The indices of nitrogen (II) oxide system in experimental hepatopulmonary syndrome

Krynytska, Іnna; Marushchak, Mariya

doi:10.15407/ubj90.05.091

Cited by 15 publications

(6 citation statements)

References 26 publications

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“…;Polyauk et al 2017). However, methods are not developed for examination of captopril from Caco-2 cell monolayers (Fujikawa et al 2005;Gertz et al 2010;Krynytska et al 2018). Caco-2 cells are a human colon epithelial cancer cell line used as a model of human intestinal absorption of drugs and other compounds.…”

Section: Introductionmentioning

confidence: 99%

Method development for the quantitative determination of captopril from Caco-2 cell monolayers by using LC-MS/MS

Logoyda¹,

Piponski²,

Коваленко³

et al. 2021

PHAR

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Aim. Caco-2 cells are a human colon epithelial cancer cell line used as a model of human intestinal absorption of drugs and other compounds. Although compounds were used in the original Caco-2 cells monolayer assays, compounds have been replaced in most laboratories by the use of liquid chromatography-mass spectrometry (LC-MS) and LC-tandem mass spectrometry (LC-MS/MS). Mass spectrometry not only eliminates the need for compounds, but permits the simultaneous measurement of multiple compounds. The measurement of multiple compounds per assay reduces the number of incubations that need to be carried out, thereby increasing the throughput of the experiments. Furthermore, LC-MS and LC-MS-MS add another dimension to Caco-2 assays by facilitating the investigation of the metabolism of compounds by Caco-2 cells. A simple, rapid LC-MS/MS method has been developed for determination of captopril from confluent Caco-2 monolayers and from aqueous solution. Materials and methods. Chromatography was achieved on Discovery C18, 50 × 2.1 mm, 5 μm column. Samples were chromatographed in a gradient mode (eluent A (acetonitrile – water – formic acid, 5 : 95 : 0.1 v/v), eluent B (acetonitrile – formic acid, 100 : 0.1 v/v)). The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.4 mL/min into the mass spectrometer ESI chamber. The sample volume was 5 μl. Results. Under these conditions, captopril was eluted at 1.42 min. A linear response function was established at 2 – 200 ng/mL. The regression equation for the analysis was y =0.0187x+0.000248 with coefficient of correction (r2) = 0.9993. According to the Caco-2 test results, captopril showed low permeability. It should be noted that the recovery value is 103.20%. The within-run coefficients of variation ranged between 0.321% and 0.541%. The within-run percentages of nominal concentrations ranged between 99.13% and 101.12%. The between-run coefficients of variation ranged between 0.314% and 0.663%. The between-run percentages of nominal concentrations ranged between 99.17% and 101.03%.The assay values on both the occasions (intra- and inter-day) were found to be within the accepted limits. Conclusion. From results of analysis, it can be concluded that developed method is simple and rapid for determination of captopril from confluent Caco-2 monolayers and from aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for examination of captopril from Caco-2 cell monolayers.

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Section: Introductionmentioning

confidence: 99%

Method development for the quantitative determination of captopril from Caco-2 cell monolayers by using LC-MS/MS

Logoyda¹,

Piponski²,

Коваленко³

et al. 2021

PHAR

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“…In a similar study, Degano et al found that concentration of NO in exhaled air of patients with cirrhosis was three times higher than that in healthy persons 14 . Krynytska and Marushchak found a significant increase in total NOS activity and concentration of NO 2-+ NO 3in lung tissue of rats with HPS 15 . Using flow cytometry, which allowed to differentiate the alveolar and bronchial origin of NO, it was determined that the increased formation of NO mainly took place in the alveoli 16 .…”

Section: Nitric Oxide-mediated Vasodilationmentioning

confidence: 93%

Hepatopulmonary syndrome: proposed mediators of pulmonary vasodilation

Krynytska¹,

Marushchak²,

Odnorih³

et al. 2018

Arch Balk Med Union

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“…The hepatopulmonary syndrome (HPS) is an important vascular complication because of systemic hypoxemia in patients with cirrhosis and/or portal hypertension. It is formed by a clinical triad of arterial oxygenation abnormalities induced by intrapulmonary vascular dilatations with liver disease [1][2][3] . True HPS is estimated to occur in at least 10% of all patients with cirrhosis and portal hypertension 4 .…”

Section: Introductionmentioning

confidence: 99%

The indices of endogenous intoxication in rats with different models of hepatopulmonary syndrome

Krynytska¹,

Odnorih²,

Savchenko³

2019

Arch Balk Med Union

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Indices de l'intoxication endogène chez des rats aux différents types de syndrome hépato-pulmonaire L'objectif de l'étude. La pathogenèse du syndrome hépato-pulmonaire (SHP) n'a pas été clarifiée, mais comme le développement du SHP est basé sur la dilatation interne des capillaires pulmonaires, les recherches suggèrent que le SHP est causé par l'action prolongée de composés biologiquement actifs sur les vaisseaux sanguins du circuit pulmonaire et, un syndrome de réponse inflammatoire systémique a également été impliqué dans la pathogenèse du SHP. L'objectif de cette étude était d'évaluer les indices d'intoxication endogène et d'inflammation dans le sérum sanguin et le tissu pulmonaire d'animaux présentant différents types de syndrome hépato-pulmonaire. Méthodes. Le premier modèle expérimental de SHP a été réalisé en imposant une double ligature sur le canal biliaire principal et en le disséquant à l'aide d'un scalpel. Les animaux du deuxième groupe

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The indices of nitrogen (II) oxide system in experimental hepatopulmonary syndrome

Cited by 15 publications

References 26 publications

Method development for the quantitative determination of captopril from Caco-2 cell monolayers by using LC-MS/MS

Method development for the quantitative determination of captopril from Caco-2 cell monolayers by using LC-MS/MS

Hepatopulmonary syndrome: proposed mediators of pulmonary vasodilation

The indices of endogenous intoxication in rats with different models of hepatopulmonary syndrome

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