The in vitro effect of dandelions antioxidants on microsomal lipid peroxidation

Abstract

Dandelions have long been used in herbal medicine for their choleretic, diuretic, antiinflammatory, appetite‐stimulating and laxative properties. An antioxidant property can be supposed as a basis of their­therapeutic effects. To understand the mechanism of the drug's action, the effects of natural extracts on a microsomal fraction of rat liver were examined. The extracts diminished the enzymatically induced­lipid peroxidation and reduced the cytochrome c with and without NADPH in a concentration dependent manner. Copyright © 2000 John Wiley & Sons, Ltd.

The modulatory action of taraxacum officinale on the glutathione system

“…Also, roots and leaves extracts can stimulate the NADPH-cytochrome P-450 reductase activity, the hydrogen-donating ability, reducing power property and radical scavenging capacity of TO. The higher hydrogen donor, reducing agent and hydrogen peroxide scavenger capability of the leaf extract correlates with the approximately 3 times higher polyphenol content compared to radix extract [15].…”

“…Taraxacum officinale is a vigorous plant found worldwide, considered a weed by most gardeners and has been used for many purposes in traditional medicine. This plant is well known demonstrating antioxidant, anti-inflammatory and antitumor properties [15]. The content and effectiveness of TOL and TOR depends of many factors: the period of harvesting, altitudes, soil type, environmental factors (temperature, photoperiod and rainfall) and parts of plant used for extracting.…”

The modulatory action of taraxacum officinale on the glutathione system

“…Also, roots and leaves extracts can stimulate the NADPH-cytochrome P-450 reductase activity, the hydrogen-donating ability, reducing power property and radical scavenging capacity of TO. The higher hydrogen donor, reducing agent and hydrogen peroxide scavenger capability of the leaf extract correlates with the approximately 3 times higher polyphenol content compared to radix extract [15].…”

“…Taraxacum officinale is a vigorous plant found worldwide, considered a weed by most gardeners and has been used for many purposes in traditional medicine. This plant is well known demonstrating antioxidant, anti-inflammatory and antitumor properties [15]. The content and effectiveness of TOL and TOR depends of many factors: the period of harvesting, altitudes, soil type, environmental factors (temperature, photoperiod and rainfall) and parts of plant used for extracting.…”

Standardization of roots of Taraxacum officinale

“…Preliminary phytochemical screening of root of Taraxacum officinale gave the colors with modifications according to colors of extract indicating the presence of the various active phytoconstituents metabolites as follows: carbohydrate (Molish's violet ring at the junction of two liquids, Fehling's yellow to red, Bendict's greenish yellow to red), protein (Biuret violet, Xanthoprotein yellow precipitates), amino acids (Ninhydrin purple colour) glycosides (Legal pinkish red), steroids and sterol (Liebermann-Burchard purple / violet , Salkowski red color ring at junction of two layer) tannins (lead acetate white precipitates, Ferric chloride bluish black) and saponin (Foam test persistent of white foam at upper layer). Root extract of Taraxacum officinale revealed the presence of cardiac glycosides, terpenoids (antiviral activity), phenols (Immuno modulatory and antihyperglycemic) (Kenny et al, 2015) , tannins (wound healing activity) (Ajaz et al, 2019), flavonoids (antioxidant and antiinflammatory activity) (Hagymási et al, 2000), steroids and sterols (Antihyperglycemic and Anti inflammatory activity) (Petlevski et al, 2003) (Jones and Persaud, 1998) carbohydrates, proteins and amino acids with absence of alkaloids and fixed oil. Also similar finding reported in the other species of the same family (Jaramillo-Jaramillo et al, 2016).…”

Can Dandelion’s Phenolic Compounds Suppress Glioblastoma Cells Viability?

“…(2000) reported a 3-fold higher content of polyphenols in the water extract from the leaves (9.9g%) than from the roots, which may underline the higher efficiency of TO leaves as a hydrogen donor (I 50 = 160 μg), reducing agent (740 ASE mg -1 ) and H 2 O 2 scavenger (I 50 =155 μg) compared to root extracts 22 . Our results confirm the higher content of polyphenols in the leaves, which was about 4.6 (p=0.007) times higher than in the roots.…”

The modulatory action of taraxacum officinale on the glutathione system

“…Also, roots and leaves extracts can stimulate the NADPH-cytochrome P-450 reductase activity, the hydrogen-donating ability, reducing power property and radical scavenging capacity of TO. The higher hydrogen donor, reducing agent and hydrogen peroxide scavenger capability of the leaf extract correlates with the approximately 3 times higher polyphenol content compared to radix extract [15].…”

“…Taraxacum officinale is a vigorous plant found worldwide, considered a weed by most gardeners and has been used for many purposes in traditional medicine. This plant is well known demonstrating antioxidant, anti-inflammatory and antitumor properties [15]. The content and effectiveness of TOL and TOR depends of many factors: the period of harvesting, altitudes, soil type, environmental factors (temperature, photoperiod and rainfall) and parts of plant used for extracting.…”

Standardization of roots of Taraxacum officinale

“…Preliminary phytochemical screening of root of Taraxacum officinale gave the colors with modifications according to colors of extract indicating the presence of the various active phytoconstituents metabolites as follows: carbohydrate (Molish's violet ring at the junction of two liquids, Fehling's yellow to red, Bendict's greenish yellow to red), protein (Biuret violet, Xanthoprotein yellow precipitates), amino acids (Ninhydrin purple colour) glycosides (Legal pinkish red), steroids and sterol (Liebermann-Burchard purple / violet , Salkowski red color ring at junction of two layer) tannins (lead acetate white precipitates, Ferric chloride bluish black) and saponin (Foam test persistent of white foam at upper layer). Root extract of Taraxacum officinale revealed the presence of cardiac glycosides, terpenoids (antiviral activity), phenols (Immuno modulatory and antihyperglycemic) (Kenny et al, 2015) , tannins (wound healing activity) (Ajaz et al, 2019), flavonoids (antioxidant and antiinflammatory activity) (Hagymási et al, 2000), steroids and sterols (Antihyperglycemic and Anti inflammatory activity) (Petlevski et al, 2003) (Jones and Persaud, 1998) carbohydrates, proteins and amino acids with absence of alkaloids and fixed oil. Also similar finding reported in the other species of the same family (Jaramillo-Jaramillo et al, 2016).…”

Can Dandelion’s Phenolic Compounds Suppress Glioblastoma Cells Viability?

“…(2000) reported a 3-fold higher content of polyphenols in the water extract from the leaves (9.9g%) than from the roots, which may underline the higher efficiency of TO leaves as a hydrogen donor (I 50 = 160 μg), reducing agent (740 ASE mg -1 ) and H 2 O 2 scavenger (I 50 =155 μg) compared to root extracts 22 . Our results confirm the higher content of polyphenols in the leaves, which was about 4.6 (p=0.007) times higher than in the roots.…”

The modulatory action of taraxacum officinale on the glutathione system

“…Also, roots and leaves extracts can stimulate the NADPH-cytochrome P-450 reductase activity, the hydrogen-donating ability, reducing power property and radical scavenging capacity of TO. The higher hydrogen donor, reducing agent and hydrogen peroxide scavenger capability of the leaf extract correlates with the approximately 3 times higher polyphenol content compared to radix extract [15].…”

“…Taraxacum officinale is a vigorous plant found worldwide, considered a weed by most gardeners and has been used for many purposes in traditional medicine. This plant is well known demonstrating antioxidant, anti-inflammatory and antitumor properties [15]. The content and effectiveness of TOL and TOR depends of many factors: the period of harvesting, altitudes, soil type, environmental factors (temperature, photoperiod and rainfall) and parts of plant used for extracting.…”

Standardization of roots of Taraxacum officinale

“…Preliminary phytochemical screening of root of Taraxacum officinale gave the colors with modifications according to colors of extract indicating the presence of the various active phytoconstituents metabolites as follows: carbohydrate (Molish's violet ring at the junction of two liquids, Fehling's yellow to red, Bendict's greenish yellow to red), protein (Biuret violet, Xanthoprotein yellow precipitates), amino acids (Ninhydrin purple colour) glycosides (Legal pinkish red), steroids and sterol (Liebermann-Burchard purple / violet , Salkowski red color ring at junction of two layer) tannins (lead acetate white precipitates, Ferric chloride bluish black) and saponin (Foam test persistent of white foam at upper layer). Root extract of Taraxacum officinale revealed the presence of cardiac glycosides, terpenoids (antiviral activity), phenols (Immuno modulatory and antihyperglycemic) (Kenny et al, 2015) , tannins (wound healing activity) (Ajaz et al, 2019), flavonoids (antioxidant and antiinflammatory activity) (Hagymási et al, 2000), steroids and sterols (Antihyperglycemic and Anti inflammatory activity) (Petlevski et al, 2003) (Jones and Persaud, 1998) carbohydrates, proteins and amino acids with absence of alkaloids and fixed oil. Also similar finding reported in the other species of the same family (Jaramillo-Jaramillo et al, 2016).…”

Can Dandelion’s Phenolic Compounds Suppress Glioblastoma Cells Viability?

“…(2000) reported a 3-fold higher content of polyphenols in the water extract from the leaves (9.9g%) than from the roots, which may underline the higher efficiency of TO leaves as a hydrogen donor (I 50 = 160 μg), reducing agent (740 ASE mg -1 ) and H 2 O 2 scavenger (I 50 =155 μg) compared to root extracts 22 . Our results confirm the higher content of polyphenols in the leaves, which was about 4.6 (p=0.007) times higher than in the roots.…”