A comprehensive laboratory-style teaching activity involving
the
synthesis, characterization, and analysis of structure–activity
relationships of metal–organic framework (MOF) nanozymes was
developed specifically for undergraduates at Tianjin University. The
students acquired familiarity with the general approach in synthesizing
MOFs by solvothermal method using substituted MIL-101(Fe)-X (MIL is
an acronym for Materials of Institute Lavoisier). Several modern characterization
methods were taught, including powder X-ray diffraction and fluorescence
spectroscopy for assessing MIL-101(Fe)-X crystal structures and superoxide
dismutase (SOD)-like activities. In particular, students were guided
by the instructor to investigate the relationship between structural
differences in MOFs caused by ligand changes and their SOD-like activities.
The Hammett relationships were later demonstrated to be an accurate
method of quantifying structure–activity relationships of MIL-101(Fe)-X
nanozymes. By participating in this laboratory experience, students
are able to improve their scientific thinking, practical abilities,
and teamwork. Additionally, a fundamental principle of material design
can be conveyed to undergraduates based on the structure–activity
relationship, thereby deepening their understanding of nanozymes and
the course’s content.