The Influence of Androgen on the Male Accessory Reproductive Glands of the Guinea Pig: Studies on Growth, Histological Structure and Fructose and Citric Acid Secretion12

Ortiz, Evelina; Price, Dorothy; Williams-Ashman, H.G.; Banks, J.

doi:10.1210/endo-59-4-479

Cited by 27 publications

(22 citation statements)

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“…The relatively small response to testosterone of these young cultures is perhaps not unexpected since precocious sexual development cannot be induced by testosterone in some species. In this respect the mouse would appear to resemble the guinea pig rather than the rat (Ortiz et al, 1956;Gerall, 1958).…”

Section: Discussionmentioning

confidence: 99%

The Effects of Age on the Structure and Response to Oestrogens and Testosterone, of the Mouse Prostate in Organ Cultures

Lm¹

1959

Br J Cancer

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THE association between neoplasia and age is well seen in human prostatic cancer. In this disease, although the incidence increases with age, there is often a marked decline in biological malignancy (Franks, 1956), but it is uncertain whether the change is due to an alteration in the tumour or to endocrine or other changes in the host, which are known to accompany ageing. In addition the mechanism by which hormone-induced changes are produced in tumours or normal tissues in the intact animal remains obscure. By using the organ culture technique in which tissue can be maintained in an organised form in vitro for several weeks, it is possible to study the local tissue (or tumour) factors in isolation, and to observe the direct effects of specific hormones. There have been few reports however of studies of this type on adult tissues, except for those of Lasnitzki who, in a series of papers (Lasnitzki, 1951(Lasnitzki, , 1954(Lasnitzki, , 1955a, has described the effects of methylcholanthrene, oestrone, testosterone propionate and vitamin A on organ cultures of mouse prostate. The present paper records the effects in vitro of oestrogens and testosterone on the ventral prostate of mice of different ages, and is preliminary to a more detailed study of the human and rodent prostate. MATERIAL AND METHODSC57 mice were used in all except one series of experiments and killed by neck dislocation. The ventral prostate was removed and each lobe of the paired gland was divided into two, or occasionally, if the lobe was very large, into four pieces. Tissue from each mouse thus provided material for four or eight cultures, which were divided into four groups-one group grown on control medium and three on hormone-containing media. The cultures were explanted on rayon (4 pieces on 1 cm. squares) by Shaffer's (1956) modification of the standard watch glass technique (Fell and Robison, 1929). Each watch glass contained 2 pieces of rayon, i.e., 8 cultures, on 1.5 ml. of clotted medium, composed of 0.5 ml. each of chicken plasma, chick embryo extract and human serum. The hormones used were dissolved in the human serum, to which was also added sufficient chloromycetin to give a final concentration in the medium of approximately 003 mg./ml. Cultures were incubated at 37°C and the medium changed every 3 or 4 days. Eight cultures from each group were fixed after 1, 3, 7, 10 and 21 days in experiments 1, 2 and 3. The media used in each of these experiments were: 1. Control medium.2. Control medium + oestrone 2

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Section: Discussionmentioning

confidence: 99%

The Effects of Age on the Structure and Response to Oestrogens and Testosterone, of the Mouse Prostate in Organ Cultures

Lm¹

1959

Br J Cancer

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“…The male hormone in the blood maintains the normal ultrastructure and secretory function of the prostatic epithelial cells [Levey and Szego, 1955a;Ortiz et al;1956;Brandes, 1966Brandes, , 1974. Neural factors probably con trol the secretion of the glands.…”

Section: Structure and Functionmentioning

confidence: 99%

Fine Structural and Functional Study of the Prostatic Complex of the Guinea Pig

Wong¹,

Tse²

1981

Cells Tissues Organs

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The fine structure of the prostate gland of the guinea pig was studied by electron microscopy. The lateral lobe was characterized by tall columnar epithelium which formed various degrees of foldings. The epithelial cells had well developed granular endoplasmic reticulum (GER). The cisternae were closely packed and often arranged in concentric configuration in the basal cytoplasm. The apical cytoplasm was characterized by a large Golgi and prominent secretory granules. The luminal border typically contained microvilli. Fusion of the secretory granules with the luminal membrane were observed. The secretion in this lobe is believed to be triggered by a merocrine mechanism. The glandular cells of the dorsal prostate were characterized by well-developed and dilated GER. Most cells had no secretory granules and the luminal surface was endowed with microvilli. It is believed that in these cells the secretion is stored in the cisternae and may be discharged directly into the lumen. A small number of cells in the dorsal prostate showed large dense secretory granules in the apical region. Secretion in this group of cells, as in the cells of the lateral prostate, is believed to be produced by a merocrine mechanism. The coagulating gland on the other hand, had sparser GER and less dilated cisternae. The cells contained no secretory granules and usually had a small Golgi. Apical blebs were commonly found in these cells. Apical blebs have been observed to pinch off from the apical cytoplasm and to release the small sacs of secretory material into the lumen. This is an apocrine mechanism of secretion.

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“…The male accessory sex glands are well known for their dependency on androgen for normal structure and function [Levey and Szego, 1955a;Price et al, 1955;Brandes, 1966;, Castration is commonly used in order to study the effects of the male hormones on the accessory sex glands, because the removal of the androgenic stimulus by orchiectomy induces structural involution and an associated decrease or loss of functional activities [Ortiz et al, 1956;Szirmai and Van Der Linde, 1965;Brandes, 1966;Toner and Baillie, 1966;.…”

Section: Introductionmentioning

confidence: 99%

Structural study of the involution of the seminal vesicles of the guinea pig following orchiectomy

Tse¹,

Wong²

1980

Cells Tissues Organs

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The involution of the seminal vesicles of the guinea pig after castration has been studied by electron microscopy. The epithelial cells decreased in size and the basal lamina became more tortuous after castration. The most obvious changes occurred in the amount and configuration of the granular endoplasmic reticulum (GER). Besides a general decrease in GER, there was also a change from predominantly parallel arrays to a spiral or concentric arrangement, followed by dilation and vesiculation of the GER. There was an increase in glycogen and lipid contents of the epithelial cells. Large aggregates of glycogen particles were found associating with dilated GER and lipid droplets and these glycogen particles increased with the progression of involution. The Golgi complex became smaller and a slight increase in the number of autophagic and lysosomal granules was observed. Secretory granules decreased in size and quantity but persisted even at 4 months after the operation. The postcastration response of the seminal vesicles of the guinea pig was slow and incomplete and was different from that of the rat and mouse, where involution was virtually completed within 3 weeks. The slow and incomplete regression is not clear, but is believed to be related to the function of the adrenal glands which may provide the extratesticular source of hormone to partially maintain the gland after castration.

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The Influence of Androgen on the Male Accessory Reproductive Glands of the Guinea Pig: Studies on Growth, Histological Structure and Fructose and Citric Acid Secretion12

Cited by 27 publications

References 0 publications

The Effects of Age on the Structure and Response to Oestrogens and Testosterone, of the Mouse Prostate in Organ Cultures

The Effects of Age on the Structure and Response to Oestrogens and Testosterone, of the Mouse Prostate in Organ Cultures

Fine Structural and Functional Study of the Prostatic Complex of the Guinea Pig

Structural study of the involution of the seminal vesicles of the guinea pig following orchiectomy

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