The influence of matrix integrity on stress-fiber remodeling in 3D

Foolen, Jasper; Deshpande, V.S.; Kanters, Fmw Frans; Baaijens, Fpt Frank

doi:10.1016/j.biomaterials.2012.06.103

Cited by 79 publications

(115 citation statements)

References 54 publications

Supporting

Mentioning

107

Contrasting

Order By: Relevance

“…[10][11][12] Thus, there is a pressing need for tools to define the mechanical responses of cells in 3D cultures, and a number of coupled experimental and analytical systems have been developed for this purpose. [13][14][15] These mechanobiological tools include ring-like tissue constructs, 16 tissue constructs adhered to flexible substrata 17 and 3D traction microscopy. 14 However, challenges persist for achieving displacement control, obtaining statistically significant sample sizes, avoiding diffusion barriers for soluble factors and avoiding perturbations introduced by sample handling before mechanical loading.…”

Section: Introductionmentioning

confidence: 99%

Magnetically actuated cell-laden microscale hydrogels for probing strain-induced cell responses in three dimensions

Huang

Gao

et al. 2016

NPG Asia Mater

View full text Add to dashboard Cite

Living cells respond to their mechanical microenvironments during development, healing, tissue remodeling and homeostasis attainment. However, this mechanosensitivity has not yet been established definitively for cells in three-dimensional (3D) culture environments, in part because of challenges associated with providing uniform and consistent 3D environments that can deliver a large range of physiological and pathophysiological strains to cells. Here, we report microscale magnetically actuated, cell-laden hydrogels (μMACs) for investigating the strain-induced cell response in 3D cultures. μMACs provide high-throughput arrays of defined 3D cellular microenvironments that undergo reversible, relatively homogeneous deformation following non-contact actuation under external magnetic fields. We present a technique that not only enables the application of these high strains (60%) to cells but also enables simplified microscopy of these specimens under tension. We apply the technique to reveal cellular strain-threshold and saturation behaviors that are substantially different from their 2D analogs, including spreading, proliferation, and differentiation. μMACs offer insights for mechanotransduction and may also provide a view of how cells respond to the extracellular matrix in a 3D manner.

show abstract

Section: Introductionmentioning

confidence: 99%

Magnetically actuated cell-laden microscale hydrogels for probing strain-induced cell responses in three dimensions

Huang

Gao

et al. 2016

NPG Asia Mater

View full text Add to dashboard Cite

show abstract

“…In a static setting the contractile stresses exerted by the cells lead to compaction of the unconstrained construct in all directions (Foolen et al 2012). If the construct is uniaxially constrained, the cytoskeleton of the cells orients in the constrained direction (Neiponice et al 2007) but surprisingly this preference remains even when cyclic strains are applied (Gauvin et al 2011;Nieponice et al 2007), i.e.…”

Section: Introductionmentioning

confidence: 99%

A thermodynamically motivated model for stress-fiber reorganization

Vigliotti

Ronan

Baaijens

et al. 2015

Biomech Model Mechanobiol

View full text Add to dashboard Cite

“…Moreover, images through the thickness of the top layer do not show a clear trend in the collagen orientation. Therefore, in this layer, the complex load-driven phenomena that have been shown to drive collagen orientation in 3D constructs (Foolen et al 2012) are not accounted for. For these reasons, the surface layers are approximately hypothesized to be isotropic.…”

Section: Discussionmentioning

confidence: 99%