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“…In accordance with earlier work on man [5,13], on rabbits [25] and on guinea pigs [17], it was found that normal human serum and plasma was chemotactic for neutro- phils. However, in contrast to effects on monocytes, already undiluted serum dis played considerable activity.…”
Section: Neutrophil Chemotactic Activity Of Normal Human Serum and Plsupporting
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“…In accordance with earlier work on man [5,13], on rabbits [25] and on guinea pigs [17], it was found that normal human serum and plasma was chemotactic for neutro- phils. However, in contrast to effects on monocytes, already undiluted serum dis played considerable activity.…”
Section: Neutrophil Chemotactic Activity Of Normal Human Serum and Plsupporting
“…Mononuclear as well as neutrophil activity was found in rab bit serum and/or plasma [2,25], in guinea pig serum and plasma [7,17] and neutro phil activity in human serum and plasma [5,13]. The present work confirms that human serum and plasma are chemotactic for hu man neutrophils.…”
Section: Discussionsupporting
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“…Opsonized organisms were removed from test sera by centrifugation at 10,000 X g (4°C) for 15 min. The supernatant of each opsonic mixture was transferred to sterile vials and heated for 30 min at 56°C to avoid nonspecific complement activation (23). Aliquots were then diluted to a final concentration of 12%% (v/v) in DPBS for use in chemotaxis assays.…”
Section: Isolation Of Pmnmentioning