The in vitro transport of calcium by the frog intestine and the effect of vitamin D3

DIURNAL VARIATIONS IN THE INFLUENCE OF THE ULTIMOBRANCHIAL GLANDS ON CALCIUM HOMEOSTASIS IN THE FROG ( RANA PIPIENS )

“…One ligature was placed at the pyloric end and a second ligature one third of the way along the gut to give a duodenal segment which in all assays was incubated for 30 min at 21-5 ±0-5°C in 100 ml buffer (Robertson, 19756). The data obtained on the linear portion of the uptake curve reflected the 'concentrative capacity', in contrast to a 'final concentration' and was expressed as the ratio between the concentrations of calcium in the serosal and mucosal compartments of the gut (S : M ratio), which had an initial value of 1.…”

DIURNAL VARIATIONS IN THE INFLUENCE OF THE ULTIMOBRANCHIAL GLANDS ON CALCIUM HOMEOSTASIS IN THE FROG ( RANA PIPIENS )

“…One ligature was placed at the pyloric end and a second ligature one third of the way along the gut to give a duodenal segment which in all assays was incubated for 30 min at 21-5 ±0-5°C in 100 ml buffer (Robertson, 19756). The data obtained on the linear portion of the uptake curve reflected the 'concentrative capacity', in contrast to a 'final concentration' and was expressed as the ratio between the concentrations of calcium in the serosal and mucosal compartments of the gut (S : M ratio), which had an initial value of 1.…”

DIURNAL VARIATIONS IN THE INFLUENCE OF THE ULTIMOBRANCHIAL GLANDS ON CALCIUM HOMEOSTASIS IN THE FROG ( RANA PIPIENS )

“…One ligature was placed at the pyloric end and a second ligature one third of the way along the gut to give a duodenal segment which in all assays was incubated for 30 min at 21-5 ±0-5°C in 100 ml buffer (Robertson, 19756). The data obtained on the linear portion of the uptake curve reflected the 'concentrative capacity', in contrast to a 'final concentration' and was expressed as the ratio between the concentrations of calcium in the serosal and mucosal compartments of the gut (S : M ratio), which had an initial value of 1.…”

DIURNAL VARIATIONS IN THE INFLUENCE OF THE ULTIMOBRANCHIAL GLANDS ON CALCIUM HOMEOSTASIS IN THE FROG ( RANA PIPIENS )

“…One ligature was placed at the pyloric end and a second ligature one third of the way along the gut to give a duodenal segment which in all assays was incubated for 30 min at 21-5 ±0-5°C in 100 ml buffer (Robertson, 19756). The data obtained on the linear portion of the uptake curve reflected the 'concentrative capacity', in contrast to a 'final concentration' and was expressed as the ratio between the concentrations of calcium in the serosal and mucosal compartments of the gut (S : M ratio), which had an initial value of 1.…”