The in vitro cultivation of Treponema pallidum. Corroborative studies.

Foster, J W; Kellogg, Douglas S.; Clark, John W.; Balows, Albert

doi:10.1136/sti.53.6.338

Cited by 4 publications

(4 citation statements)

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“…Survival of T. pertenue in gradient tissue cultures. Other investigators have shown that nonvirulent, cultivable treponemes did not attach to tissue cultures (2,3,6), and it had been assumed that the phenomenon of attachment was characteristic of pathogenic, noncultivable 100 (14) 90 (9) 54 (4) 22 (2) 0 (0) 23 100 (4) 33 (2) 0 (1) 0 (2) 0 (3) 29 85 (2) a SflEp, Cottontail rabbit epithelium cells; HAF, human adult foreskin fibroblast cells; RT, rabbit testis fibroblast cells; NME, nude (nu/nu) mouse ear fibroblast cells; FRS, fetal rabbit skin fibroblast cells. Number of experiments: SflEp, 5; HAF, 2; RT, 2; NME, 2; and FRS, 2.…”

Section: Resultsmentioning

confidence: 99%

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Comparative Behavior of Virulent Strains of Treponema pallidum and Treponema pertenue in Gradient Cultures of Various Mammalian Cells

1979

Infect Immun

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Two strains of virulent Treponema pallidum and two of virulent T. pertenue were investigated for their ability to attach to and survive in gradient cultures of five different mammalian cells under aerobic conditions. The strains of T. pallidum studied were the high-rabbit-passage Nichols and the low-rabbit-passage KKJ. The former was known to readily attach to cottontail rabbit epithelial cells (SflEp) and to survive in the virulent state for up to 21 days. We therefore compared attachment of the other virulent treponemes with that of T. pallidum (Nichols). The KKJ strain of T. pallidum behaved in a fashion similar to T. pallidum (Nichols) in all of the cultures. Both strains exhibited preferential attachment to cells of Sf1Ep and those derived from the ear of a nude athymic (nu/nu) mouse. In these cultures, we observed a consistent threeto fivefold increase in attached treponemes up to 12 days after initial inoculation. The strains of T. pertenue were the human-derived Gauthier and cynocephalus-derived FB. These two strains of T. pertenue also attached to cells of all five types of cultures, but in smaller numbers than were seen with T. pallidum and equally to all of the cultures. Neither preferential attachment to SflEp and nude mouse ear cells nor increased attachment with time was seen.

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Section: Resultsmentioning

confidence: 99%

“…Recently, Jones et al (8) reported the successful cultivation of virulent T. pallidum through nine passages in tissue cultures of baby hamster kidney. Foster et al (4), despite intensive efforts, failed to confirm the results of Jones and his colleagues.…”

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confidence: 94%

Comparative Behavior of Virulent Strains of Treponema pallidum and Treponema pertenue in Gradient Cultures of Various Mammalian Cells

1979

Infect Immun

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“…In 1976, Jones et al 15claimed to have demonstrated replication and subculture of pathogenic T. pallidum in cultures of baby hamster kidney, but Foster et al (11) failed to corroborate these results. Sandok and his colleagues (21) demonstrated what they called "unsustained multiplication" of T. pallidum in cultures under reduced oxygen tension, both in the presence and absence of mammalian cells.…”

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Cultivation of Virulent Treponema pallidum in Tissue Culture

1981

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In a series of seven experiments, the virulent Nichols strain of Treponema pallidum was shown to attach and replicate on the surface of tissue culture cells of cottontail rabbit epithelium (SflEp) growing in conventional monolayer cultures under an atmosphere of 1.5% oxygen. Five days after inoculation of 106 T. pallidum, the number of treponemes had increased to between 8 x 106 and 2.59 x 107. The viability of harvested organisms ranged from 86 to 97%. The number of T. pallidum continued to increase, generally reaching a plateau between days 9 and 12 of incubation, with increases ranging up to 100-fold and averaging 49fold. There appeared to be a ceiling of multiplication of about 2 x 108 irrespective of the inoculum, which ranged from 106 to 108 T. pallidum. Concurrent deoxyribonucleic acid assays were performed on the cultures containing T. pallidum to obtain further evidence of replication. Significant increases in treponemal deoxyribonucleic acid were observed when the inocula ranged from 106 to 107, with the greatest increases, as might be expected, being in the former group. There was also excellent correlation in the amount of deoxyribonucleic acid per treponeme; 908

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“…The paper by Jones et al (1976) despite its optimistic title, was greeted with muted cheers, for many questions remained unanswered. In this issue (pages 338-339), comes the dismaying report by Foster et al (1977) of the Center for Disease Control, Atlanta, USA, who have been unable to repeat the results of Jones and his colleagues.…”

Section: Editorialmentioning

confidence: 99%

Cultivation of Treponema pallidum.

Turner¹

1977

Sexually Transmitted Infections

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The in vitro cultivation of Treponema pallidum. Corroborative studies.

Cited by 4 publications

References 4 publications

Comparative Behavior of Virulent Strains of Treponema pallidum and Treponema pertenue in Gradient Cultures of Various Mammalian Cells

Comparative Behavior of Virulent Strains of Treponema pallidum and Treponema pertenue in Gradient Cultures of Various Mammalian Cells

Cultivation of Virulent Treponema pallidum in Tissue Culture

Cultivation of Treponema pallidum.

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