The Importance of Extracellular Matrix in Skeletal Muscle Development and Function

Grzelkowska-Kowalczyk, Katarzyna

doi:10.5772/62230

Cited by 32 publications

(29 citation statements)

References 66 publications

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“…The following ischemic conditions favor fibroblast proliferation, fibrosis, and fibrotic scar tissue formation, which leads to further degeneration of the muscle [ 25 ]. The ECM composition and extent in scar tissues affect many aspects of myogenesis, muscle function, and reinnervation [ 26 ]. It can severely constrain motion and thereby aggravate the consequences of muscle tissue loss.…”

Section: Introductionmentioning

confidence: 99%

Current Methods for Skeletal Muscle Tissue Repair and Regeneration

Liu

Saul

Böker

et al. 2018

BioMed Research International

114

139

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Skeletal muscle has the capacity of regeneration after injury. However, for large volumes of muscle loss, this regeneration needs interventional support. Consequently, muscle injury provides an ongoing reconstructive and regenerative challenge in clinical work. To promote muscle repair and regeneration, different strategies have been developed within the last century and especially during the last few decades, including surgical techniques, physical therapy, biomaterials, and muscular tissue engineering as well as cell therapy. Still, there is a great need to develop new methods and materials, which promote skeletal muscle repair and functional regeneration. In this review, we give a comprehensive overview over the epidemiology of muscle tissue loss, highlight current strategies in clinical treatment, and discuss novel methods for muscle regeneration and challenges for their future clinical translation.

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Section: Introductionmentioning

confidence: 99%

Current Methods for Skeletal Muscle Tissue Repair and Regeneration

Liu

Saul

Böker

et al. 2018

BioMed Research International

114

139

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“…It is well known that muscle homeostasis relies on the relation between the different cell types present in the muscle tissue and their microenvironment. Thus, communication between the ECM and muscle cells is essential for gene expression, cell proliferation, adhesion, and differentiation [1].…”

Section: Introductionmentioning

confidence: 99%

A Novel Functional In Vitro Model that Recapitulates Human Muscle Disorders

Toral-Ojeda¹,

Aldanondo²,

Lasa-Elgarresta³

et al. 2018

Muscle Cell and Tissue - Current Status of Research Field

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Here, we aim to address the increasing need for a suitable human muscle in vitro model in order to advance in the knowledge of muscle pathophysiology and test novel therapies for muscle disorders. Our model is based on a simple 2D culture method that yields highly mature human myotubes under optimized environmental conditions. Culture conditions that produced functional and contractile human myotubes with an extended lifetime consisted in extracellular matrix overlay and addition of several trophic factors to the differentiation medium. In this work, we describe the generation of suitable models of muscular dystrophies (limb-girdle muscular dystrophy type 2A-LGMD2A and Duchenne) by silencing expression of key proteins in these myotubes. Western blot and immunocytochemical analyses demonstrated similar features between our knockdown human myotubes and dystrophic muscles in vivo, which support the general validity of our cellular models. We also found that both dystrophic models present higher resting cytosolic Ca 2+ levels than controls, which support a common underlying deficit in calcium homeostasis. This novel human in vitro system would allow for high-throughput screening of new treatments for these muscular dystrophies as well as for other neuromuscular disorders. In addition, our model could be used to advance in our understanding of human skeletal muscle pathophysiology.

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“…The ECM, i.e., the acellular part of the tissue that surrounds the cells, is a key component of all tissues. The skeletal muscle ECM is responsible for tissue elasticity, generates adhesion points for cell adherence, provides cells with a 3D environment and regulates cell proliferation, differentiation, migration, morphology and alignment via biochemical and biophysical cues (Tse and Engler, 2011;Hausman, 2012;Chaturvedi et al, 2015;Fuoco et al, 2016;Grzelkowska-Kowalczyk, 2016). The ECM composition and roles are not constant, but change during the muscle development in a process called fibrogenesis, in parallel to myogenesis and adipogenesis (Thorsteinsdóttir et al, 2011;Yan et al, 2013;Miao et al, 2016).…”

Section: Fibroblasts and Ecmmentioning

confidence: 99%

Tissue Engineering for Clean Meat Production

Ben-Arye

Levenberg

2019

Front. Sustain. Food Syst.

188

134

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Increasing public awareness of foodborne illnesses, factory farming, and the ecological footprint of the meat industry, has generated the need for animal-free meat alternatives. In the last decade, scientists have begun to leverage the knowledge and tools accumulated in the fields of stem cells and tissue engineering toward the development of cell-based meat (i.e., clean meat). In tissue engineering, the physical and biochemical features of the native tissue can be mimicked; cells and biomaterials are integrated under suitable culture conditions to form mature tissues. More specifically, in skeletal muscle tissue engineering, a plurality of cell types can be co-cultured on a 3D scaffold to generate muscle fibers, blood vessels and a dense extracellular matrix (ECM). This review focuses on tissue engineering of skeletal muscle and the adjustments needed for clean meat development. We discuss the skeletal muscle structure and composition, and elaborate on cell types from farm animals that have the potential to recapitulate the muscle ECM, blood vessels, muscle fibers and fat deposits. We also review relevant biomaterials, primarily for fabricating scaffolds that can mimic the intramuscular connective tissues, as well as gene expression studies on the biological pathways that influence meat quality.

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The Importance of Extracellular Matrix in Skeletal Muscle Development and Function

Cited by 32 publications

References 66 publications

Current Methods for Skeletal Muscle Tissue Repair and Regeneration

Current Methods for Skeletal Muscle Tissue Repair and Regeneration

A Novel Functional In Vitro Model that Recapitulates Human Muscle Disorders

Tissue Engineering for Clean Meat Production

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