The improvement of bioactive secondary metabolites accumulation in Rumex gmelini Turcz through co-culture with endophytic fungi

Abstract

Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer. Among these fungi, Aspergillus sp. showed the most significant enhancement on bioactive components accumulation in RGT seedlings. When inoculated Aspergillus sp. spores into media of RGT seedlings that had taken root for 20 d, and made spore concentration in co-culture medium was 1 × 104 mL–1, after co-cultured for 12 d, the yield of chrysophaein, resveratrol, chrysophanol, emodin and physcion were 3.52-, 3.70-, 3.60-, 4.25-, 3.85-fold of the control group. The extreme value of musizin yield was 0.289 mg, which was not detected in the control groups. The results indicated that co-culture with endophytic fungi could significantly enhance bioactive secondary metabolites production of RGT seedlings.

Cannabis Microbiome and the Role of Endophytes in Modulating the Production of Secondary Metabolites: An Overview

“…Accumulated evidence established that endophytes are capable of eliciting physiological plant responses, which in turn influence the production of secondary metabolites in the host plant [110]. The production of bioactive secondary metabolites of Rumex gmelini seedlings is enhanced through coculture with endophytic fungi [111]. An endophytic bacterium Pseudonocardia sp.…”

Molecular Identification of Endophytic Fungi and Their Pathogenicity Evaluation Against Dendrobium nobile and Dendrobium officinale

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KARAKTERISASI BAKTERI ENDOFIT TANAMAN PURWOCENG SEBAGAI PENGHASIL SENYAWA STEROID DAN ANTIPATOGEN / Endophytic Bacteria From Purwoceng as Steroid and Antipatogenic Compounds Producers

“…Isolat bakteri endofit yang telah diperoleh dari hasil isolasi tanaman purwoceng diinokulasikan ke media NB sebanyak 20 ml, kemudian dishaker dengan kecepatan 125 rpm selama 3 hari pada suhu kamar. Disamping itu dilakukan pembuatan media perakaran yaitu media MS (1/4 MS + NAA 0,5 ppm) dan di atas media dibuat jembatan dari kertas filter (Ding et al 2017) yang dilakukan seperti pada gambar di bawah ini, sebagai tempat pertumbuhan kultur tanaman purwoceng.…”