed to zearalenone in vitro

Lai, Fang-Nong; Ma, Jun‐Yu; Liu, Jing-Cai; Wang, Junjie; Cheng, Shun‐Feng; Sun, Xiao‐Feng; Li, Lan; Li, Bo; Nyachoti, C. M.; Shen, Wei

doi:10.1016/j.taap.2015.09.010

Cited by 54 publications

(33 citation statements)

References 38 publications

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“…Unsuccessful migration of mitochondria to the inner cytoplasm is involved in failed cytoplasmic maturation . During the maturation of mouse oocytes, mitochondria were translocated homogeneously from the perinuclear area to the cytoplasm . Our results showed that in FE‐treated oocytes, homogeneously located mitochondria were significantly reduced (Fig.…”

Section: Discussionmentioning

confidence: 57%

Fenoxaprop‐ethyl affects mouse oocyte quality and the underlying mechanisms

Yang

Zhao

et al. 2018

Pest Management Science

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Section: Discussionmentioning

confidence: 57%

Fenoxaprop‐ethyl affects mouse oocyte quality and the underlying mechanisms

Yang

Zhao

et al. 2018

Pest Management Science

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“…In nature, animals cannot avoid eating or touching feed or raw materials containing ZEA . ZEA is structurally similar to estrogen, and can compete with estrogen to bind estrogen receptor, thus interfering with the effect of estrogen . ZEA also has a similar structure to steroids.…”

Section: Introductionmentioning

confidence: 99%

Zearalenone induces apoptosis of rat Sertoli cells through Fas‐Fas ligand and mitochondrial pathway

Cai

et al. 2019

Environmental Toxicology

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Zearalenone (ZEA) is an estrogen-like toxin produced by Fusarium that is widely found in cereals worldwide. In recent years, ZEA has been found to cause reproductive dysfunction in male animals, but the underlying mechanism remains unclear. This study examined the apoptosis of rat Sertoli cells induced by different concentrations (0, 5, 10, and 20 μmol/L) of ZEA via Fas-Fas ligand and mitochondrial signaling pathway in vitro. Apoptosis rate was detected by flow cytometry. The mitochondrial membrane potential was detected by immunofluorescence assay and flow cytometry. Western Blot and qRT-PCR were used to identify the signaling pathway. The results revealed that ZEA induced apoptosis of rat Sertoli cells, significantly reduced the transcription and expression of the anti-apoptotic protein Bcl-2, increased the transcription and expression of pro-apoptotic proteins Bax and tBID, and Fas, FasL, FADD, and caspase-8. ZEA also increased the activation of caspase-8 and caspase-9, and promoted the release of cytochrome C from mitochondria to cytoplasm. Moreover, addition of caspase-8 inhibitor Z-IETD-FMK led to significant decrease in the mitochondrial membrane potential and apoptosis rate of the ZEA + Z-IETD-FMK group as compared to the ZEA treatment group. The release of cytochrome C from mitochondria to cytoplasm and the activation of caspase-9 and caspase-3 were significantly decreased in the ZEA + Z-IETD-FMK group. These results suggested that ZEA can induce apoptosis of rat Sertoli cells, activate the Fas-Fas ligand signaling pathway and participate in the regulation of mitochondrial apoptosis pathway. K E Y W O R D S apoptosis, Fas ligand, mitochondria, Sertoli cells, Zearalenone | INTRODUCTIONZearalenone (ZEA), also known as F-2 toxin, is a mycotoxin produced by fusarium spp, which is commonly found in feed and food. In nature, animals cannot avoid eating or touching feed or raw materials containing ZEA. 1 ZEA is structurally similar to estrogen, and can compete with estrogen to bind estrogen receptor, thus interfering with the effect of estrogen. 2 ZEA also has a similar structure to steroids. By competitive binding of 3α-HSD and 3β-HSD, the synthesis of reproductive hormones is affected, which leads to disorders of reproductive system function. 3 The estrogen receptor is the main target of zearalenone and its metabolites. 4 Early studies found that the degradation rate of porcine oocytes increased with the increase of α-ZEA and β-ZEA doses. 5 A study in which cows were fed with ZEA containing feeds showed that the pregnant cows had oestrus, while the pubertal cows were estrous but sterile. 6 ZEA can also affect the reproduction of male animals. High dose of ZEA and its metabolites could reduce the survival rate of sperms, increase the rate of deformity and mortality, decrease the concentration of sperms, and damage the reproductive function of male animals. 7,8 Cheraghi et al. showed that the interaction of cyclin D1 and E2F1 during mitosis resulted in an increase in apoptosis after subcutaneous injection of 1-...

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“…There are a number of examples to show that ZEA increases the oxidative stress in farm animals 9 , 13 , 18 , 19 . Our previous studies have shown that ZEA increased the levels of reactive oxygen species (ROS) and repressed the activity and expression of anti-oxidative enzymes in porcine granulosa cells or oocytes at the concentrations from 15 μM to 60 μM 4 , 5 . Moreover, in post weaned gilts serum alanine aminotransferase, aspartate aminotransferase, γ-glutamate transferase and alkaline phosphatase concentrations in liver and serum were increased linearly in a ZEA dose dependent manner but activities of glutathione peroxidase and superoxide dismutase and spleen weight were decreased as dietary ZEA increased 20 .…”

Section: Introductionmentioning

confidence: 99%

Mycotoxin zearalenone exposure impairs genomic stability of swine follicular granulosa cells in vitro

Liu¹,

Wu²,

Sun³

et al. 2018

Int. J. Biol. Sci.

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Zearalenone (ZEA), a metabolite of Fusarium fungi, is commonly found on moldy grains. Because it can competitively combine to estrogen receptor to disrupt estrogenic signaling, it has been reported to have serious adverse effects on animal reproduction systems. In order to explore the genotoxic effects of ZEA exposure on ovarian somatic cells, porcine granulosa cells were exposed to 10 μM and 30 μM ZEA for 24 or 72 h in vitro. The results showed that ZEA exposure for 24 h remarkably reduced the proliferation of porcine granulosa cells in a dose-dependent manner as determined by MTT analysis and flow cytometry. Furthermore, exposure to ZEA for 72 h induced apoptosis, and RNA sequence analysis also revealed that the expression of apoptosis related genes were altered. RT-qPCR, immunofluorescence and western blot analysis further confirmed the expression of DNA damage and repair related genes (γ-H2AX, BRCA1, RAD51 and PRKDC) were increased in ZEA exposed granulosa cells. When the estrogen antagonist, tamoxifen, was added with ZEA in the culture medium, the DNA damage and repairment by ZEA returned to normal level. Collectively, these results illustrate that ZEA disrupts genome stability and inhibits growth of porcine granulosa cells via the estrogen receptors which may promote granulosa cell apoptosis when the DNA repair system is not enough to rescue this serious damage.

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The influence of N-acetyl-l-cysteine on damage of porcine oocyte exposed to zearalenone in vitro

Cited by 54 publications

References 38 publications

Fenoxaprop‐ethyl affects mouse oocyte quality and the underlying mechanisms

Fenoxaprop‐ethyl affects mouse oocyte quality and the underlying mechanisms

Zearalenone induces apoptosis of rat Sertoli cells through Fas‐Fas ligand and mitochondrial pathway

Mycotoxin zearalenone exposure impairs genomic stability of swine follicular granulosa cells in vitro

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